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Biochemicals & Reagents on Zageno

HP Ampicillin™ G-Biosciences
Reagent Type Antibiotics Applicable Processes Gene modification, Gene transfer, Molecular cloning, Selection Quantity 10 Tubes, Vials
From $ 153.00 (10 Vials)
Sizes 1 (10 Vials)
Catalog IDs GD-6060
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TypeOne™ Restriction Inhibitor Epicentre
Reagent Type Inhibitors Applicable Processes Gene modification, Gene transfer, Inhibition, Molecular cloning Quantity 100 μg
From $ 178.00 (100 µg)
Sizes 1 (100 µg)
Catalog IDs TY0261H
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RNaseOFF Ribonuclease Inhibitor Applied Biological Materials
Reagent Type Inhibitors Applicable Processes cDNA synthesis, In vitro transcription, Inhibition, Real-time PCR Quantity 4000 U
From $ 80.00 (100 µl)
Sizes 1 (100 µl)
Catalog IDs G138
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TurboFectin 8.0 Origene
Reagent Type Solutions Applicable Processes Gene transfer Quantity 1 ml
From $ 420.00 (1 Vial)
Sizes 2 (1 - 5 Vial)
Catalog IDs TF81001, TF81005
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Fetal Bovine Serum, certified, heat inactivated, US origin Gibco
Reagent Type Solutions Applicable Processes Cultivation Quantity 100 ml, 500 ml
From $ 192.00 (100 ml)
Sizes 2 (100 - 500 ml)
Catalog IDs 10082139, 10082147
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D-Valine Santa Cruz Biotechnology
Reagent Type Amino acids Applicable Processes Synthesis Quantity 5 g
From $ 31.00 (5 g)
Sizes 1 (5 g)
Catalog IDs sc-257298
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L-Valine Santa Cruz Biotechnology
Reagent Type Amino acids Applicable Processes Synthesis Quantity 25 g
From $ 25.00 (25 g)
Sizes 1 (25 g)
Catalog IDs sc-397358
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X-Gal Substrate OZ Biosciences
Reagent Type Substrates Applicable Processes Enzymatic reaction Quantity 1 g
From $ 101.00 (1 g)
Sizes 1 (1 g)
Catalog IDs XG31000
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Formalin, HARLECO™, EMD Millipore Fisher Scientific
Reagent Type Other reagents Applicable Processes Sample preservation Quantity 4 L
From $ 62.50 (4 L)
Sizes 1 (4 L)
Catalog IDs M6534685
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Chloroform (Approx. 0.75% Ethanol as Preservative/Molecular Biology) Fisher Scientific
Reagent Type Solutions Applicable Processes Purification Quantity 1000 ml, 6 x 1000 ml
From $ 157.56 (1000 ml)
Sizes 2 (1000 - 6000 ml)
Catalog IDs BP1145-1, BP1145-1
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Actin Antibody (C-2) Santa Cruz Biotechnology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow cytometry, ELISA Quantity /
From $ 279.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs sc-8432
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Protease inhibitors

During the process of protein extraction, cells are lysed, and endogenous proteases are released which break down and degrade the cell proteins. As a result, the yield of proteins at the end of the extraction process will be negatively affected. Therefore it is important to introduce protease inhibitors within the cell lysates to preserve the protein content. There are multiple groups of protease; the most characterized protease groups include serine protease, cysteine protease, aspartic protease, and metalloprotease. For this reason, protease inhibitor cocktails that include a broad spectrum of inhibitors are needed to target multiple protease groups to ensure complete inhibition of protease activity.

RNase inhibitors

During RNA isolation and purification RNase can be co-purified with RNA, and this can threaten the fate of RNA, lowering the overall yield. The RNA sample can also be contaminated with RNase by lab apparatus such as test tubes, pipette tips, and other reagents. The RNase family contain several groups of RNase, and therefore it is important that the RNase inhibitors that are used in RNA isolation can inhibit the activity of multiple types of RNase. The RNase inhibitors form complexes with RNases to prevent them interacting with their substrate RNA.

Lysogeny broth

Lysogeny broth, which is also known as Luria broth, Lennox broth, or Luria-Bertani medium, is growth medium rich in nutrients that support the growth of bacteria, especially Escherichia coli. The applications of lysogeny broth in life science research are broad due to its requirement for the production of plasmids and recombinant proteins. Although the compositions of lysogeny broth are always slightly different between labs and companies, generally they share some common vitamins, peptides, trace elements, and minerals. As lysogeny broth is prepared in liquid form, the addition of agar powder is necessary for the formation of gel required for growing bacteria.

Staining reagents

Staining reagents are another commonly used biochemical reagent for characterizing and identifying specific features of cells. Some commonly used staining reagents include crystal violet, hematoxylin, and eosin. Crystal violet is used for gram staining which can help classify bacteria into their two main groups (Gram-positive and Gram-negative). Gram-positive bacteria contain peptidoglycan in their cell wall, which can hold the crystal violet dye and therefore become stained. Hematoxylin and Eosin (H&E), which can stain cell nuclei and cytoplasm respectively are used in H&E staining; a gold standard in histology (or histopathology) for examining tissue samples from patients or sample organisms.

Antibiotics

Antibiotics refer to any agents that can kill bacteria or inhibit their growth. Apart from medical uses, the application of antibiotics is broad. For example, antibiotics can be used to select for cells that have picked up a recombinant plasmid DNA containing an antibiotic resistance gene. Although antibiotics are known to originate from natural products, modern antibiotics are mostly synthetic.

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