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Enzymes on Zageno

Proteinase K Bioline
Enzyme Type Protease Templates Proteins Applicable Processes Purification
From (on request)
No sizes available at the moment.
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AatII Vivantis
Enzyme Type Restriction enzyme Templates dsDNA Applicable Processes Enzymatic reaction
From (on request)
Sizes 1 (200 Units)
Catalog IDs RE1100
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AccB1 I {HgiCI} Vivantis
Enzyme Type Restriction enzyme Templates dsDNA Applicable Processes Enzymatic reaction
From (on request)
Sizes 1 (500 Units)
Catalog IDs RE1106
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AfiI {BsiYI} Vivantis
Enzyme Type Restriction enzyme Templates dsDNA Applicable Processes Enzymatic reaction
From (on request)
Sizes 1 (500 Units)
Catalog IDs RV1116
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XbaI Applied Biological Materials
Enzyme Type Restriction enzyme Templates dsDNA Applicable Processes Molecular cloning
From $ 35.00 (500 µl)
Sizes 1 (500 µl)
Catalog IDs E052
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AflII Applied Biological Materials
Enzyme Type Restriction enzyme Templates dsDNA Applicable Processes Molecular cloning
From $ 135.00 (500 µl)
Sizes 1 (500 µl)
Catalog IDs E050
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T7 Endonuclease I Applied Biological Materials
Enzyme Type Endonuclease Templates dsDNA Applicable Processes Molecular cloning
From $ 125.00 (100 µl)
Sizes 1 (100 µl)
Catalog IDs E028
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EasyScript™ Reverse Transcriptase (PCR) Applied Biological Materials
Enzyme Type Reverse transcriptase Templates mRNA, ssRNA, Total RNA Applicable Processes cDNA synthesis, Library preparation, Sequencing
From $ 20.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs G231, G232
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GenCrispr Cas9-N-NLS Nuclease GenScript
Enzyme Type Nuclease Templates Plasmid DNA Applicable Processes CRISPR
From $ 635.00 (50 µg)
Sizes 2 (50 - 100 µg)
Catalog IDs Z03388-50, Z03388-100
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Long-Range DNA Polymerase (PCR) Applied Biological Materials
Enzyme Type Polymerase Templates Genomic DNA, Plasmid DNA, Viral DNA Applicable Processes End-point PCR
From $ 30.00 (250 Units)
Sizes 2 (250 - 1000 Units)
Catalog IDs G460, G461
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BauI (BssSI) (10 U/µL) Thermo Scientific
Enzyme Type Restriction enzyme Templates dsDNA Applicable Processes Enzymatic reaction
From (on request)
Sizes 1 (200 Units)
Catalog IDs ER1841
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BclI (10 U/µL) Thermo Scientific
Enzyme Type Restriction enzyme Templates dsDNA Applicable Processes Enzymatic reaction
From (on request)
Sizes 3 (1000 - 5000 Units)
Catalog IDs ER0721, ER0725, ER0722
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

The basics of enzymes

Enzymes lower the activation energy of a reaction, thus accelerating the reaction rate of the conversion of substrate to product. The increased rate can be by a factor of several million and without this increased reaction rate, life would not be able to be sustained here on Earth.
Most enzymes are proteins, but there are a few exceptions where RNA molecules can act as catalysts for reactions.
Since the enzyme is a catalyst, it is not consumed during the reaction. Thus, decreased reaction rates observed over time is not due to the enzyme concentration decreasing, but a combination of reduced availability of substrates and an accumulation of product.
Enzymes often work within very narrow pH and temperature ranges. Hence, it is essential that you use the correct buffers and optimal temperature ranges given in the protocol that is supplied with the enzyme.
Enzymes can lower the activation energy (ΔG‡, Gibbs free energy) of a reaction in several ways.

  • Stabilization of the transition state
  • Providing alternative pathways
  • Destabilization of the substrate ground state

The nomenclature of enzymes is determined by the substrate that is used in the reaction and with the addition of the suffix -ase. One example of this would be the alcohol-metabolizing enzyme alcohol dehydrogenase.
Generally, enzymes are divided into the following groups:

  • Oxidoreductases: catalyze oxidation/reduction reactions
  • Ligases: can join molecules with covalent bonds
  • Transferases: can transfer functional groups, such as polymerases
  • Isomerases: can catalyze isomerization changes
  • Lyases: cleave various bonds (not hydrolysis and oxidation)

Isozymes is the name given to enzymes that are different, but are catalyzing the same chemical reaction.

Our Restriction Enzyme Troubleshoot is useful for solving any potential problems.

Compare Enzymes

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the IPROOF™ HIGH-FIDELITY DNA POLYMERASE Kit from Bio-Rad accepts amplicons up to 37 kb.
  • that the TAQ PLUS DNA POLYMERASE Kit from G-Biosciences is the cheapest per unit.
  • that the PLATINUM™ SUPERFI™ GREEN DNA POLYMERASE Kit from Invitrogen is compatible with the widest range of templates.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.