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cDNA Synthesis Kits

OneScribe T7 Transcription Kit

Applied Biological Materials

From
$ 185.00 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
E081
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ORF Clones & Clone Collections

KRAS (NM_004985) Human Untagged Clone

Origene

From
$ 196.00 (10 µg)
Sizes
1 (10 µg)
Catalog IDs
SC109374
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cDNA Synthesis Kits

Score 0.80

OneScript Plus cDNA Synthesis SuperMix

Applied Biological Materials

Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
Transcriptase
EasyScript Plus™ Reverse Transcriptase
EasyScript Plus™ Reverse Transcriptase
From
$ 60.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
G453, G454
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cDNA Synthesis Kits

Score 0.80

5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)

Applied Biological Materials

Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
> 2 µg
> 2 µg
Transcriptase
EasyScript™ Reverse Transcriptase
EasyScript™ Reverse Transcriptase
From
$ 160.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
G492
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cDNA Synthesis Kits

miRNA cDNA Synthesis Kit

Applied Biological Materials

Templates
Total RNA, ssDNA
Total RNA, ssDNA
Template Amount
2 μg total RNA, 200 ng small RNA
2 μg total RNA, 200 ng small RNA
Transcriptase
EasyScript™ RT
EasyScript™ RT
From
$ 135.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
G269, G270
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cDNA Synthesis Kits

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based

Applied Biological Materials

Templates
cDNA
cDNA
Template Amount
10 ng - 2 µg first strand cDNA
10 ng - 2 µg first strand cDNA
Transcriptase
/
/
From
$ 455.00 (25 Reactions)
Sizes
1 (25 Reactions)
Catalog IDs
G476
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cDNA Synthesis Kits

µMACS One-step cDNA Kit

Miltenyi Biotec

From
$ 530.00 (20 Reactions)
Sizes
1 (20 Reactions)
Catalog IDs
130-091-902
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ORF Clones & Clone Collections

TransIT(R)-Keratinocyte Transfection Reagent

Mirus

Organism
/
/
Gene
/
/
Concentration
/
/
From
$ 256.50 (0.4 ml)
Sizes
2 (0.4 - 1 ml)
Catalog IDs
MIR 2804, MIR 2800
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ORF Clones & Clone Collections

TransIT(R)-Oligo Transfection Reagent

Mirus

Organism
/
/
Gene
/
/
Concentration
/
/
From
$ 263.00 (0.4 ml)
Sizes
2 (0.4 - 1 ml)
Catalog IDs
MIR 2164, MIR 2160
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ORF Clones & Clone Collections

VIVACON 2, 10K MWCO

Sartorius

Organism
/
/
Gene
/
/
Concentration
/
/
From
$ 22.90 (4 Units)
Sizes
4 (4 - 500 Units)
Catalog IDs
VN02H0S, VN02H01, ...
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ORF Clones & Clone Collections

VIVACON 2 30K MWCO

Sartorius

Organism
/
/
Gene
/
/
Concentration
/
/
From
$ 22.90 (4 Units)
Sizes
4 (4 - 500 Units)
Catalog IDs
VN02H2S, VN02H21, ...
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ORF Clones & Clone Collections

VIVACON 2 100K MWCO

Sartorius

Organism
/
/
Gene
/
/
Concentration
/
/
From
$ 22.90 (4 Units)
Sizes
4 (4 - 500 Units)
Catalog IDs
VN02H4S, VN02H41, ...
Added to comparison remove item

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The Genome

A genome - the entire genetic material present in an organism - can be anywhere between 1.8 kb to 130 Gb base pairs long. So when studying the genetics of an organism, scientists like to break down their chosen genome of interest into manageable pieces. Once done, it is then possible to test and determine the DNA segment’s function.

To begin such experiments, clone collections are often used. Put simply; these are banks of genetically modified, easily transfected microbial communities. Individual microbes hold a segment of the target genome, and together, the microbial community collectively holds the entire genome. So a clone collection is an extensive ‘library’ of cDNA or genome clones from an organism of interest, like human, mouse, or rat, in select expression vectors.

Clone Collections

In building such clone collections, scientists utilize natural occurring processes to simulate DNA recombination. That is, in cloning, DNA is fragmented into smaller segments of whole genes, promoter sequences, non-coding sequences or random fragments via restriction enzymes. These are then ligated into desired DNA strands of target genes or sequences, forming recombinant DNA. Once the recombinant DNA is ready, it is inserted into a vector which is then transfected into a competent cell. Scientists can then screen colonies for those that were successfully transfected, usually with a white-blue test.

cDNA Collection

cDNA collections only contain active genes . Since cDNA is made from a reverse transcriptase enzyme, which converts mRNA back into DNA, the collection will contain only the genes which were transcribed by DNA.

A clone collection is a large source of genetic material of a target organism, so scientists may perform more accurate tests, ranging from genetic fingerprinting to protein production.

Vector Selection

Clone collections usually come in a variety of clone vectors, each with a range of insert sizes. For example, a plasmid has space to insert up to 15k base pairs, whereas Yeast Artificial Chromosomes (YACs) range from 250 to 2000k bp. Which vector is right for you depends on the size of the genome of your target organism.

Plasmid: a double-stranded circular DNA, independent from chromosomal DNA, and is found in most bacteria. Plasmids range from 2 – 4 kb in length and can carry inserts up to 15kb.

Phage lambda: a strand of viral DNA that infects E. coli. This virus is 48.5kb in length and can carry inserts up to 25kb. These inserts are joined with the viral DNA, which codes for the construction of viral particles. These then infect the host cell and then reformed with the help of local DNA ligase.

Cosmid: a hybrid of a plasmid vector and the Phage lambda. More specifically, a cosmid is a plasmid that contains the cos sequence from the Phage Lambda virus as described previously. This allows the cosmid to pack itself into viral particles, that once transduced into a host cell, the cosmid reassembles itself into a plasmid with the help of the DNA ligase. This mechanism allows cosmids to hold up to 45kb of inserts.

Bacteriophage P1 Vector: a linear DNA strand that can maintain inserts up to 70 – 100 kb in size. Similar to phage lambda and cosmids, Bacteriophage P1 vector DNA can package itself into particles and infects a host cell where the p1 vector then circularizes itself.

Bacterial Artificial Chromosome: BACs are circular DNA that is around 7 kb in length, capable of holding inserts up to 300 kb in size, and contain the crucial Fertility factor. This F-factor allows for the even distribution of plasmids after cell division, thus ensuring the transfer of your DNA insert.

Yeast Artificial Chromosome: YACs are linear DNA molecules of a normal yeast chromosome that can have inserts around 250 – 2000 kb in size.