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cDNA Synthesis System
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
1-20 µg total RNA, 0.3-2 µg mRNA
1-20 µg total RNA, 0.3-2 µg mRNA
Transcriptase
AMV RT
AMV RT
Scientific Score
6.26
Brand
Roche Life Science
Sizes
1 (10 Reactions)
Available From
1 Vendor
Price
$ 681.00
Catalog IDs
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TruScript First Strand cDNA Synthesis Kit for mRNA
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
1 pg - 1 µg total RNA or 1 pg - 500 ng mRNA
1 pg - 1 µg total RNA or 1 pg - 500 ng mRNA
Transcriptase
TruScript RT (modified M-MuLV RT)
TruScript RT (modified M-MuLV RT)
Scientific Score
5.52
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Vendor
Price
$ 324.00
Catalog IDs
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M-MLV REVERSE TRANSCRIPTASE
Templates
Total RNA
Total RNA
Template Amount
/
Transcriptase
M-MLV RT
M-MLV RT
Scientific Score
4.09
Brand
AMRESCO
Sizes
1 (200 Reactions)
Available From
2 Vendors
Price Range
$ 263.20 - $ 311.36
Catalog IDs
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TruScript First Strand cDNA Synthesis Kit
Templates
Total RNA
Total RNA
Template Amount
1 pg - 1 µg
1 pg - 1 µg
Transcriptase
TruScript RT (modified M-MuLV RT)
TruScript RT (modified M-MuLV RT)
Scientific Score
6.21
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Vendor
Price
$ 340.00
Catalog IDs
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SensiFAST cDNA Synthesis Kit
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
< 1 µg
< 1 µg
Transcriptase
RT
RT
Scientific Score
9.46
Brand
Bioline
Sizes
2 (50 - 250 Reactions)
Available From
1 Vendor
Price Range
$ 175.00 - $ 800.00
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OneScript cDNA Synthesis Kit (with AccuRT Genomic DNA Removal Kit)
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
> 2 µg
> 2 µg
Transcriptase
EasyScript™ RT
EasyScript™ RT
Scientific Score
8.72
Brand
Applied Biological Materials
Sizes
1 (100 Reactions)
Available From
1 Vendor
Price
$ 140.00
Catalog IDs
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OneScript cDNA Synthesis Kit
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
Transcriptase
OneScript™ Reverse Transcriptase
OneScript™ Reverse Transcriptase
Scientific Score
9.26
Brand
Applied Biological Materials
Sizes
2 (25 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 50.00 - $ 115.00
Catalog IDs
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5X All-In-One RT MasterMix (with ExCellenCT Lysis Kit)
Templates
Cells
Cells
Template Amount
10-10^5 cells
10-10^5 cells
Transcriptase
EasyScript™ Reverse Transcriptase
EasyScript™ Reverse Transcriptase
Scientific Score
8.97
Brand
Applied Biological Materials
Sizes
1 (25 Preparations)
Available From
1 Vendor
Price
$ 170.00
Catalog IDs
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5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
> 2 µg
> 2 µg
Transcriptase
EasyScript™ Reverse Transcriptase
EasyScript™ Reverse Transcriptase
Scientific Score
8.87
Brand
Applied Biological Materials
Sizes
1 (100 Reactions)
Available From
1 Vendor
Price
$ 160.00
Catalog IDs
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HotScriptase RT Polymerase
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
Recommended final template concentration: 5-500 ng/µl
Recommended final template concentration: 5-500 ng/µl
Transcriptase
HotScriptase RT Polymerase
HotScriptase RT Polymerase
Scientific Score
8.37
Brand
Genaxxon Bioscience
Sizes
2 (250 - 1000 Units)
Available From
1 Vendor
Price Range
$ 121.19 - $ 403.35
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AccuPower RT PreMix SKU: K-2043
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Template Amount
0.1-2 µg
0.1-2 µg
Transcriptase
MMLV Reverse Transcriptase
MMLV Reverse Transcriptase
Scientific Score
6.60
Brand
Bioneer
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 147.42
Catalog IDs
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AccuPower RT PreMix SKU: K-2261
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Template Amount
0.05-1 µg
0.05-1 µg
Transcriptase
MMLV Reverse Transcriptase
MMLV Reverse Transcriptase
Scientific Score
7.49
Brand
Bioneer
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 98.28
Catalog IDs
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cDNA Synthesis Outline & Application

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs). The resultant complementary DNA (cDNA) constitutes the starting material for molecular cloning, hybridization probes and the creation of clone libraries, but is also commonly used for transcriptome analysis in qPCR or microarray experiments. It is a necessary initial step for PCR of an RNA sample.

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Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

total RNA

mRNA

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • If you plan to use cDNA clones only for transcriptome analysis, you will need a first-strand synthesis kit

  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Video: abhchannel/YouTube

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