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illustra Ready-To-Go RT-PCR Beads (0.2 ml Tubes)
Templates
/
Template Amount
/
Transcriptase
/
Scientific Score
3.23
Brand
GE Healthcare Life Sciences
Sizes
1 (1 Unit)
Available From
2 Vendors
Price Range
$ 433.00 - $ 433.00
Catalog IDs
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illustra Ready-To-Go RT-PCR Beads (0.2 ml Hinged Tube with Cap)
Templates
/
Template Amount
/
Transcriptase
/
Scientific Score
3.23
Brand
GE Healthcare Life Sciences
Sizes
1 (1 Unit)
Available From
2 Vendors
Price Range
$ 431.00 - $ 465.66
Catalog IDs
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OneScribe T7 Transcription Kit
Templates
/
Template Amount
/
Transcriptase
/
Scientific Score
2.27
Brand
Applied Biological Materials
Sizes
1 (50 Reactions)
Available From
1 Vendor
Price
$ 185.00
Catalog IDs
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Total-Transcriptome cDNA Synthesis Kit
Templates
/
Template Amount
/
Transcriptase
/
Scientific Score
2.41
Brand
Applied Biological Materials
Sizes
2 (25 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 185.00 - $ 525.00
Catalog IDs
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TeloPrime Full-Length cDNA Amplification Kit
Templates
/
Template Amount
/
Transcriptase
/
Scientific Score
1.28
Brand
Lipocalyx
Sizes
2 (8 - 24 Reactions)
Available From
1 Vendor
Price Range
$ 550.00 - $ 1,250.00
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OneScript Plus cDNA Synthesis Kit
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
Transcriptase
OneScript® Plus Reverse Transcriptase
OneScript® Plus Reverse Transcriptase
Scientific Score
6.36
Brand
Applied Biological Materials
Sizes
2 (25 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 60.00 - $ 150.00
Catalog IDs
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Second-Strand cDNA Synthesis Kit-dNTP/dUTP based
Templates
cDNA
cDNA
Template Amount
10 ng - 2 µg first strand cDNA
10 ng - 2 µg first strand cDNA
Transcriptase
/
Scientific Score
7.24
Brand
Applied Biological Materials
Sizes
1 (25 Reactions)
Available From
1 Vendor
Price
$ 455.00
Catalog IDs
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Second Strand cDNA Synthesis Kit-dNTP based
Templates
cDNA
cDNA
Template Amount
10 ng - 2 µg first strand cDNA
10 ng - 2 µg first strand cDNA
Transcriptase
/
Scientific Score
7.44
Brand
Applied Biological Materials
Sizes
1 (25 Reactions)
Available From
1 Vendor
Price
$ 360.00
Catalog IDs
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miRNA cDNA Synthesis Kit, with Poly(A) Polymerase Tailing
Templates
Total RNA
Total RNA
Template Amount
2 μg total RNA, 200 ng small RNA
2 μg total RNA, 200 ng small RNA
Transcriptase
EasyScript™ RT
EasyScript™ RT
Scientific Score
8.82
Brand
Applied Biological Materials
Sizes
2 (25 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 160.00 - $ 550.00
Catalog IDs
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miRNA cDNA Synthesis Kit
Templates
Total RNA, ssDNA
Total RNA, ssDNA
Template Amount
2 μg total RNA, 200 ng small RNA
2 μg total RNA, 200 ng small RNA
Transcriptase
EasyScript™ RT
EasyScript™ RT
Scientific Score
8.92
Brand
Applied Biological Materials
Sizes
2 (25 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 90.00 - $ 260.00
Catalog IDs
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HotScriptase RT Cell Master Mix
Templates
Cells, Total RNA, Viral RNA, mRNA
Cells, Total RNA, Viral RNA, mRNA
Template Amount
Recommended final template concentration: 5-500 ng/µl
Recommended final template concentration: 5-500 ng/µl
Transcriptase
HotScriptase RT Polymerase
HotScriptase RT Polymerase
Scientific Score
8.18
Brand
Genaxxon Bioscience
Sizes
2 (1250 - 6250 µl)
Available From
1 Vendor
Price Range
$ 242.39 - $ 1,048.47
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HotScriptase RT Master Mix Probe without ROX
Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
Recommended final template concentration: 5-500 ng/µl
Recommended final template concentration: 5-500 ng/µl
Transcriptase
HotScriptase RT Polymerase
HotScriptase RT Polymerase
Scientific Score
8.08
Brand
Genaxxon Bioscience
Sizes
2 (1250 - 6250 µl)
Available From
1 Vendor
Price Range
$ 201.99 - $ 873.83
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cDNA Synthesis Outline & Application

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs). The resultant complementary DNA (cDNA) constitutes the starting material for molecular cloning, hybridization probes and the creation of clone libraries, but is also commonly used for transcriptome analysis in qPCR or microarray experiments. It is a necessary initial step for PCR of an RNA sample.

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Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

total RNA

mRNA

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • If you plan to use cDNA clones only for transcriptome analysis, you will need a first-strand synthesis kit

  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Video: abhchannel/YouTube

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