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cDNA Synthesis Kits
TransScript® -Uni Cell to cDNA Synthesis SuperMix for qPCR
Templates
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Template Amount
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Transcriptase
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Scientific Score
2.22
Brand
TransGen Biotech
Sizes
1 (25 Reactions)
Available From
1 Vendor
Price
$ 307.69
Catalog IDs
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cDNA Synthesis Kits
EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix
Templates
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Template Amount
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Transcriptase
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Scientific Score
2.36
Brand
TransGen Biotech
Sizes
2 (50 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 107.69 - $ 193.85
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cDNA Synthesis Kits
TransScript® II All-in-One First-Strand cDNA Synthesis SuperMix for PCR
Templates
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Template Amount
/
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Transcriptase
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Scientific Score
2.02
Brand
TransGen Biotech
Sizes
1 (50 Reactions)
Available From
1 Vendor
Price
$ 338.46
Catalog IDs
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cDNA Synthesis Kits
OneScribe T7 Transcription Kit
Scientific Score
2.27
Brand
Applied Biological Materials
Sizes
1 (50 Reactions)
Available From
1 Vendor
Price
$ 185.00
Catalog IDs
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cDNA Synthesis Kits
AccuPower RT PreMix SKU: K-2261
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Template Amount
0.05-1 µg
0.05-1 µg
Transcriptase
MMLV Reverse Transcriptase
MMLV Reverse Transcriptase
Scientific Score
7.49
Brand
Bioneer
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 98.28
Catalog IDs
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cDNA Synthesis Kits
AccuPower CycleScript RT PreMix dT20 SKU: K-2047
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Template Amount
0.01-1 µg
0.01-1 µg
Transcriptase
CycleScript Reverse Transcriptase
CycleScript Reverse Transcriptase
Scientific Score
7.73
Brand
Bioneer
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 224.91
Catalog IDs
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cDNA Synthesis Kits
AccuPower RocketScript RT PreMix SKU: K-2101
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Template Amount
10 pg - 5 µg
10 pg - 5 µg
Transcriptase
RocketScript Reverse Transcriptase
RocketScript Reverse Transcriptase
Scientific Score
8.03
Brand
Bioneer
Sizes
2 (96 - 480 Reactions)
Available From
1 Vendor
Price Range
$ 183.33 - $ 686.07
Catalog IDs
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cDNA Synthesis Kits
AccuPower RocketScript RT PreMix SKU: K-2103
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Template Amount
10 pg - 5 µg
10 pg - 5 µg
Transcriptase
RocketScript Reverse Transcriptase
RocketScript Reverse Transcriptase
Scientific Score
7.17
Brand
Bioneer
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 274.05
Catalog IDs
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cDNA Synthesis Kits
AccuPower RocketScriptCycleRT PreMix dT20 SKU: K-2203
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Template Amount
10 pg - 5 µg
10 pg - 5 µg
Transcriptase
RocketScript Reverse Transcriptase
RocketScript Reverse Transcriptase
Scientific Score
7.17
Brand
Bioneer
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 344.97
Catalog IDs
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cDNA Synthesis Kits
MystiCq® microRNA cDNA Synthesis Mix
Templates
Total RNA, miRNA
Total RNA, miRNA
Template Amount
< 1 µg
< 1 µg
Transcriptase
ReadyScript RT
ReadyScript RT
Scientific Score
7.54
Brand
Sigma-Aldrich
Sizes
2 (25 - 100 Reactions)
Available From
2 Vendors
Price Range
$ 273.00 - $ 913.00
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cDNA Synthesis Kits
AMPIGENE® cDNA Synthesis Kit
Templates
Total RNA
Total RNA
Template Amount
4 pg - 4 µg
4 pg - 4 µg
Transcriptase
modified M-MLV RT
modified M-MLV RT
Scientific Score
6.90
Brand
Enzo Life Sciences
Sizes
2 (50 - 200 Reactions)
Available From
1 Vendor
Price Range
$ 205.00 - $ 738.00
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cDNA Synthesis Kits
qScript cDNA Synthesis Kit
Templates
Total RNA
Total RNA
Template Amount
10 pg - 4 µg
10 pg - 4 µg
Transcriptase
qScript RT
qScript RT
Scientific Score
9.06
Brand
Quantabio
Sizes
3 (25 - 500 Reactions)
Available From
1 Vendor
Price Range
$ 106.00 - $ 1,070.00
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cDNA Synthesis Outline & Application

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs). The resultant complementary DNA (cDNA) constitutes the starting material for molecular cloning, hybridization probes and the creation of clone libraries, but is also commonly used for transcriptome analysis in qPCR or microarray experiments. It is a necessary initial step for PCR of an RNA sample.

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Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

total RNA

mRNA

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • If you plan to use cDNA clones only for transcriptome analysis, you will need a first-strand synthesis kit

  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Video: abhchannel/YouTube

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