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cDNA Synthesis Kits on ZAGENO

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based Applied Biological Materials
Templates cDNA Template Amount 10 ng - 2 µg first strand cDNA Time to Sample 150 min
From $ 455.00 (25 Reactions)
Sizes 1 (25 Reactions)
Catalog IDs G476
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AMPIGENE® cDNA Synthesis Kit Enzo Life Sciences
Templates Total RNA Template Amount 4 pg - 4 µg Time to Sample > 40 min
From $ 205.00 (50 Reactions)
Sizes 2 (50 - 200 Reactions)
Catalog IDs ENZ-KIT106-0050, ENZ-KIT106-0200
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MystiCq® microRNA cDNA Synthesis Mix Sigma-Aldrich
Templates Total RNA, miRNA Template Amount < 1 µg Time to Sample > 90 min (Poly (A) Tailing reaction and first-strand cDNA synthesis)
From $ 265.50 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs MIRRT-25RXN, MIRRT-100RXN
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JetSeq DNA Library Preparation Kit Bioline
Templates / Template Amount / Time to Sample < 180 min
From $ 720.00 (16 Reactions)
Sizes 1 (16 Reactions)
Catalog IDs BIO-68025
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OneScript Plus cDNA Synthesis SuperMix Applied Biological Materials
Templates mRNA, Total RNA Template Amount 1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA Time to Sample 30 min for qPCR, 1 hour for PCR
From $ 60.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs G453, G454
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miRNA cDNA Synthesis Kit Applied Biological Materials
Templates ssDNA, Total RNA Template Amount 2 μg total RNA, 200 ng small RNA Time to Sample 30-45 min
From $ 90.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs G269, G270
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HotScriptase RT Master Mix Probe without ROX Genaxxon Bioscience
Templates Total RNA, mRNA Template Amount Recommended final template concentration: 5-500 ng/µl Time to Sample 27-62 min
From $ 177.00 (1.25 ml)
Sizes 2 (1.25 - 6.25 ml)
Catalog IDs M3064.0050, M3064.0250
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M-MLV Reverse Transcriptase (200 U/uL) Invitrogen
Templates mRNA, Total RNA Template Amount 1 ng-5 µg total RNA, 1-500 ng mRNA Time to Sample 92 min
From $ 246.00 (40000 Units)
Sizes 2 (40000 - 200000 Units)
Catalog IDs 28025013, 28025021
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Maxima H Minus Reverse Transcriptase (200 U/uL) Thermo Scientific
Templates mRNA, Total RNA Template Amount 1 pg - 5 µg total RNA, 0.1 pg - 500 ng mRNA, 0.01 pg - 500 ng specific RNA Time to Sample > 20 min
From $ 58.75 (2000 Units)
Sizes 3 (2000 - 40000 Units)
Catalog IDs EP0751, EP0752, ...
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RevertAid H Minus Reverse Transcriptase (200 U/uL) Thermo Scientific
Templates mRNA, Total RNA Template Amount 0.1 ng-5 µg total RNA, 10 pg-500 ng poly(A) RNA, 0.01 pg-0.5 µg specific RNA Time to Sample 85 min
From $ 106.00 (10000 Units)
Sizes 2 (10000 - 50000 Units)
Catalog IDs EP0451, EP0452
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SuperScript® II Reverse Transcriptase Invitrogen
Templates mRNA, Total RNA Template Amount 1 ng–5 μg total RNA, 1–500 ng mRNA Time to Sample 75 min
From $ 91.25 (2000 Units)
Sizes 3 (2000 - 40000 Units)
Catalog IDs 18064022, 18064014, ...
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cDNA Synthesis Outline & Application

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs). The resultant complementary DNA (cDNA) constitutes the starting material for molecular cloning, hybridization probes and the creation of clone libraries, but is also commonly used for transcriptome analysis in qPCR or microarray experiments. It is a necessary initial step for PCR of an RNA sample.

Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • If you plan to use cDNA clones only for transcriptome analysis, you will need a first-strand synthesis kit
  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video: abhchannel/YouTube

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