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cDNA Synthesis Kits

OneScribe T7 Transcription Kit

Applied Biological Materials

From
$ 185.00 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
E081
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cDNA Synthesis Kits

Score 0.80

OneScript Plus cDNA Synthesis SuperMix

Applied Biological Materials

Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
1 ng - 2 µg total RNA, 1 pg - 2 ng Poly(A) + mRNA
Transcriptase
EasyScript Plus™ Reverse Transcriptase
EasyScript Plus™ Reverse Transcriptase
From
$ 60.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
G453, G454
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cDNA Synthesis Kits

Score 0.80

5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)

Applied Biological Materials

Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
> 2 µg
> 2 µg
Transcriptase
EasyScript™ Reverse Transcriptase
EasyScript™ Reverse Transcriptase
From
$ 160.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
G492
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cDNA Synthesis Kits

miRNA cDNA Synthesis Kit

Applied Biological Materials

Templates
Total RNA, ssDNA
Total RNA, ssDNA
Template Amount
2 μg total RNA, 200 ng small RNA
2 μg total RNA, 200 ng small RNA
Transcriptase
EasyScript™ RT
EasyScript™ RT
From
$ 135.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
G269, G270
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cDNA Synthesis Kits

Second-Strand cDNA Synthesis Kit-dNTP/dUTP based

Applied Biological Materials

Templates
cDNA
cDNA
Template Amount
10 ng - 2 µg first strand cDNA
10 ng - 2 µg first strand cDNA
Transcriptase
/
/
From
$ 455.00 (25 Reactions)
Sizes
1 (25 Reactions)
Catalog IDs
G476
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cDNA Synthesis Kits

µMACS One-step cDNA Kit

Miltenyi Biotec

From
$ 530.00 (20 Reactions)
Sizes
1 (20 Reactions)
Catalog IDs
130-091-902
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cDNA Synthesis Kits

Score 5.40

qScript cDNA Synthesis Kit

Quantabio

Templates
Total RNA
Total RNA
Template Amount
10 pg - 4 µg
10 pg - 4 µg
Transcriptase
qScript RT
qScript RT
From
$ 106.00 (25 Reactions)
Sizes
3 (25 - 500 Reactions)
Catalog IDs
95047-025, 95047-100, ...
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cDNA Synthesis Kits

HotScriptase RT Polymerase

Genaxxon Bioscience

Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
Recommended final template concentration: 5-500 ng/µl
Recommended final template concentration: 5-500 ng/µl
Transcriptase
HotScriptase RT Polymerase
HotScriptase RT Polymerase
From
$ 111.51 (250 Units)
Sizes
2 (250 - 1000 Units)
Catalog IDs
M3056.0250, M3056.1000
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cDNA Synthesis Kits

HotScriptase RT Master Mix Probe without ROX

Genaxxon Bioscience

Templates
Total RNA, mRNA
Total RNA, mRNA
Template Amount
Recommended final template concentration: 5-500 ng/µl
Recommended final template concentration: 5-500 ng/µl
Transcriptase
HotScriptase RT Polymerase
HotScriptase RT Polymerase
From
$ 177.00 (1.25 ml)
Sizes
2 (1.25 - 6.25 ml)
Catalog IDs
M3064.0050, M3064.0250
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cDNA Synthesis Kits

Score 5.00

MystiCq® microRNA cDNA Synthesis Mix

Sigma-Aldrich

Templates
Total RNA, miRNA
Total RNA, miRNA
Template Amount
< 1 µg
< 1 µg
Transcriptase
ReadyScript RT
ReadyScript RT
From
$ 273.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
MIRRT-25RXN, MIRRT-100RXN
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cDNA Synthesis Kits

TransScript® -Uni Cell to cDNA Synthesis SuperMix for qPCR

TransGen Biotech

Templates
/
/
Template Amount
/
/
Transcriptase
/
/
From
$ 307.69 (25 Reactions)
Sizes
1 (25 Reactions)
Catalog IDs
AC301-01
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cDNA Synthesis Kits

EasyScript® One-Step gDNA Removal and cDNA Synthesis SuperMix

TransGen Biotech

Templates
/
/
Template Amount
/
/
Transcriptase
/
/
From
$ 107.69 (50 Reactions)
Sizes
2 (50 - 100 Reactions)
Catalog IDs
AE311-02, AE311-03
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cDNA Synthesis Outline & Application

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs). The resultant complementary DNA (cDNA) constitutes the starting material for molecular cloning, hybridization probes and the creation of clone libraries, but is also commonly used for transcriptome analysis in qPCR or microarray experiments. It is a necessary initial step for PCR of an RNA sample.

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Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

total RNA

mRNA

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • If you plan to use cDNA clones only for transcriptome analysis, you will need a first-strand synthesis kit

  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Video: abhchannel/YouTube

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