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cDNA Synthesis Kits on ZAGENO

MystiCq® microRNA cDNA Synthesis Mix Sigma-Aldrich
Templates Total RNA, miRNA
From $ 273.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs MIRRT-25RXN, MIRRT-100RXN
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AMPIGENE® cDNA Synthesis Kit Enzo Life Sciences
Templates Total RNA
From $ 205.00 (50 Reactions)
Sizes 2 (50 - 200 Reactions)
Catalog IDs ENZ-KIT106-0050, ENZ-KIT106-0200
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JetSeq DNA Library Preparation Kit Bioline
Templates /
From $ 720.00 (16 Reactions)
Sizes 1 (16 Reactions)
Catalog IDs BIO-68025
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M-MLV Reverse Transcriptase (200 U/uL) Invitrogen
Templates Total RNA, mRNA
From $ 253.00 (40000 Units)
Sizes 2 (40000 - 200000 Units)
Catalog IDs 28025013, 28025021
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qScript cDNA Synthesis Kit Quantabio
Templates Total RNA
From $ 106.00 (25 Reactions)
Sizes 3 (25 - 500 Reactions)
Catalog IDs 95047-025, 95047-100, ...
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OneScript Plus cDNA Synthesis SuperMix Applied Biological Materials
Templates Total RNA, mRNA
From $ 60.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs G453, G454
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5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) Applied Biological Materials
Templates Total RNA, mRNA
From $ 160.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs G492
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HotScriptase RT Polymerase Genaxxon Bioscience
Templates Total RNA, mRNA
From $ 111.51 (250 Units)
Sizes 2 (250 - 1000 Units)
Catalog IDs M3056.0250, M3056.1000
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HotScriptase RT Master Mix Probe without ROX Genaxxon Bioscience
Templates Total RNA, mRNA
From $ 177.00 (1.25 ml)
Sizes 2 (1.25 - 6.25 ml)
Catalog IDs M3064.0050, M3064.0250
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miRNA cDNA Synthesis Kit Applied Biological Materials
Templates Total RNA, ssDNA
From $ 135.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs G269, G270
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Second-Strand cDNA Synthesis Kit-dNTP/dUTP based Applied Biological Materials
Templates cDNA
From $ 455.00 (25 Reactions)
Sizes 1 (25 Reactions)
Catalog IDs G476
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AccuPower RT PreMix SKU: K-2261 Bioneer
Templates Total RNA, Viral RNA, mRNA
From $ 98.28 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-2261-1
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cDNA Synthesis Outline & Application

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs). The resultant complementary DNA (cDNA) constitutes the starting material for molecular cloning, hybridization probes and the creation of clone libraries, but is also commonly used for transcriptome analysis in qPCR or microarray experiments. It is a necessary initial step for PCR of an RNA sample.

Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • If you plan to use cDNA clones only for transcriptome analysis, you will need a first-strand synthesis kit
  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video: abhchannel/YouTube

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