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cDNA Synthesis Kits

Score 5.00

MystiCq® microRNA cDNA Synthesis Mix

Sigma-Aldrich

Templates
Total RNA, miRNA
Total RNA, miRNA
From
$ 273.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
MIRRT-25RXN, MIRRT-100RXN
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cDNA Synthesis Kits

AMPIGENE® cDNA Synthesis Kit

Enzo Life Sciences

Templates
Total RNA
Total RNA
From
$ 205.00 (50 Reactions)
Sizes
2 (50 - 200 Reactions)
Catalog IDs
ENZ-KIT106-0050, ENZ-KIT106-0200
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cDNA Synthesis Kits

Score 10.00

SuperScript® II Reverse Transcriptase

Invitrogen

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 94.00 (2000 Units)
Sizes
3 (2000 - 40000 Units)
Catalog IDs
18064022, 18064014, ...
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cDNA Synthesis Kits

Score 0.80

M-MLV Reverse Transcriptase (200 U/uL)

Invitrogen

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 253.00 (40000 Units)
Sizes
2 (40000 - 200000 Units)
Catalog IDs
28025013, 28025021
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cDNA Synthesis Kits

Maxima H Minus Reverse Transcriptase (200 U/uL)

Thermo Scientific

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 60.25 (2000 Units)
Sizes
3 (2000 - 40000 Units)
Catalog IDs
EP0751, EP0752, ...
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cDNA Synthesis Kits

RevertAid H Minus Reverse Transcriptase (200 U/uL)

Thermo Scientific

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 109.00 (10000 Units)
Sizes
2 (10000 - 50000 Units)
Catalog IDs
EP0451, EP0452
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cDNA Synthesis Kits

Score 5.40

qScript cDNA Synthesis Kit

Quantabio

Templates
Total RNA
Total RNA
From
$ 106.00 (25 Reactions)
Sizes
3 (25 - 500 Reactions)
Catalog IDs
95047-025, 95047-100, ...
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cDNA Synthesis Kits

Score 0.80

OneScript Plus cDNA Synthesis SuperMix

Applied Biological Materials

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 60.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
G453, G454
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cDNA Synthesis Kits

Score 0.80

5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit)

Applied Biological Materials

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 160.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
G492
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cDNA Synthesis Kits

HotScriptase RT Polymerase

Genaxxon Bioscience

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 111.51 (250 Units)
Sizes
2 (250 - 1000 Units)
Catalog IDs
M3056.0250, M3056.1000
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cDNA Synthesis Kits

HotScriptase RT Master Mix Probe without ROX

Genaxxon Bioscience

Templates
Total RNA, mRNA
Total RNA, mRNA
From
$ 177.00 (1.25 ml)
Sizes
2 (1.25 - 6.25 ml)
Catalog IDs
M3064.0050, M3064.0250
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cDNA Synthesis Kits

miRNA cDNA Synthesis Kit

Applied Biological Materials

Templates
Total RNA, ssDNA
Total RNA, ssDNA
From
$ 135.00 (25 Reactions)
Sizes
2 (25 - 100 Reactions)
Catalog IDs
G269, G270
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cDNA Synthesis Outline & Application

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs). The resultant complementary DNA (cDNA) constitutes the starting material for molecular cloning, hybridization probes and the creation of clone libraries, but is also commonly used for transcriptome analysis in qPCR or microarray experiments. It is a necessary initial step for PCR of an RNA sample.

Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • If you plan to use cDNA clones only for transcriptome analysis, you will need a first-strand synthesis kit
  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video: abhchannel/YouTube

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