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Molecular Cloning

Score 1.30

pSpark® III Blunt-end DNA Cloning Kit

Canvax Biotech

From
$ 179.00 (21 Reactions)
Sizes
1 (21 Reactions)
Catalog IDs
C0003
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Molecular Cloning

Score 1.39

pSpark® IV Blunt-end DNA Cloning Kit

Canvax Biotech

From
$ 179.00 (22 Reactions)
Sizes
1 (22 Reactions)
Catalog IDs
C0004
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Cloning

Score 2.50

ClonaCell™-HY Hybridoma Kit

STEMCELL Technologies

From
$ 983.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
03800
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Molecular Cloning

Score 1.95

Proligation-Free Cloning Kit

Applied Biological Materials

From
$ 100.00 (20 Reactions)
Sizes
2 (20 - 50 Reactions)
Catalog IDs
E086, E087
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Competent Cells

Score 3.52

BigEasy®-TSA™ Electrocompetent Cells

VWR International

Competence
Electro
Electro
Antibiotic Resistance
/
/
Bacterial Strain
/
/
From
$ 753.72 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
95024-146
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Molecular Cloning

Score 3.31

StrataClone Blunt PCR Cloning Kit, 20 rxns

Agilent

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 359.00 (20 Reactions)
Sizes
1 (20 Reactions)
Catalog IDs
240207
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Molecular Cloning

Score 1.94

DNA LIGATION KIT

Sigma-Aldrich

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 420.24 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
LIG1-1KT
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Molecular Cloning

Score 3.80

Cloneez Pcr Cloning Kit

GenScript

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 175.00 (24 Reactions)
Sizes
1 (24 Reactions)
Catalog IDs
L00339
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Molecular Cloning

Score 2.57

Clontech Labs 3P IN-FUSION HD CLONING + 10REA

Clontech

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 287.83 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
NC0465914
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Competent Cells

Score 1.36

Endura™ Competent Cells SKU : 71003-038

VWR International

From
$ 474.32 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
71003-038
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Competent Cells

Score 6.60

Novagen™ B834pLysS Competent Cells, SKU: 69042

MilliporeSigma

Competence
/
/
Antibiotic Resistance
/
/
Bacterial Strain
/
/
From
$ 130.00 (0.4 ml)
Sizes
2 (0.4 - 1 ml)
Catalog IDs
69042-3, 69042-4
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Competent Cells

Score 6.48

Novagen™ BLR(DE3) Competent Cells, SKU: 69053

MilliporeSigma

Competence
/
/
Antibiotic Resistance
/
/
Bacterial Strain
/
/
From
$ 108.00 (0.4 ml)
Sizes
2 (0.4 - 1 ml)
Catalog IDs
69053-3, 69053-4
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What is Cloning?

Cloning, in biological terms, generally refers to the process of creating genetically identical copies of an individual or organism through either the natural process of asexual reproduction or in the unnatural setting of a lab using tools capable of genetic modification. In biotechnology, these tools can be used to create cellular clones (cell cloning) or clones of biomolecules through a process called molecular cloning.

Molecular Cloning

In molecular cloning, enzymes, plasmid constructs and competent cells are used to amplify, or in other words, create multiple copies of a DNA sequence. These DNA copies can be whole gene sequences, non-coding sequences, transcriptional sites or simply short DNA fragments.

The stages involved in a typical DNA cloning procedure are as follows:

  • The first step involves the insertion of your isolated DNA sequence of interest into a circular piece of DNA known as a plasmid vector. DNA ligases and restriction enzymes enable this insertion to happen in a ‘cut and paste’ type process.
  • Once the DNA fragment of interest has successfully ligated into the construct, this new recombinant plasmid is then transfected into a cellular host (competent cell) by either a number of different transformation processes.
  • The transformed cells are then grown in specialized media to significantly increase their number. As these transformed cells reproduce they replicate the recombinant plasmid and pass it on to their daughter cells making copies or clones of the DNA that it contains.

If you are interested in purchasing the necessary tools required for DNA cloning have a look at the many products we have on offer in Molecular Cloning.

Competent Cells

In order for a cell to accept a recombinant plasmid, it needs to be competent. Some bacteria possess intrinsic competence meaning they naturally contain the machinery necessary to bring extracellular DNA across their cell membrane. These include some strains of Bacillus subtilis, Escherichia Coli and various strains of yeast. When a cell host is not naturally competent artificial competence can be induced by introducing conditions that do not naturally occur in nature. Exposing cells to calcium chloride, heat shock and other stressful conditions can promote competence in many cases. Other common techniques include electroporation, sonication and viral transfection.

The type of cell used during the transformation process will depend heavily on a multitude of factors including whether the DNA is to be expressed as a protein, the type of plasmid vector used and the overall end application of the cloning experiment.

Here at ZAGENO you have the unique possibility to compare competent cells from different suppliers to find the most suitable one for your cloning experiment!