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Molecular Cloning

Proligation-Free Cloning Kit

Applied Biological Materials

From
$ 100.00 (20 Reactions)
Sizes
2 (20 - 50 Reactions)
Catalog IDs
E086, E087
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Molecular Cloning

Cloneez Pcr Cloning Kit

GenScript

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 175.00 (24 Reactions)
Sizes
1 (24 Reactions)
Catalog IDs
L00339
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Molecular Cloning

pEASY®-T1 Cloning Kit

TransGen Biotech

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 92.31 (20 Reactions)
Sizes
2 (20 - 60 Reactions)
Catalog IDs
CT101-01, CT101-02
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Molecular Cloning

pEASY®-T1 Simple Cloning Kit

TransGen Biotech

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 92.31 (20 Reactions)
Sizes
2 (20 - 60 Reactions)
Catalog IDs
CT111-01, CT111-02
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Molecular Cloning

Score 10.00

StrataClone Blunt PCR Cloning Kit, 20 rxns

Agilent

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 359.00 (20 Reactions)
Sizes
1 (20 Reactions)
Catalog IDs
240207
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Molecular Cloning

TaKaRa DNA Ligation Kit LONG

Clontech

Cloning Type
Ligation
Ligation
Insert Type
Blunt end DNA, Sticky/cohesive end DNA
Blunt end DNA, Sticky/cohesive end DNA
Cloning Time
3-15 h
3-15 h
From
$ 191.00 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
6024
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Molecular Cloning

DNA Blunting Kit

Clontech

Cloning Type
Ligation
Ligation
Insert Type
Blunt end DNA
Blunt end DNA
Cloning Time
> 30 min
> 30 min
From
$ 206.00 (20 Reactions)
Sizes
1 (20 Reactions)
Catalog IDs
6025
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Molecular Cloning

Score 0.80

DNA Ligation Kit, Version 1

Clontech

Cloning Type
Ligation
Ligation
Insert Type
DNA
DNA
Cloning Time
> 3 min
> 3 min
From
$ 187.00 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
6021
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Molecular Cloning

Clontech Labs 3P IN-FUSION HD CLONING + 10REA

Clontech

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 287.83 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
NC0465914
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Molecular Cloning

Score 0.80

DNA Ligation Kit, Version 2.1

Clontech

Cloning Type
Ligation
Ligation
Insert Type
DNA
DNA
Cloning Time
> 3 min
> 3 min
From
$ 187.00 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
6022
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Molecular Cloning

DNA LIGATION KIT

Sigma-Aldrich

Cloning Type
/
/
Insert Type
/
/
Cloning Time
/
/
From
$ 420.24 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
LIG1-1KT
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Molecular Cloning

pSpark® III Blunt-end DNA Cloning Kit

Canvax Biotech

From
$ 179.00 (21 Reactions)
Sizes
1 (21 Reactions)
Catalog IDs
C0003
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Cloning Applications

Molecular cloning allows for the assembling of recombinant DNA in a vector, typically a plasmid vector, to express target genes in a host organism. Molecular cloning is the first step in virtually any molecular biology experiment, including PCR, mutant analysis, protein tagging and protein purification.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

The different cloning systems

Cloning kits provide ligase, plasmid vectors and all the reagent you need to start your cloning experiments. Cloning products are generally divided into the different categories based on the cloning methods used:

  • Classic ligation-based cloning

  • Gateway® (proprietary)

  • TOPO® systems (proprietary)

Ligation cloning is an efficient and cost-effective solution for standard cloning applications. It is based on the use of vectors containing multiple cloning sites: sections of the vector plasmid containing restriction enzyme cutting sites. This means that the choice of vector, for both expression and subcloning, will not only depend on your application but will also influence your PCR strategy, as you will need to insert compatible restriction enzyme endings on your amplified fragment.

Moreover, restriction digestions can create sticky (cohesive) and blunt ends: depending on the cloning strategy. Conveniently, there are dedicated kits for both blunt and sticky end ligations.

Whether you plan on cloning the same fragment into different expression vectors, fusing the same protein on different tags, or doing an extensive promoter swap experiment, it is a good idea to use a recombination-based system like Gateway® cloning. Gateway® is based on recombination rather than restriction and ligation and allows for the “swapping” of inserts between subcloning vectors (called pDONR vectors) and expression vectors. This method also allows for the combination of multiple donor vectors to create complex fusion proteins.

TOPO® cloning is based on a different concept but allows reusing vectors for multiple clones as in the Gateway system.

Any issues which arise during your experiment can be tackled with our Molecular Cloning Troubleshoot.

Video and Image Credits

Video: The University of Sydney/YouTube