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In-Fusion® HD Cloning Plus CE
Cloning Type
In-fusion cloning reaction
In-fusion cloning reaction
Insert Type
PCR product
PCR product
Cloning Time
> 15 min
> 15 min
Scientific Score
6.52
Brand
TaKaRa
Sizes
4 (10 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 231.00 - $ 1,521.00
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Brand
Lucigen
Sizes
1 (20 Reactions)
Available From
1 Vendor
Price
$ 1,035.00
Catalog IDs
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CloneSmart® HCAmp Blunt Cloning Kit
Scientific Score
0.44
Brand
Lucigen
Sizes
1 (20 Reactions)
Available From
1 Vendor
Price
$ 282.10
Catalog IDs
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CloneSmart® HCKan Blunt Cloning Kit
Scientific Score
0.44
Brand
Lucigen
Sizes
1 (20 Reactions)
Available From
1 Vendor
Price
$ 282.10
Catalog IDs
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CloneSmart® LCAmp Blunt Cloning Kit
Scientific Score
0.44
Brand
Lucigen
Sizes
1 (20 Reactions)
Available From
1 Vendor
Price
$ 334.21
Catalog IDs
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CloneSmart® LCKan Blunt Cloning Kit
Scientific Score
0.44
Brand
Lucigen
Sizes
1 (20 Reactions)
Available From
1 Vendor
Price
$ 334.21
Catalog IDs
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Brand
Lucigen
Sizes
1 (20 Reactions)
Available From
1 Vendor
Price
$ 988.00
Catalog IDs
Added to comparison remove item
Brand
Lucigen
Sizes
1 (20 Reactions)
Available From
1 Vendor
Price
$ 988.00
Catalog IDs
Added to comparison remove item
In-Fusion® HD Cloning Plus
Cloning Type
In-fusion cloning reaction
In-fusion cloning reaction
Insert Type
PCR product
PCR product
Cloning Time
> 15 min
> 15 min
Scientific Score
9.27
Brand
TaKaRa
Sizes
4 (10 - 100 Reactions)
Available From
1 Vendor
Price Range
$ 231.00 - $ 1,514.00
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StrataClone Blunt PCR Cloning Kit, 20 rxns
Cloning Type
/
Insert Type
/
Cloning Time
/
Scientific Score
3.31
Brand
Agilent
Sizes
1 (20 Reactions)
Available From
2 Vendors
Price Range
$ 359.00 - $ 613.53
Catalog IDs
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Brand
Canvax Biotech
Sizes
2 (10 - 20 Reactions)
Available From
1 Vendor
Price Range
$ 79.00 - $ 149.00
Catalog IDs
Added to comparison remove item
Brand
Canvax Biotech
Sizes
2 (10 - 20 Reactions)
Available From
1 Vendor
Price Range
$ 79.00 - $ 149.00
Catalog IDs
Added to comparison remove item

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Cloning Applications

Molecular cloning allows for the assembling of recombinant DNA in a vector, typically a plasmid vector, to express target genes in a host organism. Molecular cloning is the first step in virtually any molecular biology experiment, including PCR, mutant analysis, protein tagging and protein purification.

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The different cloning systems

Cloning kits provide ligase, plasmid vectors and all the reagent you need to start your cloning experiments. Cloning products are generally divided into the different categories based on the cloning methods used:

  • Classic ligation-based cloning

  • Gateway® (proprietary)

  • TOPO® systems (proprietary)

Ligation cloning is an efficient and cost-effective solution for standard cloning applications. It is based on the use of vectors containing multiple cloning sites: sections of the vector plasmid containing restriction enzyme cutting sites. This means that the choice of vector, for both expression and subcloning, will not only depend on your application but will also influence your PCR strategy, as you will need to insert compatible restriction enzyme endings on your amplified fragment.

Moreover, restriction digestions can create sticky (cohesive) and blunt ends: depending on the cloning strategy. Conveniently, there are dedicated kits for both blunt and sticky end ligations.

Whether you plan on cloning the same fragment into different expression vectors, fusing the same protein on different tags, or doing an extensive promoter swap experiment, it is a good idea to use a recombination-based system like Gateway® cloning. Gateway® is based on recombination rather than restriction and ligation and allows for the “swapping” of inserts between subcloning vectors (called pDONR vectors) and expression vectors. This method also allows for the combination of multiple donor vectors to create complex fusion proteins.

TOPO® cloning is based on a different concept but allows reusing vectors for multiple clones as in the Gateway system.

Any issues which arise during your experiment can be tackled with our Molecular Cloning Troubleshoot.

Video and Image Credits

Video: The University of Sydney/YouTube