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Electrophoresis Additives on ZAGENO

1 Kb DNA Ladders IBI Scientific
From $ 9.53 (2.5 µg)
Sizes 1 (2.5 µg)
Catalog IDs IB01350
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DNAmark™ 100bp Plus Ladder VWR International
From $ 70.36 (1 Unit)
Sizes 1 (1 Unit)
Catalog IDs 89233-914
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EconoTaq® PLUS GREEN 2X Master Mix VWR International
From $ 239.32 (1 Unit)
Sizes 1 (1 Unit)
Catalog IDs 95024-004
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IEF (Isoelectrofocusing) Buffers SKU: 10530003 bio-World
From $ 40.36 (500 ml)
Sizes 2 (1 - 500 L)
Catalog IDs 10530003-2, 10530003-1
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TAE Buffers SKU: 10530011 bio-World
From $ 28.23 (1 Pack)
Sizes 2 (1 - 4 Pack)
Catalog IDs 10530011-1, 10530011-2
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TAE Buffers SKU: 10530013 bio-World
From $ 67.23 (1 L)
Sizes 2 (1 - 4 L)
Catalog IDs 10530013-1, 10530013-2
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Crack-Free Gel-Drying Kit SKU: 10550018 bio-World
From $ 128.37 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs 10550018-1
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Wash solution, 10X SKU: 10550024 bio-World
From $ 23.60 (1000 ml)
Sizes 1 (1000 ml)
Catalog IDs 10550024-1
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Agarose Gel Dye SKU: 10570000 bio-World
From $ 27.15 (25 ml)
Sizes 2 (25 - 50 ml)
Catalog IDs 10570000-1, 10570000-2
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Agarose Gel Dye SKU: 10570001 bio-World
From $ 36.39 (25 ml)
Sizes 2 (25 - 50 ml)
Catalog IDs 10570001-1, 10570001-2
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Agarose Gel Dye SKU: 10570003 bio-World
From $ 31.80 (25 ml)
Sizes 2 (25 - 50 ml)
Catalog IDs 10570003-1, 10570003-2
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Laemmli SDS-Sample Buffer SKU: 10570018 bio-World
From $ 24.13 (25 ml)
Sizes 2 (25 - 50 ml)
Catalog IDs 10570018-1, 10570018-2
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Electrophoresis

Electrophoresis is the process by which DNA, RNA, and proteins are separated according to their size. Negatively charged nucleic acids or proteins migrate towards a positive electrode at a speed relative to their size, with smaller fragments traveling faster, and ultimately further. Various electrophoresis techniques are used in biological research. These include horizontal and vertical gel electrophoresis, agarose gel electrophoresis, polyacrylamide gel electrophoresis, and SDS-PAGE. Numerous additives are required to carry out electrophoresis successfully.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Standards

Molecular standards are essential in determining the approximate size of the macromolecules being analyzed. There are a wide range of DNA, RNA and protein ladders available in a range of sizes that can be used on either polyacrylamide or agarose gels.

Loading Dyes

Loading dyes and stains are both fundamental components of electrophoresis. Loading dyes are added to the nucleic acid or protein sample to increase the density of the sample and prevent it from diffusing into the buffer. Loading stains visualize the bands following electrophoresis, enabling analysis. The most commonly used loading stain for visualizing DNA/RNA is ethidium bromide, which fluoresces under UV light. Coomassie Brilliant Blue Dye is routinely used to visualize proteins.

Buffers

Buffers are another essential addition to an electrophoresis experiment. Buffers ensure that the pH remains stable so that proteins or nucleic acids are not denatured. Furthermore, buffers provide the necessary ions for maintaining a constant current. Frequently used buffers include TBE (tris-borate-EDTA) and TAE (Tris-acetate-EDTA). When selecting a buffer for your experiment, it is necessary to consider various properties of the buffer, such as the molecular size, formal charges, and pKa value.

Detergents

Detergents are crucial components of the electrophoretic separation of proteins. Detergents induce unfolding of proteins and provide a negatively-charged coating which enables migration and separation. Sodium dodecyl sulfate (SDS) is a commonly-used detergent that is available in a range of concentrations.