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Electrophoresis Additives

Score 4.96

TAE (10X)

Boston BioProducts

From
$ 20.00 (1 L)
Sizes
3 (1 - 4 L)
Catalog IDs
BM-250X-1L, BM-250X-2L, ...
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Electrophoresis Additives

Score 3.67

EZ-Ladder Molecular Weight Markers SKU: LB1

EZBiolab

From
$ 34.21 (2 Units)
Sizes
1 (2 Units)
Catalog IDs
LB1
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Electrophoresis Additives

Score 3.60

EZ-Ladder Molecular Weight Markers SKU: MKD003

EZBiolab

From
$ 43.79 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
MKD003
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Electrophoresis Additives

Score 6.37

Blue-Bandit Protein Stain

AMRESCO

From
$ 34.36 (1 ml)
Sizes
1 (1 ml)
Catalog IDs
K217-250ML
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Electrophoresis Additives

Score 6.41

MW Marker PCR Marker

AMRESCO

From
$ 116.59 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
E854-100RXN
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Electrophoresis Additives

Score 6.37

PCR Marker With Loading Dye

AMRESCO

From
$ 144.33 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
K811-50RXN
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Electrophoresis Additives

Score 4.07

DNA Markers SKU: ACT-IDMWD2

ACTGene

From
$ 86.62 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
ACT-IDMWD2
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Electrophoresis Additives

Score 8.93

2,4,6-Tris(2-pyridyl)-s-triazine SKU : T1253

Sigma-Aldrich

Reagent Type
/
/
Form
/
/
Applicable Processes
/
/
From
$ 67.00 (1 g)
Sizes
4 (1 - 25 g)
Catalog IDs
T1253-1G, T1253-5G, ...
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Electrophoresis Additives

Score 7.33

N,N′-Methylenebisacrylamide SKU : M7256

Sigma-Aldrich

From
$ 36.00 (25 g)
Sizes
3 (25 - 250 g)
Catalog IDs
M7256-25G, M7256-100G, ...
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Electrophoresis Additives

Score 6.91

Peptide Sequencing Standard SKU : P2046

Sigma-Aldrich

From
$ 47.60 (1 Vial)
Sizes
1 (1 Vial)
Catalog IDs
P2046-1VL
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Electrophoresis Additives

Score 6.98

PTH-α-aminoisobutyric acid SKU : P9001

Sigma-Aldrich

From
$ 87.30 (100 mg)
Sizes
1 (100 mg)
Catalog IDs
P9001-100MG
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Electrophoresis Additives

Score 8.74

TruPAGE™ Running Antioxidant SKU : PCG3007

Sigma-Aldrich

Reagent Type
/
/
Form
/
/
Applicable Processes
/
/
From
$ 34.00 (10 ml)
Sizes
1 (10 ml)
Catalog IDs
PCG3007-10ML
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Electrophoresis

Electrophoresis is the process by which DNA, RNA, and proteins are separated according to their size. Negatively charged nucleic acids or proteins migrate towards a positive electrode at a speed relative to their size, with smaller fragments traveling faster, and ultimately further. Various electrophoresis techniques are used in biological research. These include horizontal and vertical gel electrophoresis, agarose gel electrophoresis, polyacrylamide gel electrophoresis, and SDS-PAGE. Numerous additives are required to carry out electrophoresis successfully.

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Standards

Molecular standards are essential in determining the approximate size of the macromolecules being analyzed. There are a wide range of DNA, RNA and protein ladders available in a range of sizes that can be used on either polyacrylamide or agarose gels.

Loading Dyes

Loading dyes and stains are both fundamental components of electrophoresis. Loading dyes are added to the nucleic acid or protein sample to increase the density of the sample and prevent it from diffusing into the buffer. Loading stains visualize the bands following electrophoresis, enabling analysis. The most commonly used loading stain for visualizing DNA/RNA is ethidium bromide, which fluoresces under UV light. Coomassie Brilliant Blue Dye is routinely used to visualize proteins.

Buffers

Buffers are another essential addition to an electrophoresis experiment. Buffers ensure that the pH remains stable so that proteins or nucleic acids are not denatured. Furthermore, buffers provide the necessary ions for maintaining a constant current. Frequently used buffers include TBE (tris-borate-EDTA) and TAE (Tris-acetate-EDTA). When selecting a buffer for your experiment, it is necessary to consider various properties of the buffer, such as the molecular size, formal charges, and pKa value.

Detergents

Detergents are crucial components of the electrophoretic separation of proteins. Detergents induce unfolding of proteins and provide a negatively-charged coating which enables migration and separation. Sodium dodecyl sulfate (SDS) is a commonly-used detergent that is available in a range of concentrations.