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Electrophoresis Additives on ZAGENO

Opti-Protein XL Marker Applied Biological Materials
Reagent Type Protein ladder Form Suspension Quantity 500 µl
From $ 110.00 (500 µl)
Sizes 1 (500 µl)
Catalog IDs G266
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TrackIt™ 100 bp DNA Ladder Invitrogen
Reagent Type / Form / Quantity /
From Not available
Sizes 1 (100 Reactions)
Catalog IDs 10488058
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GeneRuler Ultra Low Range DNA Ladder Thermo Scientific
Reagent Type DNA ladder Form / Quantity /
From $ 75.75 (1 Pack)
Sizes 2 (1 Pack)
Catalog IDs SM1211, SM1212
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100 bp DNA Ladder ready to use Bioron
Reagent Type DNA ladder Form Powder Quantity 50 µg, 250 µg
From $ 39.00 (50 µg)
Sizes 2 (50 - 250 µg)
Catalog IDs 306009, 306017
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dsRNA Marker Abnova
Reagent Type RNA ladder Form / Quantity 25 µg
From $ 320.00 (25 μg)
Sizes 1 (25 μg)
Catalog IDs R0005
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ZR 1 kb DNA Marker™ Zymo Research
Reagent Type DNA ladder Form Powder Quantity 50 µg, 200 µg
From $ 56.00 (50 µg)
Sizes 3 (50 - 200 µg)
Catalog IDs M5003-50, M5006-50, ...
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DNAmark 1kb PLUS Marker G-Biosciences
Reagent Type DNA ladder Form Powder Quantity 50 µg
From $ 60.00 (50 µg)
Sizes 1 (50 µg)
Catalog IDs 786-854
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Ethidium bromide solution Sigma-Aldrich
Reagent Type Stains & dyes Form Suspension Quantity 10 ml
From $ 59.40 (10 ml)
Sizes 1 (10 ml)
Catalog IDs E1510-10ML
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50 bp plus DNA Ladder no stain Bioron
Reagent Type DNA ladder Form Powder Quantity 50 µg, 250 µg
From $ 39.00 (50 µg)
Sizes 2 (50 - 250 µg)
Catalog IDs 304008, 304016
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HyperLadder 1kb Bioline
Reagent Type / Form Suspension Quantity 500 µl, 1000 µl, 2500 µl
From $ 62.50 (500 µl)
Sizes 3 (200 - 500 Lanes)
Catalog IDs BIO-33025, BIO-33026, ...
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HyperLadder 25bp Bioline
Reagent Type / Form Suspension Quantity 500 µl, 1000 µl, 2500 µl
From $ 62.50 (100 Lanes)
Sizes 3 (100 - 500 Lanes)
Catalog IDs BIO-33057, BIO-33031, ...
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Electrophoresis

Electrophoresis is the process by which DNA, RNA, and proteins are separated according to their size. Negatively charged nucleic acids or proteins migrate towards a positive electrode at a speed relative to their size, with smaller fragments traveling faster, and ultimately further. Various electrophoresis techniques are used in biological research. These include horizontal and vertical gel electrophoresis, agarose gel electrophoresis, polyacrylamide gel electrophoresis, and SDS-PAGE. Numerous additives are required to carry out electrophoresis successfully.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Standards

Molecular standards are essential in determining the approximate size of the macromolecules being analyzed. There are a wide range of DNA, RNA and protein ladders available in a range of sizes that can be used on either polyacrylamide or agarose gels.

Loading Dyes

Loading dyes and stains are both fundamental components of electrophoresis. Loading dyes are added to the nucleic acid or protein sample to increase the density of the sample and prevent it from diffusing into the buffer. Loading stains visualize the bands following electrophoresis, enabling analysis. The most commonly used loading stain for visualizing DNA/RNA is ethidium bromide, which fluoresces under UV light. Coomassie Brilliant Blue Dye is routinely used to visualize proteins.

Buffers

Buffers are another essential addition to an electrophoresis experiment. Buffers ensure that the pH remains stable so that proteins or nucleic acids are not denatured. Furthermore, buffers provide the necessary ions for maintaining a constant current. Frequently used buffers include TBE (tris-borate-EDTA) and TAE (Tris-acetate-EDTA). When selecting a buffer for your experiment, it is necessary to consider various properties of the buffer, such as the molecular size, formal charges, and pKa value.

Detergents

Detergents are crucial components of the electrophoretic separation of proteins. Detergents induce unfolding of proteins and provide a negatively-charged coating which enables migration and separation. Sodium dodecyl sulfate (SDS) is a commonly-used detergent that is available in a range of concentrations.