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Crystal Taq Master Lyophilisate SKU: PCR-152
Hot-Start PCR
/
Multiplex PCR
/
Templates
/
Scientific Score
1.33
Brand
Jena Bioscience
Sizes
2 (192 - 960 Reactions)
Available From
1 Vendor
Price Range
$ 144.00 - $ 578.00
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TaqProbe qPCR MasterMix - No Dye
Multiplex PCR
/
Reference Dye
/
Templates
Genomic DNA, Plasmid DNA, cDNA
Genomic DNA, Plasmid DNA, cDNA
Scientific Score
9.88
Brand
Applied Biological Materials
Sizes
1 (500 Reactions)
Available From
1 Vendor
Price
$ 125.00
Catalog IDs
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illustra PuReTaq Ready-To-Go PCR Beads (Multiwell Plate)
Hot-Start PCR
/
Multiplex PCR
/
Templates
Genomic DNA, Plasmid DNA
Genomic DNA, Plasmid DNA
Scientific Score
6.32
Brand
GE Healthcare Life Sciences
Sizes
2 (96 - 480 Reactions)
Available From
1 Vendor
Price
$ 183.10
Catalog IDs
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Expand™ High FidelityPLUS PCR System, dNTPack
Hot-Start PCR
/
Multiplex PCR
/
Templates
Genomic DNA
Genomic DNA
Scientific Score
6.46
Brand
Roche Life Science
Sizes
2 (125 - 500 Reactions)
Available From
1 Vendor
Price Range
$ 144.00 - $ 528.00
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Extract-N-Amp™ Blood PCR Kit
Hot-Start PCR
/
Multiplex PCR
/
Templates
Genomic DNA
Genomic DNA
Scientific Score
8.67
Brand
Sigma-Aldrich
Sizes
2 (100 - 1000 Reactions)
Available From
2 Vendors
Price Range
$ 225.00 - $ 1,610.27
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Betaine SKU : 61962
Reagent Type
/
Product Application
/
Concentration
/
Scientific Score
9.96
Brand
Sigma-Aldrich
Sizes
1 (250 g)
Available From
2 Vendors
Price Range
$ 182.00 - $ 227.10
Catalog IDs
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Inhibitor Resistant Genotyping PCR ReadyMix™, ROX™
Multiplex PCR
/
Reference Dye
ROX
ROX
Templates
Genomic DNA, Plasmid DNA, cDNA
Genomic DNA, Plasmid DNA, cDNA
Scientific Score
9.95
Brand
Sigma-Aldrich
Sizes
3 (250 - 5000 Reactions)
Available From
2 Vendors
Price Range
$ 159.59 - $ 180.00
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KiCqStart® Probe qPCR ReadyMix™, ROX™
Multiplex PCR
/
Reference Dye
ROX
ROX
Templates
Genomic DNA, cDNA
Genomic DNA, cDNA
Scientific Score
9.97
Brand
Sigma-Aldrich
Sizes
3 (250 - 5000 Reactions)
Available From
2 Vendors
Price Range
$ 159.59 - $ 180.00
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FastStart TaqMan® Probe Master
Multiplex PCR
/
Reference Dye
/
Templates
Genomic DNA, Plasmid DNA, cDNA
Genomic DNA, Plasmid DNA, cDNA
Scientific Score
9.96
Brand
Roche Life Science
Sizes
3 (100 - 2000 Reactions)
Available From
1 Vendor
Price Range
$ 195.00 - $ 913.00
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SYBR® Green JumpStart™ Taq ReadyMix™
Multiplex PCR
/
Reference Dye
/
Templates
Genomic DNA, Plasmid DNA
Genomic DNA, Plasmid DNA
Scientific Score
10.00
Brand
Sigma-Aldrich
Sizes
3 (20 - 500 Reactions)
Available From
2 Vendors
Price Range
$ 45.80 - $ 951.00
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illustra Hot Start Mix RTG
Hot-Start PCR
/
Multiplex PCR
/
Templates
Genomic DNA, Plasmid DNA
Genomic DNA, Plasmid DNA
Scientific Score
7.19
Brand
GE Healthcare Life Sciences
Sizes
3 (96 - 480 Reactions)
Available From
2 Vendors
Price Range
$ 183.25 - $ 898.00
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Expand™ Long Template PCR System
Hot-Start PCR
/
Multiplex PCR
/
Templates
Genomic DNA, cDNA
Genomic DNA, cDNA
Scientific Score
9.68
Brand
Roche Life Science
Sizes
3 (38 - 950 Reactions)
Available From
1 Vendor
Price Range
$ 135.00 - $ 2,640.00
Added to comparison remove item

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PCR Amplification

The polymerase chain reaction (PCR) is an in vitro method used for the amplification of a specific DNA template to generate a large number of copies in the span of just a few hours. The development of the PCR method in 1983 by Kary Mullis replaced the old and slow cloning techniques which required days of work.

PCR Kits and Methods

Real-Time PCR

Real-time PCR is a technique that monitors DNA amplification in real-time rather than at the end of the PCR run, as is the case for End-Point PCR.

End-Point PCR

PCR enables amplification of specific DNA fragments with the help of a thermocycler and is a widespread molecular biology technique.

PCR Reagents

PCR requires five reagents; DNA/RNA template, DNA polymerase, primers (forward and reverse), dNTPs and PCR buffers.

The benefit of PCR

Another benefit of the PCR method is its high sensitivity; theoretically, a single copy of the template is enough to start the reaction. The sensitivity and speed make the PCR techniques useful in both basic research and for commercial applications like in vitro diagnostics, forensics, and quality control.

The basic principles of the PCR method

To start a PCR reaction the following is needed:

  • A template: a DNA or RNA strand that we want to amplify.

  • Nucleotides (dCTP, dGTP, dATP, dGTP): the building blocks from which DNA is made.Primers: short nucleotide strands that will anneal to the position that we want to start and end the amplification.

  • DNA polymerase: the enzyme catalyzing the reaction.

  • (Accessory additives like buffers and Mg2+)

Our cDNA synthesis Troubleshoot will guide you though any initial setbacks.

The amplification process:

  • Denaturation: the DNA molecule is split into two single strands of DNA by heating (carried out in a PCR thermocycler)

  • Hybridization: During a cool-down phase, the primers anneal to the target sequences of the single stranded DNA

  • Elongation: Polymerase extends the DNA molecule starting at the primer sequences, and so doubles the template numbers

  • The process is repeated from Step 1 achieving an exponential increase in the number of templates until the substrate runs out or the product accumulation is halting the reaction.

Check out our End-Point PCR Troubleshoot if you encounter any problems.

The past, present and future of PCR

PCR was originally developed to detect specific DNA sequences in a sample, and though this is still the most used application, many other applications have been developed over the past decades.
Nowadays there exist different types of PCRs e.g. End-Point PCR, qPCR (quantitative Real-time PCR), RT-PCR (Reverse Transcription PCR), Multiplex PCR, Nested PCR and Digital PCR. Depending on the polymerases used, the PCR is featured with special properties like a hot start mechanism or proofreading activity to enable the best results.

See our Real-Time PCR Troubleshoot as well as the other troubleshooting guides for tips to perfect your experiment.

Video and Image Credits:

Video: DNA Learning Center/YouTube