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End-Point PCR Kits

ACTaq™ High Fidelity Mastermix 2x

ACTGene

From
$ 50.95 (50 Reactions)
Sizes
2 (50 - 200 Reactions)
Catalog IDs
E2010-50RXN, E2010-200RXN
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PCR

Heat-Resistant Films for PCR, Polypropylene, Nonsterile

VWR International

From
$ 88.31 (100 Films)
Sizes
1 (100 Films)
Catalog IDs
60941-070
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Real-Time PCR Kits

AccuPower Plus DualStar™ qPCR 2X Master Mix

Bioneer

Multiplex PCR
Multiplex PCR
Multiplex PCR
Reference Dye
ROX
ROX
Templates
Genomic DNA, Plasmid DNA, Viral DNA, cDNA, dsDNA
Genomic DNA, Plasmid DNA, Viral DNA, cDNA, dsDNA
From
$ 127.62 (250 Reactions)
Sizes
4 (250 - 5000 Reactions)
Catalog IDs
K-6603, K-6603-1, ...
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PCR Reagents

Green Taq DNA Polymerase

GenScript

Reagent Type
/
/
Product Application
/
/
Concentration
/
/
From
$ 60.00 (1000 U)
Sizes
1 (1000 U)
Catalog IDs
E00043
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PCR Reagents

PrimeSTAR® GXL Premix

TaKaRa

From
$ 281.00 (200 Reactions)
Sizes
2 (200 - 800 Reactions)
Catalog IDs
R051A, R051B
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Real-Time PCR Kits

Fast EvaGreen Master Mix for qPCR(200 rxn)

Biotium

Multiplex PCR
/
/
Reference Dye
/
/
Templates
/
/
From
$ 63.00 (1 ml)
Sizes
3 (1 - 5 ml)
Catalog IDs
31003-T, 31003, ...
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PCR Reagents

Individual Reaction Mix 1

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-1
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PCR Reagents

Individual Reaction Mix 6

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-6
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PCR Reagents

Individual Reaction Mix 8

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-8
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PCR Reagents

Individual Reaction Mix 9

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-9
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End-Point PCR Kits

One-Step RT-PCR Kit with dye

Applied Biological Materials

From
$ 195.00 (5000 μl)
Sizes
1 (5000 μl)
Catalog IDs
G174-dye
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PCR Reagents

RT-PCR Control Template Kit

Applied Biological Materials

From
$ 165.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
G928
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PCR Amplification

The polymerase chain reaction (PCR) is an in vitro method used for the amplification of a specific DNA template to generate a large number of copies in the span of just a few hours. The development of the PCR method in 1983 by Kary Mullis replaced the old and slow cloning techniques which required days of work.

PCR Kits and Methods

Real-Time PCR

Real-time PCR is a technique that monitors DNA amplification in real-time rather than at the end of the PCR run, as is the case for End-Point PCR.

End-Point PCR

PCR enables amplification of specific DNA fragments with the help of a thermocycler and is a widespread molecular biology technique.

PCR Reagents

PCR requires five reagents; DNA/RNA template, DNA polymerase, primers (forward and reverse), dNTPs and PCR buffers.

The benefit of PCR

Another benefit of the PCR method is its high sensitivity; theoretically, a single copy of the template is enough to start the reaction. The sensitivity and speed make the PCR techniques useful in both basic research and for commercial applications like in vitro diagnostics, forensics, and quality control.

The basic principles of the PCR method

To start a PCR reaction the following is needed:

  • A template: a DNA or RNA strand that we want to amplify.

  • Nucleotides (dCTP, dGTP, dATP, dGTP): the building blocks from which DNA is made.Primers: short nucleotide strands that will anneal to the position that we want to start and end the amplification.

  • DNA polymerase: the enzyme catalyzing the reaction.

  • (Accessory additives like buffers and Mg2+)

Our cDNA synthesis Troubleshoot will guide you though any initial setbacks.

The amplification process:

  • Denaturation: the DNA molecule is split into two single strands of DNA by heating (carried out in a PCR thermocycler)

  • Hybridization: During a cool-down phase, the primers anneal to the target sequences of the single stranded DNA

  • Elongation: Polymerase extends the DNA molecule starting at the primer sequences, and so doubles the template numbers

  • The process is repeated from Step 1 achieving an exponential increase in the number of templates until the substrate runs out or the product accumulation is halting the reaction.

Check out our End-Point PCR Troubleshoot if you encounter any problems.

The past, present and future of PCR

PCR was originally developed to detect specific DNA sequences in a sample, and though this is still the most used application, many other applications have been developed over the past decades.
Nowadays there exist different types of PCRs e.g. End-Point PCR, qPCR (quantitative Real-time PCR), RT-PCR (Reverse Transcription PCR), Multiplex PCR, Nested PCR and Digital PCR. Depending on the polymerases used, the PCR is featured with special properties like a hot start mechanism or proofreading activity to enable the best results.

See our Real-Time PCR Troubleshoot as well as the other troubleshooting guides for tips to perfect your experiment.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video and Image Credits:

Video: DNA Learning Center/YouTube