Experience more EUREKA moments
CUSTOMER SERVICE +1 617 855 5944 +1 617 855 5944

End-Point PCR Kits

Horse-Power™ Red-Taq DNA Polymerase, MasterMix (2.5x)

Canvax Biotech

From
$ 48.00 (100 Reactions)
Sizes
2 (100 - 500 Reactions)
Catalog IDs
P0027-S, P0027
Added to comparison remove item

End-Point PCR Kits

Hot Start PCR-TO-GEL TAQ PCR Master Mix, 2X

AMRESCO

From
$ 418.29 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
1B1409-500RXN
Added to comparison remove item

End-Point PCR Kits

ACTaq™ High Fidelity Mastermix 2x

ACTGene

From
$ 50.95 (50 Reactions)
Sizes
2 (50 - 200 Reactions)
Catalog IDs
E2010-50RXN, E2010-200RXN
Added to comparison remove item

End-Point PCR Kits

Maxime™ RT PreMix Kit (Oligo dT Primer)

iNtRON

Hot-Start PCR
/
/
Multiplex PCR
/
/
Templates
/
/
From
$ 99.00 (96 Tubes)
Sizes
1 (96 Tubes)
Catalog IDs
25081
Added to comparison remove item

End-Point PCR Kits

One-Step RT-PCR Kit with dye

Applied Biological Materials

From
$ 195.00 (5000 μl)
Sizes
1 (5000 μl)
Catalog IDs
G174-dye
Added to comparison remove item

End-Point PCR Kits

2X SensTaq HotStart MasterMix

Applied Biological Materials

From
$ 115.00 (5 ml)
Sizes
1 (5 ml)
Catalog IDs
G939
Added to comparison remove item

End-Point PCR Kits

In Vitro Director™ PCR System SKU : ID0010

Sigma-Aldrich

From
$ 394.50 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
ID0010-1KT
Added to comparison remove item

End-Point PCR Kits

TAQ CORE KIT 25

MP Biomedicals

Hot-Start PCR
/
/
Multiplex PCR
/
/
Templates
/
/
From
$ 197.10 (250 Units)
Sizes
4 (3 - 2500 Units)
Catalog IDs
11EPTQK253, 11EPTQK251, ...
Added to comparison remove item

End-Point PCR Kits

TransDirect® Plant Tissue PCR Kit

TransGen Biotech

Hot-Start PCR
/
/
Multiplex PCR
/
/
Templates
/
/
From
$ 123.08 (100 Reactions)
Sizes
2 (100 - 500 Reactions)
Catalog IDs
AD301-01, AD301-02
Added to comparison remove item

End-Point PCR Kits

2×EasyTaq® PCR SuperMix ( +dye )

TransGen Biotech

Hot-Start PCR
/
/
Multiplex PCR
/
/
Templates
/
/
From
$ 27.69 (1 ml)
Sizes
4 (1 - 480 ml)
Catalog IDs
AS111-11, AS111-12, ...
Added to comparison remove item

End-Point PCR Kits

SensiFAST™ Probe No-ROX Kit, from Bioline

Bioline

From
$ 2,500.00 (5000 Reactions)
Sizes
1 (5000 Reactions)
Catalog IDs
BIO-86050
Added to comparison remove item

End-Point PCR Kits

SensiFAST™ SYBR® Lo-ROX Kit, from Bioline

Bioline

From
$ 2,500.00 (5000 Reactions)
Sizes
1 (5000 Reactions)
Catalog IDs
BIO-94050
Added to comparison remove item

Can't find what you are looking for? Request the product now and we'll find it for you (typically within 1 hour).

Request Product

Conventional PCR Amplification

Conventional (end-point) polymerase chain reaction (PCR) enables amplification of specific DNA fragments with the help of a thermocycler and is one of the most widespread molecular biology techniques, with endless applications spanning molecular cloning to genotyping, to semi-quantitative gene expression analysis to molecular diagnostics.

End-Point PCR

End-Point PCR is distinguishable from real-time PCR as it is not quantitative; the results are instead visualized on a gel once the run has finished.

There are of course a variety of kits available on the market that are providing all the necessary PCR reagents for a successful End-Point PCR reaction. When choosing a kit, there are a few things to consider when picking the one for your experiments:

The first is templates, as DNA and RNA require different procedures. For example, if you are performing Reverse-Transcription PCR (RT-PCR), an RNA template is required to produce complementary DNA (cDNA). Such RT-PCR procedures are often divided into one- or two-step PCRs.

Next, choosing the right polymerase can be crucial for a successful experimental outcome.

Polymerases may differ in their processivity. The processivity is defined as the number of bases they will read on a DNA strand before falling off the template. This is important for cloning of long fragments to avoid truncated amplicons.

Some polymerases can also have proofreading activity, like the famous Klenow polymerase, which ensures that amplified fragments are a high fidelity copy of the original. This is generally measured as average mistakes per bases amplified and should be kept as low as possible to avoid unexpected mutations particularly during cloning experiments. If high fidelity is not essential (like for confirming plasmid inserts), then Taq polymerase is a good, cost-effective alternative.

Hot start PCR compatible polymerases can also help you to get rid of unwanted non-specific amplification.

The polymerase processing speed is another factor to consider. High-speed polymerases can save you time when using PCR programs with a long primer extension cycles.

Complex applications such as multiplex PCR, where the amplification of multiple targets in the same reaction occurs, or the creation of labeled DNA probes using modified dNTPs, also have dedicated kits to make your experimentation easier.

Our End-Point PCR Troubleshoot will simplify the process further.

Compare Reverse Transcription End-Point PCR Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video Credits

Video: Oxford Academic (Oxford University Press)/YouTube