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End-Point PCR Kits on ZAGENO

AccuPower RT-PCR PreMix, 1 x 96-well flat plate, 20 µl rxn Bioneer
Templates mRNA, Total RNA, Viral RNA Fidelity / Amplicon Size /
From $ 251.80 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-2262-4
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AccuPower RocketScript™ RT-PCR PreMix, 0.2 ml 8-tube strip w/ cap, 50 µl x 96 rxns Bioneer
Templates mRNA, Total RNA, Viral RNA Fidelity / Amplicon Size /
From $ 734.20 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-2503
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Tissue Ex-Amp PCR Kit (with Kodaq MasterMix) Applied Biological Materials
Templates Genomic DNA Fidelity Proofreading activity Amplicon Size /
From $ 85.00 (120 Reactions)
Sizes 1 (120 Reactions)
Catalog IDs G927
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REDTaq® DNA Polymerase Sigma-Aldrich
Templates Genomic DNA Fidelity No proofreading activity Amplicon Size /
From $ 46.30 (50 Units)
Sizes 3 (50 - 1000 Units)
Catalog IDs D4309-50UN, D4309-250UN, ...
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2X One-Step RT-PCR Master Mix Norgen Biotek
Templates Total RNA Fidelity No proofreading activity Amplicon Size < 1.5 kb
From $ 243.00 (100 Reactions)
Sizes 3 (100 - 500 Reactions)
Catalog IDs 28113, 28114, ...
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PCR Master Roche Life Science
Templates Genomic DNA, cDNA Fidelity No proofreading activity Amplicon Size < 2 kb
From $ 261.00 (200 Reactions)
Sizes 1 (200 Reactions)
Catalog IDs 11636103001
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MyTaq™ Blood-PCR Kit Bioline
Templates Genomic DNA Fidelity No proofreading activity Amplicon Size /
From $ 221.00 (250 Reactions)
Sizes 1 (250 Reactions)
Catalog IDs BIO-25054
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SD Polymerase Hotstart, 10 U/ul Bioron
Templates cDNA, Genomic DNA Fidelity No proofreading activity Amplicon Size > 20 – 30 kb
From $ 120.00 (200 Units)
Sizes 2 (200 - 1000 Units)
Catalog IDs 108902, 108910
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Phusion™ Flash High-Fidelity PCR Master Mix Thermo Scientific
Templates / Fidelity No proofreading activity Amplicon Size /
From $ 84.75 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs F548S
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REDExtract-N-Amp™ Blood PCR Kit Sigma-Aldrich
Templates Genomic DNA Fidelity No proofreading activity Amplicon Size /
From $ 32.60 (10 Reactions)
Sizes 4 (10 - 1000 Reactions)
Catalog IDs XNABS-1KT, XNAB-1KT, ...
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Pwo Master Roche Life Science
Templates Plasmid DNA, Genomic DNA Fidelity Proofreading activity Amplicon Size < 3 kb
From $ 215.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs 3789403001
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Tth Polymerase Bioron
Templates Total RNA Fidelity No proofreading activity Amplicon Size > 2-3 kb
From $ 126.00 (500 Units)
Sizes 2 (500 - 2500 Units)
Catalog IDs 104005, 104025
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Conventional PCR Amplification

Conventional (end-point) polymerase chain reaction (PCR) enables amplification of specific DNA fragments with the help of a thermocycler and is one of the most widespread molecular biology techniques, with endless applications spanning molecular cloning to genotyping, to semi-quantitative gene expression analysis to molecular diagnostics.

End-Point PCR

End-Point PCR is distinguishable from real-time PCR as it is not quantitative; the results are instead visualized on a gel once the run has finished.

There are of course a variety of kits available on the market that are providing all the necessary PCR reagents for a successful End-Point PCR reaction. When choosing a kit, there are a few things to consider when picking the one for your experiments:

  • The first is templates, as DNA and RNA require different procedures. For example, if you are performing Reverse-Transcription PCR (RT-PCR), an RNA template is required to produce complementary DNA (cDNA). Such RT-PCR procedures are often divided into one- or two-step PCRs.
  • Next, choosing the right polymerase can be crucial for a successful experimental outcome.

Polymerases may differ in their processivity. The processivity is defined as the number of bases they will read on a DNA strand before falling off the template. This is important for cloning of long fragments to avoid truncated amplicons.

Some polymerases can also have proofreading activity, like the famous Klenow polymerase, which ensures that amplified fragments are a high fidelity copy of the original. This is generally measured as average mistakes per bases amplified and should be kept as low as possible to avoid unexpected mutations particularly during cloning experiments. If high fidelity is not essential (like for confirming plasmid inserts), then Taq polymerase is a good, cost-effective alternative.

Hot start PCR compatible polymerases can also help you to get rid of unwanted non-specific amplification.

The polymerase processing speed is another factor to consider. High-speed polymerases can save you time when using PCR programs with a long primer extension cycles.

Complex applications such as multiplex PCR, where the amplification of multiple targets in the same reaction occurs, or the creation of labeled DNA probes using modified dNTPs, also have dedicated kits to make your experimentation easier.

Our End-Point PCR Troubleshoot will simplify the process further.

Compare Reverse Transcription End-Point PCR Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.

Video Credits

Video: Oxford Academic (Oxford University Press)/YouTube