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PCR Reagents

Score 9.76

PrimeSTAR® GXL Premix

TaKaRa

From
$ 281.00 (200 Reactions)
Sizes
2 (200 - 800 Reactions)
Catalog IDs
R051A, R051B
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PCR Reagents

Score 9.24

Individual Reaction Mix 1

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-1
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PCR Reagents

Score 9.24

Individual Reaction Mix 6

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-6
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PCR Reagents

Score 9.23

Individual Reaction Mix 8

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-8
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PCR Reagents

Score 9.24

Individual Reaction Mix 9

Applied Biological Materials

From
$ 125.00 (200 Reactions)
Sizes
1 (200 Reactions)
Catalog IDs
G065-9
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PCR Reagents

Score 9.24

RT-PCR Control Template Kit

Applied Biological Materials

From
$ 165.00 (100 Reactions)
Sizes
1 (100 Reactions)
Catalog IDs
G928
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PCR Reagents

Score 9.53

Accuris™ 1 Hour Mammalian Genotyping Kit SKU: PR1300-MG

Benchmark Scientific

Reagent Type
/
/
Product Application
/
/
Concentration
/
/
From
$ 6.44 (8 Reactions)
Sizes
3 (8 - 400 Reactions)
Catalog IDs
PR1300-MG-S, PR1300-MG-80, ...
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PCR Reagents

Score 9.53

Accuris™ qMax cDNA Synthesis Kit SKU: PR2100-C

Benchmark Scientific

Reagent Type
/
/
Product Application
/
/
Concentration
/
/
From
$ 13.80 (5 Reactions)
Sizes
4 (5 - 250 Reactions)
Catalog IDs
PR2100-C-S, PR2100-C-25, ...
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PCR Reagents

Score 9.51

Accuris™ qMax Probe qPCR Mix SKU: PR2001-H

Benchmark Scientific

Reagent Type
/
/
Product Application
/
/
Concentration
/
/
From
$ 9.20 (20 Reactions)
Sizes
4 (20 - 1000 Reactions)
Catalog IDs
PR2001-H-S, PR2001-H-100, ...
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PCR Reagents

Score 9.51

Accuris™ qMax Probe qPCR Mix SKU: PR2001-L

Benchmark Scientific

Reagent Type
/
/
Product Application
/
/
Concentration
/
/
From
$ 9.20 (20 Reactions)
Sizes
4 (20 - 1000 Reactions)
Catalog IDs
PR2001-L-S, PR2001-L-100, ...
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PCR Reagents

Score 9.86

SafeView™ Classic

Applied Biological Materials

Reagent Type
Staining solutions
Staining solutions
Product Application
Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
Safe Detection of dsDNA, ssDNA and RNA in agarose and polyacrylamide gels.
Concentration
/
/
From
$ 30.00 (1000 µl)
Sizes
1 (1000 µl)
Catalog IDs
G108
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PCR Reagents

Score 9.80

ROX Reference Dye

Jena Bioscience

Reagent Type
Reference dyes
Reference dyes
Product Application
Fluorescence signal normalization
Fluorescence signal normalization
Concentration
50X
50X
From
$ 39.00 (500 Reactions)
Sizes
1 (500 Reactions)
Catalog IDs
PCR-351
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Polymerase chain reaction (PCR) is a useful molecular biology technique developed by Kary Mullis in the 1980s. PCR is used to amplify DNA templates into millions of copies of a particular DNA sequence within hours. Due to its powerful ability, PCR is applied in different areas such as; genetic and infectious disease identification, forensics, and research in molecular biology. In contrast to conventional PCR, quantitative reverse transcriptase PCR (qRT-PCR) allows measurement of gene expression quantitatively and in real-time.

In general, a complete PCR reaction requires five basic PCR reagents; DNA/RNA template, DNA polymerase, primers (forward and reverse), deoxynucleotide triphosphates (dNTPs) and PCR buffers.

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PCR templates

Selecting the right templates for PCR is vital as different templates are used for the various methods of PCR. For instance, when performing RT-qPCR, RNA templates are required for producing complementary DNA while DNA templates are needed for conventional PCR. It is also important to have DNA or RNA templates in high quality and quantity as this can help optimizing the efficiency of PCR. For this reason, a good PCR template preparation kit is needed for the first step of a successful PCR.

DNA polymerase

All PCR reactions require a DNA polymerase which can work under high temperature (around 70 °C) as the first phase of PCR involves separation of DNA strands at high temperature (~90 °C). Taq polymerase, which is a heat-stable enzyme isolated from the thermophilic bacterium Thermus aquaticus is a commonly used DNA polymerase for PCR. Some DNA polymerases are engineered to be activated at high temperature only, so as to reduce non-specific amplification at the beginning stage of PCR.

Primers

The initiation of DNA synthesis requires primers; short strands of nucleotides (DNA or RNA) which are complementary to the template DNA and serve as a DNA synthesis starting point for the DNA/RNA polymerase. Annealing of primers to single strand DNA requires lower temperature (50-65 °C) than the denaturation step. Once the annealing step is completed, hydrogen bonds will form between the primers and the template DNA.

Deoxynucleotide triphosphates (dNTPs)

Deoxynucleotide triphosphates (dNTPs) are the essential components of PCR as they act as the building blocks of nucleic acids; DNA polymerase cannot synthesize DNA without a supply of dNTPs.

PCR buffers

PCR buffers ensure that the PCR reaction is conducted under optimal conditions. The major components of PCR buffer include Tris-HCl, potassium chloride (KCl) and magnesium chloride (MgCl2). Tris-HCl and KCl are responsible for maintaining a stable pH during PCR. Magnesium ions act as cofactors for DNA polymerase so as to ensure proper DNA synthesis function of the polymerase during PCR. PCR buffer is usually available at 10X concentration.

Compare PCR Reagents!

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