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Real-time PCR (qPCR) Kits on ZAGENO

ExCellenCT One-Step EvaGreen qRT-PCR Kit - iCycler Applied Biological Materials
Fluorescent Dye Included Templates / Amplicon Size < 150 bp
From $ 135.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs G917-iC
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ProbeMasterMix (2X) High ROX for qPCR Genaxxon Bioscience
Fluorescent Dye Not included Templates Genomic DNA, cDNA, Plasmid DNA Amplicon Size /
From $ 135.70 (2.5 ml)
Sizes 2 (2.5 - 12.5 ml)
Catalog IDs M3010.0100, M3010.0500
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GreenHot Master Mix Bioron
Fluorescent Dye Included Templates cDNA Amplicon Size /
From $ 96.00 (200 Reactions)
Sizes 2 (200 - 1000 Reactions)
Catalog IDs 119205, 119225
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SYBR GreenER™ qPCR SuperMix Universal Invitrogen
Fluorescent Dye Included Templates cDNA, Genomic DNA, Plasmid DNA Amplicon Size 80-250 bp
From $ 231.00 (100 Reactions)
Sizes 3 (100 - 2000 Reactions)
Catalog IDs 11762-100, 11762-500, ...
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TaqMan® PreAmp Master Mix Applied Biosystems
Fluorescent Dye Not included Templates cDNA Amplicon Size /
From $ 932.00 (40 Reactions)
Sizes 2 (40 - 200 Reactions)
Catalog IDs 4391128, 4488593
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AMPIGENE® qPCR 1-Step Green Kit Hi-ROX Enzo Life Sciences
Fluorescent Dye Not included Templates Total RNA, mRNA Amplicon Size 80-200 bp
From $ 250.00 (200 Reactions)
Sizes 2 (200 - 1000 Reactions)
Catalog IDs ENZ-NUC109-0200, ENZ-NUC109-1000
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Luminaris Color HiGreen qPCR Master Mix Thermo Scientific
Fluorescent Dye Included Templates Plasmid DNA, Genomic DNA, cDNA, Viral DNA Amplicon Size 70-150 bp
From $ 183.00 (250 Reactions)
Sizes 4 (250 - 5000 Reactions)
Catalog IDs K0391, K0392, ...
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Fast SYBR® Green Master Mix Applied Biosystems
Fluorescent Dye Included Templates Genomic DNA, cDNA Amplicon Size 50-150 bp
From $ 100.00 (100 Reactions)
Sizes 6 (100 - 5000 Reactions)
Catalog IDs 4385610, 4385612, ...
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iQ™ SYBR® Green Supermix Bio-Rad
Fluorescent Dye Included Templates Genomic DNA, cDNA Amplicon Size 70-150 bp
From $ 225.00 (100 Reactions)
Sizes 7 (100 - 2000 Reactions)
Catalog IDs 1708880, 1708882, ...
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TaqMan® Fast Virus 1-Step Master Mix Applied Biosystems
Fluorescent Dye Not included Templates Viral DNA, Total RNA, mRNA, Viral RNA Amplicon Size /
From $ 344.00 (200 Reactions)
Sizes 3 (200 - 2000 Reactions)
Catalog IDs 4444432, 4444434, ...
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TaqMan® Universal PCR Master Mix Applied Biosystems
Fluorescent Dye Not included Templates cDNA, Genomic DNA, Plasmid DNA Amplicon Size 50-150 bp
From $ 522.00 (500 Reactions)
Sizes 6 (500 - 5000 Reactions)
Catalog IDs 4304437, 4364338, ...
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qPCR Function

Real-time PCR is a technique that monitors DNA amplification in real-time rather than at the end of the PCR run, as is the case for End-Point PCR. With the added step of fluorescent labeling, it is possible to quantify the abundance of the amplified DNA using so-called quantitative real-time PCR (qPCR). When qPCR is used in combination with reverse transcription and cDNA synthesis, this is called RT-qPCR, a common technique for the analysis of gene expression.

Read about how qPCR has aided recent research: HIV 'Cure'? Kick and Kill Breakthrough

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

How does Real-Time PCR work?

Here at ZAGENO, you can find kits for different templates, such as:

Real-time PCR (qPCR) kits will provide polymerases, oligonucleotides, and buffers for your experiments. Before comparing kits from different suppliers on our product page, though, there are a few factors to consider:

  • You should keep in mind the size of your amplicon. While in general qPCR amplifies short fragments of around 150 bases, there are polymerases with higher processivity. A side note: if you plan on performing Hot Start qPCR (prevents non-specific amplification at room temperature), look for kits including enzymes suitable for this application.

  • If you expect to amplify a template present in low quantities in your sample, consider kits that use SYTO 9 as an intercalating dye, which has higher sensitivity. For standard applications, SYBR Green I is still an excellent choice.

  • Alternatively, probe-based qPCR, use labeled fluorescent probes instead of intercalates. In doing so provides excellent sensitivity for sequence–specific detection, but need to be prepared specifically for your template beforehand.

  • If you are using RT-qPCR, make sure to pick a one- or two-step kit, according to your experimental setup.

ZAGENO's Real-Time PCR Troubleshoot will help guide you through any diffculties encountered.

Compare Real-Time PCR Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the TAKYON™ ONE-STEP ROX PROBE 5X MASTERMIX DTTP kit from Eurogentec is the cheapest per reaction.

  • that the ITAQ™ UNIVERSAL SYBR® GREEN ONE-STEP KIT from Bio-Rad includes a fluorescent dye.

  • that the TAQMAN® FAST VIRUS 1-STEP MASTER MIX from Applied Biosystems can also use Viral DNA as a template.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video Credits

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