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FastStart Universal Probe Master (Rox)
Multiplex PCR
/
Reference Dye
ROX
ROX
Templates
Genomic DNA, Plasmid DNA, cDNA
Genomic DNA, Plasmid DNA, cDNA
Scientific Score
9.96
Brand
Roche Life Science
Sizes
3 (100 - 2000 Reactions)
Available From
1 Vendor
Price Range
$ 161.00 - $ 751.00
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KiCqStart® One-Step Probe RT-qPCR ReadyMix™, Low ROX™
Multiplex PCR
/
Reference Dye
Low ROX
Low ROX
Templates
Total RNA, Viral RNA, mRNA
Total RNA, Viral RNA, mRNA
Scientific Score
9.97
Brand
Sigma-Aldrich
Sizes
1 (500 Reactions)
Available From
2 Vendors
Price Range
$ 573.76 - $ 650.00
Catalog IDs
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KiCqStart® SYBR® Green qPCR ReadyMix™
Multiplex PCR
/
Reference Dye
/
Templates
Genomic DNA, cDNA
Genomic DNA, cDNA
Scientific Score
9.96
Brand
Sigma-Aldrich
Sizes
3 (250 - 5000 Reactions)
Available From
2 Vendors
Price Range
$ 265.00 - $ 273.67
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Inhibitor Resistant Genotyping PCR ReadyMix™
Multiplex PCR
/
Reference Dye
/
Templates
Genomic DNA, cDNA
Genomic DNA, cDNA
Scientific Score
9.92
Brand
Sigma-Aldrich
Sizes
3 (250 - 5000 Reactions)
Available From
2 Vendors
Price Range
$ 159.59 - $ 180.00
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EagleTaq Universal Master Mix (ROX)
Multiplex PCR
/
Reference Dye
ROX
ROX
Templates
Genomic DNA, Plasmid DNA, cDNA
Genomic DNA, Plasmid DNA, cDNA
Scientific Score
9.96
Brand
Roche Life Science
Sizes
3 (100 - 5000 Reactions)
Available From
1 Vendor
Price Range
$ 112.00 - $ 321.00
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JumpStart™ Taq ReadyMix™ for High Throughput Quantitative PCR
Multiplex PCR
/
Reference Dye
/
Templates
Genomic DNA, Plasmid DNA
Genomic DNA, Plasmid DNA
Scientific Score
9.95
Brand
Sigma-Aldrich
Sizes
3 (20 - 2000 Reactions)
Available From
2 Vendors
Price Range
$ 42.00 - $ 761.00
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AMPLIFYME SYBR No-ROX Mi
Scientific Score
8.75
Brand
BLIRT
Sizes
2 (200 - 2000 Reactions)
Available From
1 Vendor
Price
$ 227.10
Catalog IDs
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AMPLIFYME SYBR Universal Mix
Scientific Score
8.75
Brand
BLIRT
Sizes
2 (200 - 2000 Reactions)
Available From
1 Vendor
Price
$ 240.62
Catalog IDs
Added to comparison remove item
AMPLIFYME Probe No-ROX Mix
Scientific Score
8.75
Brand
BLIRT
Sizes
2 (200 - 2000 Reactions)
Available From
1 Vendor
Price
$ 227.10
Catalog IDs
Added to comparison remove item
AMPLIFYME Probe Universal Mix
Scientific Score
8.75
Brand
BLIRT
Sizes
2 (200 - 2000 Reactions)
Available From
1 Vendor
Price
$ 240.62
Catalog IDs
Added to comparison remove item
qPCR GREEN Master Mix 2X, Without ROX
Multiplex PCR
/
Reference Dye
No ROX
No ROX
Templates
/
Scientific Score
10.00
Brand
ApexBio
Sizes
1 (5 ml)
Available From
1 Vendor
Price
$ 370.20
Catalog IDs
Added to comparison remove item
Brand
Canvax Biotech
Sizes
4 (250 - 5000 Reactions)
Available From
1 Vendor
Price Range
$ 99.00 - $ 189.00
Added to comparison remove item

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qPCR Function

Real-time PCR is a technique that monitors DNA amplification in real-time rather than at the end of the PCR run, as is the case for End-Point PCR. With the added step of fluorescent labeling, it is possible to quantify the abundance of the amplified DNA using so-called quantitative real-time PCR (qPCR). When qPCR is used in combination with reverse transcription and cDNA synthesis, this is called RT-qPCR, a common technique for the analysis of gene expression.

Read about how qPCR has aided recent research: HIV 'Cure'? Kick and Kill Breakthrough

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How does Real-Time PCR work?

Here at ZAGENO, you can find kits for different templates, such as:

Genomic DNA

cDNA

mRNA

Total RNA

Real-time PCR (qPCR) kits will provide polymerases, oligonucleotides, and buffers for your experiments. Before comparing kits from different suppliers on our product page, though, there are a few factors to consider:

  • You should keep in mind the size of your amplicon. While in general qPCR amplifies short fragments of around 150 bases, there are polymerases with higher processivity. A side note: if you plan on performing Hot Start qPCR (prevents non-specific amplification at room temperature), look for kits including enzymes suitable for this application.

  • If you expect to amplify a template present in low quantities in your sample, consider kits that use SYTO 9 as an intercalating dye, which has higher sensitivity. For standard applications, SYBR Green I is still an excellent choice.

  • Alternatively, probe-based qPCR, use labeled fluorescent probes instead of intercalates. In doing so provides excellent sensitivity for sequence–specific detection, but need to be prepared specifically for your template beforehand.

  • If you are using RT-qPCR, make sure to pick a one- or two-step kit, according to your experimental setup.

ZAGENO's Real-Time PCR Troubleshoot will help guide you through any diffculties encountered.

Compare Real-Time PCR Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

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