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Detection Kits

WesternBright™ Sirius™ HRP Substrate SKU: K-12043

Advansta

From
$ 68.60 (1 Kit)
Sizes
3 (1 Kit)
Catalog IDs
K-12043-C20, K-12043-D10, ...
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Secondary Antibodies

Fluorescent Secondary Antibodies SKU: R-05051

Advansta

From
$ 53.90 (1 Unit)
Sizes
2 (1 Unit)
Catalog IDs
R-05051-050, R-05051-250
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Secondary Antibodies

HRP-Conjugated Secondary Antibodies SKU: R-05072

Advansta

From
$ 93.10 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
R-05072-500
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Secondary Antibodies

HRP-Conjugated Secondary Antibodies SKU: R-05075

Advansta

From
$ 93.10 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
R-05075-500
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Secondary Antibodies

Rabbit anti-Mouse IgG1 Secondary Antibody 2213C [Unconjugated] SKU: MAB9627

R&D Systems

From
$ 279.00 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
MAB9627-100
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Isotype Controls

Guinea Pig Guinea Pig IgG, Fab2 Isotype Control SKU: NBP1-97021

Novus Biologicals

From
$ 299.00 (1 mg)
Sizes
1 (1 mg)
Catalog IDs
NBP1-97021-1mg
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Detection Kits

Sulfate Colorimetric Assay Kit SKU: KA1621

Novus Biologicals

From
$ 529.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
KA1621
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ELISA Kits

Human, Mouse, Rat p62/SQSTM1 ELISA Kit Colorimetric SKU: NBP2-61300

Novus Biologicals

From
$ 699.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
NBP2-61300
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Secondary Antibodies

Goat anti-Rat IgG ImmPRESSTM Secondary Antibody [AP Polymer] SKU: MP-5404-NB

Vector Laboratories

From
$ 179.00 (15 ml)
Sizes
1 (15 ml)
Catalog IDs
MP-5404-NB
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Secondary Antibodies

Horse anti-Mouse IgG ImmPRESSTM Secondary Antibody [HRP Polymer] Rat Absorbed SKU: MP-7422-NB

Vector Laboratories

From
$ 279.00 (15 ml)
Sizes
1 (15 ml)
Catalog IDs
MP-7422-NB
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Secondary Antibodies

Goat anti-Rat IgG ImmPRESSTM Secondary Antibody [HRP Polymer] Mouse Absorbed SKU: MP-7444-NB

Vector Laboratories

From
$ 279.00 (15 ml)
Sizes
1 (15 ml)
Catalog IDs
MP-7444-NB
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ELISA Kits

FlexLISA HRP Sandwich ELISA Optimization Kit SKU: 4200-0010

Novus Biologicals

From
$ 679.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
4200-0010
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Identifying a particular protein or fragment of nucleic acid can be accurately achieved by one of two methods.

Antibodies and antibody-related methods (e.g. ELISA or Western Blotting) use immunoglobulins to accurately bind a peptide with a unique sequence, within a protein solution or lysate.

Alternatively, products such as pathogen detection kits use PCR to identify a specific sequence exclusive to a particular bacterium, virus or fungi.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Antibodies

An antibody (aka immunoglobulin) is a glycoprotein produced and secreted by plasma cells. Antibodies play a vital role in regulating the immune system as they can accurately identify particular molecules originating from pathogens and toxins. Each antibody is specified to recognize a specific molecule, referred to as an antigen. Once an antibody has identified its corresponding antigen, it binds to it, which activates the immune response to neutralize and eliminate the invading pathogen.

Detection

Detection tests for the presence and concentration of a molecule in a sample, whether it is a nucleic acid, peptide, antibody, or antigen. Typical methods include immunoassays, the use of fluorophores and microarrays.

(Pathogen) Detection kits

Detection methods may vary depending on the nature of your sample (e.g. RNA, DNA, protein or tissue) and the source of the molecule, i.e. the organism. Specific kits are adapted to unique sample types, from an array of tissues and bodily fluids, including; urine, cell culture, blood, and food.

As an example, detection of a plant-based viroid differs in technique and equipment to that required aimed at testing for the presence of E.coli in a water sample. Fortunately, pathogen detection kits now exist with dedicated technology for identifying a certain bacterium or virus.

Protein Quantification

Protein quantification verifies peptide concentration; it is an essential step before downstream analyses such as Western blotting. Only small loading volumes are required. However, it is recommended to perform quantification before protein purification to reduce variability in further experiments.

Immunodetection

Immunodetection Kits help determine which specific proteins are within a sample via the use of specific complementary antibodies. The two most commonly used techniques are Western blotting and ELISA; both analyze proteins (either qualitatively or quantitatively); utilizing serum, plasma, or tissues. ELISA is simpler and quicker than Western blotting but requires highly specific antibodies. ELISA also mainly works on native proteins, while Westerns works on denatured proteins (and identifies the purity of your proteins).

Molecular Labeling

For processes such as ELISA, molecular labeling is necessary to determine the success of the experiment. Additionally, labeling nucleic acids and proteins can identify their position within the cell or the genome, their presence and concentration can also be determined.