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PIEZO2 Antibody SKU: NBP1-89892
Host
Rabbit
Rabbit
Reactivity
Human
Human
Applicable Processes
Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Scientific Score
6.88
Brand
Novus Biologicals
Sizes
1 (0.1 ml)
Available From
1 Vendor
Price
$ 441.87
Catalog IDs
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PIF1 Antibody [Alexa Fluor 405] SKU: NBP1-97931AF405
Host
Rabbit
Rabbit
Reactivity
Murine
Murine
Applicable Processes
Immunohistochemistry
Immunohistochemistry
Scientific Score
6.19
Brand
Novus Biologicals
Sizes
1 (100 Tests)
Available From
1 Vendor
Price
$ 358.28
Catalog IDs
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PIF1 Antibody [Alexa Fluor 488] SKU: NBP1-97931AF488
Host
Rabbit
Rabbit
Reactivity
Murine
Murine
Applicable Processes
Immunohistochemistry
Immunohistochemistry
Scientific Score
6.19
Brand
Novus Biologicals
Sizes
1 (100 Tests)
Available From
1 Vendor
Price
$ 358.28
Catalog IDs
Added to comparison remove item
PIF1 Antibody [Alexa Fluor 647] SKU: NBP1-97931AF647
Host
Rabbit
Rabbit
Reactivity
Murine
Murine
Applicable Processes
Immunohistochemistry
Immunohistochemistry
Scientific Score
6.19
Brand
Novus Biologicals
Sizes
1 (100 Tests)
Available From
1 Vendor
Price
$ 358.28
Catalog IDs
Added to comparison remove item
PIF1 Antibody [Alexa Fluor 700] SKU: NBP1-97931AF700
Host
Rabbit
Rabbit
Reactivity
Murine
Murine
Applicable Processes
Immunohistochemistry
Immunohistochemistry
Scientific Score
6.19
Brand
Novus Biologicals
Sizes
1 (100 Tests)
Available From
1 Vendor
Price
$ 358.28
Catalog IDs
Added to comparison remove item
PIF1 Antibody [DyLight 405] SKU: NBP1-97931V
Host
Rabbit
Rabbit
Reactivity
Murine
Murine
Applicable Processes
Immunohistochemistry
Immunohistochemistry
Scientific Score
6.78
Brand
Novus Biologicals
Sizes
1 (0.1 ml)
Available From
1 Vendor
Price
$ 358.28
Catalog IDs
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PIF1 Antibody [FITC] SKU: NBP1-97931F
Host
Rabbit
Rabbit
Reactivity
Murine
Murine
Applicable Processes
Immunohistochemistry
Immunohistochemistry
Scientific Score
6.78
Brand
Novus Biologicals
Sizes
1 (0.1 ml)
Available From
1 Vendor
Price
$ 358.28
Catalog IDs
Added to comparison remove item
PIST Antibody SKU: NBP1-88222
Host
/
Reactivity
/
Applicable Processes
/
Scientific Score
2.95
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 441.87
Catalog IDs
Added to comparison remove item
PITX1 Antibody SKU: NBP1-88644
Host
Rabbit
Rabbit
Reactivity
Human
Human
Applicable Processes
Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Immunocytochemistry, Immunofluorescence, Immunohistochemistry
Scientific Score
6.88
Brand
Novus Biologicals
Sizes
1 (0.1 ml)
Available From
1 Vendor
Price
$ 441.87
Catalog IDs
Added to comparison remove item
PKHD1 Antibody SKU: NBP1-89003
Host
/
Reactivity
/
Applicable Processes
/
Scientific Score
2.95
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 441.87
Catalog IDs
Added to comparison remove item
PKLR Antibody (1E3) SKU: NBP1-97937
Host
Murine
Murine
Reactivity
Human
Human
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Scientific Score
7.53
Brand
Novus Biologicals
Sizes
1 (100 µl)
Available From
1 Vendor
Price
$ 286.42
Catalog IDs
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Plakophilin-3 Antibody (23E3/4) [DyLight 350] SKU: NBP1-97674UV
Host
Murine
Murine
Reactivity
Canine, Human, Murine
Canine, Human, Murine
Applicable Processes
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western blot
Scientific Score
6.88
Brand
Novus Biologicals
Sizes
1 (100 Tests)
Available From
1 Vendor
Price
$ 512.27
Catalog IDs
Added to comparison remove item

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Identifying a particular protein or fragment of nucleic acid can be accurately achieved by one of two methods.

Antibodies and antibody-related methods (e.g. ELISA or Western Blotting) use immunoglobulins to accurately bind a peptide with a unique sequence, within a protein solution or lysate.

Alternatively, products such as pathogen detection kits use PCR to identify a specific sequence exclusive to a particular bacterium, virus or fungi.

Antibodies

An antibody (aka immunoglobulin) is a glycoprotein produced and secreted by plasma cells. Antibodies play a vital role in regulating the immune system as they can accurately identify particular molecules originating from pathogens and toxins. Each antibody is specified to recognize a specific molecule, referred to as an antigen. Once an antibody has identified its corresponding antigen, it binds to it, which activates the immune response to neutralize and eliminate the invading pathogen.

Detection

Detection tests for the presence and concentration of a molecule in a sample, whether it is a nucleic acid, peptide, antibody, or antigen. Typical methods include immunoassays, the use of fluorophores and microarrays.

(Pathogen) Detection kits

Detection methods may vary depending on the nature of your sample (e.g. RNA, DNA, protein or tissue) and the source of the molecule, i.e. the organism. Specific kits are adapted to unique sample types, from an array of tissues and bodily fluids, including; urine, cell culture, blood, and food.

As an example, detection of a plant-based viroid differs in technique and equipment to that required aimed at testing for the presence of E.coli in a water sample. Fortunately, pathogen detection kits now exist with dedicated technology for identifying a certain bacterium or virus.

Protein Quantification

Protein quantification verifies peptide concentration; it is an essential step before downstream analyses such as Western blotting. Only small loading volumes are required. However, it is recommended to perform quantification before protein purification to reduce variability in further experiments.

Immunodetection

Immunodetection Kits help determine which specific proteins are within a sample via the use of specific complementary antibodies. The two most commonly used techniques are Western blotting and ELISA; both analyze proteins (either qualitatively or quantitatively); utilizing serum, plasma, or tissues. ELISA is simpler and quicker than Western blotting but requires highly specific antibodies. ELISA also mainly works on native proteins, while Westerns works on denatured proteins (and identifies the purity of your proteins).

Molecular Labeling

For processes such as ELISA, molecular labeling is necessary to determine the success of the experiment. Additionally, labeling nucleic acids and proteins can identify their position within the cell or the genome, their presence and concentration can also be determined.