Customer Service +1 617 855 5944 +1 617 855 5944
Purified Rabbit IgG, Unconjugated
Applicable Processes
/
Isotype
IgG
IgG
Reactivity
Rabbit
Rabbit
Scientific Score
3.62
Brand
Bethyl Laboratories
Sizes
3 (10000 - 100000 µg)
Available From
1 Supplier
Price Range
$ 63.00 - $ 230.00
Added to comparison remove item
Brand
Bethyl Laboratories
Sizes
1 (1000 µg)
Available From
1 Supplier
Price
$ 140.00
Catalog IDs
Added to comparison remove item
anti-IgG control-antibody - FNab04182
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Isotype
IgG
IgG
Reactivity
/
Scientific Score
7.02
Brand
FineTest
Sizes
1 (100 µg)
Available From
1 Supplier
Price
$ 245.00
Catalog IDs
Added to comparison remove item
Ultra-LEAF™ Purified Human IgG4 Isotype Control Recombinant Antibody Clone: QA16A15
Applicable Processes
ELISA, Flow cytometry
ELISA, Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
7.82
Brand
BioLegend
Sizes
2 (100 - 1000 µg)
Available From
1 Supplier
Price Range
$ 225.00 - $ 450.00
Catalog IDs
Added to comparison remove item
Normal Rabbit IgG-FITC SKU: 10010322
Applicable Processes
/
Isotype
/
Reactivity
/
Scientific Score
1.49
Brand
Cayman Chemical
Sizes
1 (1 Each)
Available From
1 Supplier
Price
$ 114.70
Catalog IDs
Added to comparison remove item
IgG1 Isotype Control FITC Monoclonal Antibody (Clone MOPC-21) SKU: 10343
Applicable Processes
/
Isotype
/
Reactivity
/
Scientific Score
1.49
Brand
Cayman Chemical
Sizes
2 (1 - 100 Each)
Available From
1 Supplier
Price
$ 139.79
Catalog IDs
Added to comparison remove item
Swine IgG-UNLB
Applicable Processes
ELISA, Flow cytometry
ELISA, Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
6.38
Brand
Southern Biotechnology
Sizes
1 (10000 µg)
Available From
1 Supplier
Price
$ 88.92
Catalog IDs
Added to comparison remove item
Mouse IgG2c-UNLB
Applicable Processes
ELISA, Flow cytometry
ELISA, Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
7.94
Brand
Southern Biotechnology
Sizes
1 (1000 µg)
Available From
1 Supplier
Price
$ 353.08
Catalog IDs
Added to comparison remove item
Rat IgG1-APC/CY5.5
Applicable Processes
Flow cytometry
Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
6.79
Brand
Southern Biotechnology
Sizes
1 (100 µg)
Available From
1 Supplier
Price
$ 215.77
Catalog IDs
Added to comparison remove item
Rat IgG1-APC/CY7
Applicable Processes
Flow cytometry
Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
6.79
Brand
Southern Biotechnology
Sizes
1 (100 µg)
Available From
1 Supplier
Price
$ 222.31
Catalog IDs
Added to comparison remove item
Rat IgG1-PE/CY5.5
Applicable Processes
Flow cytometry
Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
6.79
Brand
Southern Biotechnology
Sizes
1 (100 µg)
Available From
1 Supplier
Price
$ 209.23
Catalog IDs
Added to comparison remove item
Rat IgG2a-APC/CY5.5
Applicable Processes
Flow cytometry
Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
6.79
Brand
Southern Biotechnology
Sizes
1 (100 µg)
Available From
1 Supplier
Price
$ 215.77
Catalog IDs
Added to comparison remove item

Can't find what you are looking for? Request the product now and we'll find it for you (typically within 1 hour).

Request Product

Isotype Control Function & Applications

Immunoassay techniques such as Flow Cytometry, ELISA or Western Blot provide scientists with an excellent way to detect whether a sample contains a target particle, like protein, peptide or hormone. This is achieved thanks to antibodies ability to bind to antigens. This hook-like mechanism allows for scientists to 'fish out' whatever particle they desire. However, it is important to set up controls in such experiments, as antibodies can bind to other undesired molecules. Thus it is important to control such nonspecific binding, to ensure that your detection experiments run smoothly. This is achieved through isotype controls.

Do note that isotype controls are primarily used in flow cytometry, but can also be utilized in other applications like western blotting and ELISA.

Negative Controls

Negative controls work by not giving a response, which is to be expected. In not having a result, scientists can have greater confidence that their experiment is working as it should. The isotype control is one such tool, and work by being a copy of the primary antibody used in the test, but lack specificity. That is, an isotype control is a primary antibody with the same Fc-region as the actual experiment but a different variable region, thus it has a non-specific binding region. More specifically, the isotype control antibody should be the same isotype of heavy chain and light chain, the same fluorchrome and F:P ratio. This enables scientists to confirm the specificity of binding in the experiment. Simply put, to show that the used antibody is specific, these isotype controls should not result in similar staining or band patterns.