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Isotype Controls

Score 5.98

IgG1-APC Isotype Control murine SKU: SAB4700724

Sigma-Aldrich

Applicable Processes
/
/
Isotype
/
/
Concentration
0.1 mg/ml
0.1 mg/ml
From
$ 323.00 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
SAB4700724-100UG
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Isotype Controls

Score 5.98

IgM-FITC Isotype Control murine SKU: SAB4700699

Sigma-Aldrich

Applicable Processes
/
/
Isotype
/
/
Concentration
1 mg/ml
1 mg/ml
From
$ 260.00 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
SAB4700699-100UG
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Isotype Controls

Score 4.12

IgG−FITC from human serum SKU : f9636

Sigma-Aldrich

Applicable Processes
/
/
Isotype
/
/
Concentration
~20 mg/mL
~20 mg/mL
From
$ 139.00 (1 ml)
Sizes
1 (1 ml)
Catalog IDs
F9636-1ML
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Isotype Controls

Score 4.27

IgG1, Kappa from murine myeloma SKU : M9269

Sigma-Aldrich

Applicable Processes
/
/
Isotype
/
/
Concentration
/
/
From
$ 191.00 (1 mg)
Sizes
1 (1 mg)
Catalog IDs
M9269-1MG
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Isotype Controls

Score 4.26

IgG1 Isotype Control from murine myeloma SKU : M5284

Sigma-Aldrich

Applicable Processes
/
/
Isotype
/
/
Concentration
/
/
From
$ 102.00 (0.1 mg)
Sizes
1 (0.1 mg)
Catalog IDs
M5284-.1MG
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Isotype Controls

Score 4.46

Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 SKU: 60070AZ

STEMCELL Technologies

Applicable Processes
/
/
Isotype
IgG
IgG
Concentration
/
/
From
$ 41.00 (25 µg)
Sizes
2 (25 - 100 µg)
Catalog IDs
60070AZ.1, 60070AZ
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Isotype Controls

Score 5.98

Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 SKU: 60072PE

STEMCELL Technologies

Applicable Processes
/
/
Isotype
IgG
IgG
Concentration
/
/
From
$ 47.00 (25 µg)
Sizes
2 (25 - 100 µg)
Catalog IDs
60072PE.1, 60072PE
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Isotype Controls

Score 4.46

Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 SKU: 60076PE

STEMCELL Technologies

Applicable Processes
/
/
Isotype
IgG
IgG
Concentration
/
/
From
$ 55.00 (25 µg)
Sizes
2 (25 - 100 µg)
Catalog IDs
60076PE.1, 60076PE
Added to comparison remove item

Isotype Controls

Score 5.98

Rat IgG2a, kappa Isotype Control Antibody, Clone RTK2758 SKU: 60076AZ

STEMCELL Technologies

Applicable Processes
/
/
Isotype
IgG
IgG
Concentration
/
/
From
$ 61.00 (25 µg)
Sizes
2 (25 - 100 µg)
Catalog IDs
60076AZ.1, 60076AZ
Added to comparison remove item

Isotype Controls

Score 4.46

Mouse IgG1, kappa Isotype Control Antibody, Clone MOPC-21 SKU: 60070FI

STEMCELL Technologies

Applicable Processes
/
/
Isotype
IgG
IgG
Concentration
/
/
From
$ 34.00 (50 µg)
Sizes
2 (50 - 200 µg)
Catalog IDs
60070FI.1, 60070FI
Added to comparison remove item

Isotype Controls

Score 4.46

Mouse IgG2a, kappa Isotype Control Antibody, Clone MOPC-173 SKU: 60071

STEMCELL Technologies

Applicable Processes
/
/
Isotype
IgG
IgG
Concentration
/
/
From
$ 35.00 (50 µg)
Sizes
2 (50 - 500 µg)
Catalog IDs
60071.1, 60071
Added to comparison remove item

Isotype Controls

Score 5.98

Mouse IgG2b, kappa Isotype Control Antibody, Clone MPC-11 SKU: 60072

STEMCELL Technologies

Applicable Processes
/
/
Isotype
IgG
IgG
Concentration
/
/
From
$ 35.00 (50 µg)
Sizes
2 (50 - 500 µg)
Catalog IDs
60072.1, 60072
Added to comparison remove item

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Isotype Control Function & Applications

Immunoassay techniques such as Flow Cytometry, ELISA or Western Blot provide scientists with an excellent way to detect whether a sample contains a target particle, like protein, peptide or hormone. This is achieved thanks to antibodies ability to bind to antigens. This hook-like mechanism allows for scientists to 'fish out' whatever particle they desire. However, it is important to set up controls in such experiments, as antibodies can bind to other undesired molecules. Thus it is important to control such nonspecific binding, to ensure that your detection experiments run smoothly. This is achieved through isotype controls.

Do note that isotype controls are primarily used in flow cytometry, but can also be utilized in other applications like western blotting and ELISA.

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Negative Controls

Negative controls work by not giving a response, which is to be expected. In not having a result, scientists can have greater confidence that their experiment is working as it should. The isotype control is one such tool, and work by being a copy of the primary antibody used in the test, but lack specificity. That is, an isotype control is a primary antibody with the same Fc-region as the actual experiment but a different variable region, thus it has a non-specific binding region. More specifically, the isotype control antibody should be the same isotype of heavy chain and light chain, the same fluorchrome and F:P ratio. This enables scientists to confirm the specificity of binding in the experiment. Simply put, to show that the used antibody is specific, these isotype controls should not result in similar staining or band patterns.