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Isotype Controls on ZAGENO

Pacific Blue™ Mouse IgG1, κ Isotype Ctrl Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From $ 175.00 (100 µg)
Sizes 3 (100 - 500 µg)
Catalog IDs 400131, 400151, ...
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PerCP/Cy5.5 Mouse IgM, κ Isotype Ctrl Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From Not available
Sizes 2 (25 - 100 Reactions)
Catalog IDs 401623, 401624
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PE Mouse IgG1, κ Isotype Ctrl (FC) Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From $ 110.00 (100 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs 400113, 400114
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PE Mouse IgG1, κ Isotype Ctrl (ICFC) Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From Not available
Sizes 2 (25 - 100 Reactions)
Catalog IDs 400139, 400140
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APC Rat IgG2a, κ Isotype Ctrl Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From Not available
Sizes 2 (25 - 100 µg)
Catalog IDs 400511, 400512
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Alexa Fluor® 488 Mouse IgG1, κ Isotype Ctrl (FC) Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From $ 175.00 (100 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs 400132, 400129
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Alexa Fluor® 488 Rat IgG2b, κ Isotype Ctrl Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From $ 150.00 (100 µg)
Sizes 1 (100 µg)
Catalog IDs 400625
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APC Mouse IgG1, κ Isotype Ctrl (FC) Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From $ 140.00 (100 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs 400121, 400122
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Brilliant Violet 421™ Mouse IgG2a, κ Isotype Ctrl Antibody BioLegend
Reagent Type Antibodies Applicable Processes / Quantity /
From Not available
Sizes 2 (25 - 100 Reactions)
Catalog IDs 400259, 400260
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IgG−FITC from human serum SKU : f9636 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 119.50 (1 ml)
Sizes 1 (1 ml)
Catalog IDs F9636-1ML
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IgG1-APC Isotype Control murine SKU: SAB4700724 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 314.00 (100 µg)
Sizes 1 (100 µg)
Catalog IDs SAB4700724-100UG
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IgM-FITC Isotype Control murine SKU: SAB4700699 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 252.50 (100 µg)
Sizes 1 (100 µg)
Catalog IDs SAB4700699-100UG
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Isotype Control Function & Applications

Immunoassay techniques such as Flow Cytometry, ELISA or Western Blot provide scientists with an excellent way to detect whether a sample contains a target particle, like protein, peptide or hormone. This is achieved thanks to antibodies ability to bind to antigens. This hook-like mechanism allows for scientists to 'fish out' whatever particle they desire. However, it is important to set up controls in such experiments, as antibodies can bind to other undesired molecules. Thus it is important to control such nonspecific binding, to ensure that your detection experiments run smoothly. This is achieved through isotype controls.

Do note that isotype controls are primarily used in flow cytometry, but can also be utilized in other applications like western blotting and ELISA.

Negative Controls

Negative controls work by not giving a response, which is to be expected. In not having a result, scientists can have greater confidence that their experiment is working as it should. The isotype control is one such tool, and work by being a copy of the primary antibody used in the test, but lack specificity. That is, an isotype control is a primary antibody with the same Fc-region as the actual experiment but a different variable region, thus it has a non-specific binding region. More specifically, the isotype control antibody should be the same isotype of heavy chain and light chain, the same fluorchrome and F:P ratio. This enables scientists to confirm the specificity of binding in the experiment. Simply put, to show that the used antibody is specific, these isotype controls should not result in similar staining or band patterns.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.