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Isotype Controls on ZAGENO

Mouse IgG1 Isotype Control MP21 SKU: NBP2-21942 Novus Biologicals
From $ 199.00 (0.25 mg)
Sizes 2 (0.25 - 0.5 mg)
Catalog IDs NBP2-21942-0.25mg, NBP2-21942-0.5mg
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Rat IgG1 Isotype Control HPN33 SKU: NBP2-21946 Novus Biologicals
From $ 199.00 (0.25 mg)
Sizes 2 (0.25 - 0.5 mg)
Catalog IDs NBP2-21946-0.25mg, NBP2-21946-0.5mg
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Human IgG3 Isotype Control SKU: DDXCH03P-100 Dendritics
From $ 179.00 (0.1 mg)
Sizes 1 (0.1 mg)
Catalog IDs DDXCH03P-100
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Rat IgG1 Isotype Control SKU: DDXCR01 Dendritics
From $ 179.00 (0.1 mg)
Sizes 1 (0.1 mg)
Catalog IDs DDXCR01
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Rat IgM Isotype Control SKU: DDXCR05 Dendritics
From $ 179.00 (0.1 mg)
Sizes 1 (0.1 mg)
Catalog IDs DDXCR05
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Mouse IgG2A Flow Cytometry/IHC Isotype Control (Cl 133304) R&D Systems
From $ 175.00 (500 µg)
Sizes 1 (500 µg)
Catalog IDs MAB0031
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Rat IgG1 Isotype Control (Clone 43414R) R&D Systems
Applicable Processes /
From $ 199.00 (500 μg)
Sizes 1 (500 μg)
Catalog IDs MAB005R
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Rat IgG2B Isotype Control (Clone 141945) R&D Systems
From $ 175.00 (500 µg)
Sizes 1 (500 µg)
Catalog IDs MAB0061
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Purified Armenian Hamster IgM Isotype Ctrl Antibody Clone: HTK204 BioLegend
Applicable Processes /
From $ 130.00 (500 µg)
Sizes 1 (500 µg)
Catalog IDs 401002
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Purified Mouse IgG3, κ Isotype Ctrl Antibody Clone: MG3-35 BioLegend
Applicable Processes /
From $ 40.00 (50 µg)
Sizes 2 (50 - 500 µg)
Catalog IDs 401301, 401302
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Purified Mouse IgM, λ Isotype Ctrl Antibody Clone: MOPC-104E BioLegend
Applicable Processes /
From $ 45.00 (50 µg)
Sizes 2 (50 - 500 µg)
Catalog IDs 401101, 401102
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Purified Rat IgG2a, κ Isotype Ctrl Antibody Clone: RTK2758 BioLegend
Applicable Processes /
From $ 45.00 (50 µg)
Sizes 2 (50 - 500 µg)
Catalog IDs 400501, 400502
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Isotype Control Function & Applications

Immunoassay techniques such as Flow Cytometry, ELISA or Western Blot provide scientists with an excellent way to detect whether a sample contains a target particle, like protein, peptide or hormone. This is achieved thanks to antibodies ability to bind to antigens. This hook-like mechanism allows for scientists to 'fish out' whatever particle they desire. However, it is important to set up controls in such experiments, as antibodies can bind to other undesired molecules. Thus it is important to control such nonspecific binding, to ensure that your detection experiments run smoothly. This is achieved through isotype controls.

Do note that isotype controls are primarily used in flow cytometry, but can also be utilized in other applications like western blotting and ELISA.

Negative Controls

Negative controls work by not giving a response, which is to be expected. In not having a result, scientists can have greater confidence that their experiment is working as it should. The isotype control is one such tool, and work by being a copy of the primary antibody used in the test, but lack specificity. That is, an isotype control is a primary antibody with the same Fc-region as the actual experiment but a different variable region, thus it has a non-specific binding region. More specifically, the isotype control antibody should be the same isotype of heavy chain and light chain, the same fluorchrome and F:P ratio. This enables scientists to confirm the specificity of binding in the experiment. Simply put, to show that the used antibody is specific, these isotype controls should not result in similar staining or band patterns.

Problems in the lab? Learn about methods and experiments in our Knowledge section or look up a specific Troubleshooting guide.