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IgG control Antibody SKU: abx234182
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Isotype
IgG
IgG
Reactivity
/
Scientific Score
9.17
Brand
abbexa
Sizes
1 (100 µg)
Available From
1 Vendor
Price
$ 449.50
Catalog IDs
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IgG light chain Antibody SKU: abx140241
Applicable Processes
Flow cytometry, Immunohistochemistry, Western blot
Flow cytometry, Immunohistochemistry, Western blot
Isotype
IgG
IgG
Reactivity
Cow
Cow
Scientific Score
9.28
Brand
abbexa
Sizes
1 (0.1 mg)
Available From
1 Vendor
Price
$ 319.00
Catalog IDs
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IgG-Fc Antibody SKU: abx137072
Applicable Processes
/
Isotype
/
Reactivity
Human
Human
Scientific Score
7.94
Brand
abbexa
Sizes
2 (0.5 - 1 mg)
Available From
1 Vendor
Price Range
$ 319.00 - $ 493.00
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Purified Rabbit IgG, Unconjugated
Applicable Processes
/
Isotype
IgG
IgG
Reactivity
Rabbit
Rabbit
Scientific Score
3.62
Brand
Bethyl Laboratories
Sizes
3 (10 - 100 mg)
Available From
1 Vendor
Price Range
$ 63.00 - $ 230.00
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Brand
Bethyl Laboratories
Sizes
1 (1 mg)
Available From
1 Vendor
Price
$ 140.00
Catalog IDs
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anti-IgG control-antibody - FNab04182
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Isotype
IgG
IgG
Reactivity
/
Scientific Score
7.02
Brand
FineTest
Sizes
1 (100 µg)
Available From
1 Vendor
Price
$ 245.00
Catalog IDs
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Normal Rabbit IgG-FITC SKU: 10010322
Scientific Score
1.49
Brand
Cayman Chemical
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 120.32
Catalog IDs
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Brand
Cayman Chemical
Sizes
2 (1 - 100 Pack)
Available From
1 Vendor
Price
$ 146.64
Catalog IDs
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Chicken IgY-HRP
Applicable Processes
ELISA, Immunocytochemistry
ELISA, Immunocytochemistry
Isotype
IgY
IgY
Reactivity
/
Scientific Score
6.38
Brand
Southern Biotechnology
Sizes
1 (1 ml)
Available From
1 Vendor
Price
$ 98.00
Catalog IDs
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Chicken IgY-UNLB
Applicable Processes
ELISA, Flow cytometry
ELISA, Flow cytometry
Isotype
IgY
IgY
Reactivity
/
Scientific Score
5.69
Brand
Southern Biotechnology
Sizes
1 (10 mg)
Available From
1 Vendor
Price
$ 140.87
Catalog IDs
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Donkey IgG-UNLB
Applicable Processes
ELISA, Flow cytometry
ELISA, Flow cytometry
Isotype
IgG
IgG
Reactivity
/
Scientific Score
6.38
Brand
Southern Biotechnology
Sizes
1 (10 mg)
Available From
1 Vendor
Price
$ 63.69
Catalog IDs
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Mouse Immunoglobulin Panel
Scientific Score
3.55
Brand
Southern Biotechnology
Sizes
1 (1 Kit)
Available From
1 Vendor
Price
$ 202.12
Catalog IDs
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Isotype Control Function & Applications

Immunoassay techniques such as Flow Cytometry, ELISA or Western Blot provide scientists with an excellent way to detect whether a sample contains a target particle, like protein, peptide or hormone. This is achieved thanks to antibodies ability to bind to antigens. This hook-like mechanism allows for scientists to 'fish out' whatever particle they desire. However, it is important to set up controls in such experiments, as antibodies can bind to other undesired molecules. Thus it is important to control such nonspecific binding, to ensure that your detection experiments run smoothly. This is achieved through isotype controls.

Do note that isotype controls are primarily used in flow cytometry, but can also be utilized in other applications like western blotting and ELISA.

Negative Controls

Negative controls work by not giving a response, which is to be expected. In not having a result, scientists can have greater confidence that their experiment is working as it should. The isotype control is one such tool, and work by being a copy of the primary antibody used in the test, but lack specificity. That is, an isotype control is a primary antibody with the same Fc-region as the actual experiment but a different variable region, thus it has a non-specific binding region. More specifically, the isotype control antibody should be the same isotype of heavy chain and light chain, the same fluorchrome and F:P ratio. This enables scientists to confirm the specificity of binding in the experiment. Simply put, to show that the used antibody is specific, these isotype controls should not result in similar staining or band patterns.