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Primary Antibodies

BV711 Hamster Anti-Mouse CD29 Clone HM β1-1 RUO

BD

Host
Hamster
Hamster
Reactivity
Murine
Murine
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
740708
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Primary Antibodies

BV711 Hamster Anti-Mouse CD49e Clone HM alpha 5-1 RUO

BD

Host
Hamster
Hamster
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
743113
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Primary Antibodies

BV711 Hamster Anti-Rat CD62L Clone HRL1 RUO

BD

Host
Hamster
Hamster
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
743151
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Primary Antibodies

BV711 Mouse Anti-Mouse CD22.2 Clone Cy34.1 RUO

BD

Host
Murine
Murine
Reactivity
Murine
Murine
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
740682
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Primary Antibodies

BV711 Mouse Anti-Mouse CD244.2 Clone 2B4 RUO

BD

Host
Murine
Murine
Reactivity
Murine
Murine
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
740671
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Primary Antibodies

BV711 Mouse Anti-Mouse H-2D[q]/H-2L[q] Clone KH117 RUO

BD

Host
Mouse
Mouse
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
745487
Added to comparison remove item

Primary Antibodies

BV711 Mouse Anti-Mouse H-2Kk Clone AF3-12.1 RUO

BD

Host
Mouse
Mouse
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
743938
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Primary Antibodies

BV711 Mouse Anti-Mouse I-A[d] Clone 34-5-3 RUO

BD

Host
Mouse
Mouse
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
745493
Added to comparison remove item

Primary Antibodies

BV711 Mouse Anti-Mouse I-A[p] Clone 7-16.17 RUO

BD

Host
Mouse
Mouse
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
745435
Added to comparison remove item

Primary Antibodies

BV711 Mouse Anti-Mouse I-E[k] Clone 14-4-4S RUO

BD

Host
Mouse
Mouse
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
745465
Added to comparison remove item

Primary Antibodies

BV711 Mouse Anti-Mouse I-E[κ] Clone 17-3-3 RUO

BD

Host
Mouse
Mouse
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
745483
Added to comparison remove item

Primary Antibodies

BV711 Mouse Anti-Mouse IgG1[a] Clone 10.9 RUO

BD

Host
Mouse
Mouse
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry
Flow cytometry
From
$ 350.00 (50 µg)
Sizes
1 (50 µg)
Catalog IDs
743263
Added to comparison remove item

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Primary Antibody Structure & Function

Primary antibodies, a subgroup of immunoglobulins known as glycoproteins, specifically bind to one antigen or protein with high affinity. This is not to be confused with a secondary antibody, which binds to another (primary) antibody that is linked to another molecule.

Primary antibodies are extremely useful for detecting disease biomarkers in cancer, diabetes, Parkinson′s and Alzheimer′s disease. One example of such uses is with Pharmacokinetics, the study of how an organism affects a drug (e.g. digestion and metabolization). Here, antibodies are used for ADME (absorption, distribution, metabolism, and excretion) and testing multi-drug resistance (MDR).

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Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Monoclonal and Polyclonal Antibodies

Mice, rats, and goats are used to produce primary antibodies, which are either polyclonal (pAb) or monoclonal (mAb). Polyclonal antibodies are derived from different B-cell lineages, thus producing a mixture of antibodies with varying levels of affinity which target multiple epitopes. Monoclonal antibodies are sourced from one B-cell lineage, thus producing highly specific antibodies with only one antibody subtype.

Use in Cell Biology

Primary antibodies are key components in cell biology for processes such as:

  • apoptosis

  • cell stress

  • cell cycle progression

  • cell signaling

  • cytoskeleton and extracellular matrix (ECM) organization

  • gene expression

  • metabolism

  • protein synthesis and degradation

  • enzyme phenotype profiling

  • ion channel transport

Use in Gene Expression

Primary antibodies are useful in monitoring gene expression as part of detection systems which use a broad range of markers including b-galactosidase, biotin, digoxin, and DNP.

Other systems make use of protein fusion tags. These can consist of: alkaline phosphatase, FLAG, glutathione-S-transferase (GST), hemagglutinin (HA), maltose binding protein (MBP), c-myc, polyhistidine, thioredoxin, and peroxidase.

Primary antibodies can also monitor changes in phenotypes and status of the proteasome under normal and diseased conditions.

Leukocyte antigen and cytokine expression can also be measured; CD antigens (a type of leukocyte antigen) can help distinguish cell lineage, and functional subsets including:

  • integrins

  • selectins

  • human leukocyte (HLA) antigens (histocompatibility antigens (glycoproteins)

  • interferons

  • interleukins

  • tumor necrosis factor (TNF)

Concentration and Modification Measurement

Aside from detection, primary antibodies can also be used to measure concentration changes as well as specific modifications such as phosphorylation, methylation, or glycosylation. This allows for the study of proteins which are activated, silenced, processed or causing disease.