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Goat anti-Rabbit IgG-Fc Fragment Antibody HRP Conjugated - A120-111P
Reactivity
Rabbit
Rabbit
Applicable Processes
ELISA, Immunocytochemistry, Immunohistochemistry, Western blot
ELISA, Immunocytochemistry, Immunohistochemistry, Western blot
Conjugate Type
HRP (Horseradish Peroxidase)
HRP (Horseradish Peroxidase)
Scientific Score
10.00
Brand
Bethyl Laboratories
Sizes
1 (1000 µl)
Available From
1 Supplier
Price
$ 136.00
Catalog IDs
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anti-Human IgG-antibody - FNab04077
Reactivity
Human
Human
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Conjugate Type
/
Scientific Score
7.53
Brand
FineTest
Sizes
1 (100 µg)
Available From
1 Supplier
Price
$ 300.00
Catalog IDs
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Goat Anti-Human IgG, Fcγ Fragment Specific (Adsorbed Against: Ms., Bv., Hs.) - HRPO Adsorbed Against: - H605
Reactivity
Human
Human
Applicable Processes
/
Conjugate Type
HRP (Horseradish Peroxidase)
HRP (Horseradish Peroxidase)
Scientific Score
8.71
Brand
Leinco Technologies
Sizes
1 (800 µg)
Available From
1 Supplier
Price
$ 145.00
Catalog IDs
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Goat Anti-Human Kappa-HRP
Reactivity
Human
Human
Applicable Processes
/
Conjugate Type
HRP (Horseradish Peroxidase)
HRP (Horseradish Peroxidase)
Scientific Score
9.96
Brand
Southern Biotechnology
Sizes
1 (1000 µl)
Available From
2 Suppliers
Price Range
$ 130.00 - $ 151.01
Catalog IDs
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Goat Anti-Mouse Kappa-UNLB
Reactivity
Mouse
Mouse
Applicable Processes
/
Conjugate Type
/
Scientific Score
7.53
Brand
Southern Biotechnology
Sizes
1 (1000 µl)
Available From
2 Suppliers
Price Range
$ 165.00 - $ 191.66
Catalog IDs
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Goat Anti-Mouse Kappa-HRP
Reactivity
Mouse
Mouse
Applicable Processes
/
Conjugate Type
HRP (Horseradish Peroxidase)
HRP (Horseradish Peroxidase)
Scientific Score
7.53
Brand
Southern Biotechnology
Sizes
1 (1000 µl)
Available From
2 Suppliers
Price Range
$ 141.00 - $ 180.04
Catalog IDs
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Goat Anti-Mouse IgG2c, Human ads-UNLB
Reactivity
Mouse
Mouse
Applicable Processes
Dot Blot, ELISA, Flow cytometry, Immunohistochemistry, Western blot
Dot Blot, ELISA, Flow cytometry, Immunohistochemistry, Western blot
Conjugate Type
/
Scientific Score
9.07
Brand
Southern Biotechnology
Sizes
1 (1000 µg)
Available From
3 Suppliers
Price Range
$ 180.00 - $ 235.38
Catalog IDs
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IgG Human (Fab) SKU: 009-0105
Reactivity
/
Applicable Processes
/
Conjugate Type
/
Scientific Score
3.35
Brand
Rockland Immunochemicals
Sizes
1 (2000 µg)
Available From
1 Supplier
Price
$ 171.00
Catalog IDs
Added to comparison remove item
Anti-Mouse IgG - Atto 594 antibody produced in goat SKU : 76085
Reactivity
Mouse
Mouse
Applicable Processes
/
Conjugate Type
/
Scientific Score
6.60
Brand
Sigma-Aldrich
Sizes
1 (1000 µl)
Available From
2 Suppliers
Price Range
$ 238.00 - $ 246.46
Catalog IDs
Added to comparison remove item
Anti-Rabbit IgG - Atto 594 antibody produced in goat SKU : 77671
Reactivity
Rabbit
Rabbit
Applicable Processes
/
Conjugate Type
/
Scientific Score
6.20
Brand
Sigma-Aldrich
Sizes
1 (1000 µl)
Available From
2 Suppliers
Price Range
$ 238.00 - $ 246.46
Catalog IDs
Added to comparison remove item
Anti-Mouse IgG - Atto 633 antibody produced in goat SKU : 78102
Reactivity
Mouse
Mouse
Applicable Processes
/
Conjugate Type
/
Scientific Score
6.20
Brand
Sigma-Aldrich
Sizes
1 (1000 µl)
Available From
2 Suppliers
Price Range
$ 238.00 - $ 246.46
Catalog IDs
Added to comparison remove item
Anti-Rabbit IgG - Atto 655 antibody produced in goat SKU : 78519
Reactivity
Rabbit
Rabbit
Applicable Processes
/
Conjugate Type
/
Scientific Score
6.20
Brand
Sigma-Aldrich
Sizes
1 (1000 µl)
Available From
2 Suppliers
Price Range
$ 238.00 - $ 246.46
Catalog IDs
Added to comparison remove item

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Secondary Antibody Function & Applications

Secondary antibodies bind to primary antibodies (on the Fc region) or fragments of antibodies (Fc or Fab), enabling indirect detection and purification of an antigen. They are essential for immunolabeling in ELISA, Western Blotting, Cellular Imaging and Flow Cytometry.

Targeting Primary Antibodies

These antibodies can target primary antibodies from a particular species, such as rat, known as anti-rat antibodies, allowing one secondary antibody to target multiple primary antibodies. On top of that, they can be specific to a particular class of antibodies such as IgG, or even IgG1.

Antigen Visualization

Some secondary antibodies can amplify the signal via conjugated fluorescent, colorimetric or chemiluminescent probes. Colorimetric assays detect alkaline phosphatase or horseradish peroxidase conjugates, and the streptavidin conjugate binding system enhances the colorimetric signal. Fluorescent assays detect Fluorescein (FITC), Rhodamine, TRITC, Cyanine (Cy3) or Phycoerythrin (R-PE).