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Secondary Antibodies

Score 3.37

Fluorescent Secondary Antibodies SKU: R-05051

Advansta

From
$ 53.90 (1 Unit)
Sizes
2 (1 Unit)
Catalog IDs
R-05051-050, R-05051-250
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Secondary Antibodies

Score 2.30

HRP-Conjugated Secondary Antibodies SKU: R-05072

Advansta

From
$ 93.10 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
R-05072-500
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Secondary Antibodies

Score 2.15

HRP-Conjugated Secondary Antibodies SKU: R-05075

Advansta

From
$ 93.10 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
R-05075-500
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Secondary Antibodies

Score 8.75

Rabbit anti-Mouse IgG1 Secondary Antibody 2213C [Unconjugated] SKU: MAB9627

R&D Systems

From
$ 279.00 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
MAB9627-100
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Secondary Antibodies

Score 7.43

Goat anti-Rat IgG ImmPRESSTM Secondary Antibody [AP Polymer] SKU: MP-5404-NB

Vector Laboratories

From
$ 179.00 (15 ml)
Sizes
1 (15 ml)
Catalog IDs
MP-5404-NB
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Secondary Antibodies

Score 7.43

Horse anti-Mouse IgG ImmPRESSTM Secondary Antibody [HRP Polymer] Rat Absorbed SKU: MP-7422-NB

Vector Laboratories

From
$ 279.00 (15 ml)
Sizes
1 (15 ml)
Catalog IDs
MP-7422-NB
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Secondary Antibodies

Score 7.43

Goat anti-Rat IgG ImmPRESSTM Secondary Antibody [HRP Polymer] Mouse Absorbed SKU: MP-7444-NB

Vector Laboratories

From
$ 279.00 (15 ml)
Sizes
1 (15 ml)
Catalog IDs
MP-7444-NB
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Secondary Antibodies

Score 8.64

Rabbit anti-Porcine IgG H+L Secondary Antibody SKU: NB120-6705

Novus Biologicals

From
$ 229.00 (2 mg)
Sizes
1 (2 mg)
Catalog IDs
NB120-6705
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Secondary Antibodies

Score 8.65

Goat anti-Porcine IgG H+L Secondary Antibody SKU: NBP1-72700

Novus Biologicals

From
$ 249.00 (2 mg)
Sizes
1 (2 mg)
Catalog IDs
NBP1-72700
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Secondary Antibodies

Score 5.67

Goat Anti-human IgM secondary antibody, FITC Conjugated SKU: 10704

Cepham Life Sciences

Applicable Processes
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Reactivity
/
/
Conjugate Type
/
/
From
$ 119.75 (0.25 ml)
Sizes
2 (0.25 - 0.5 ml)
Catalog IDs
10704, 10704-1
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Secondary Antibodies

Score 5.67

Mouse Anti-rabbit IgG secondary antibody, Biotin Conjugated SKU: 10682

Cepham Life Sciences

Applicable Processes
/
/
Reactivity
/
/
Conjugate Type
/
/
From
$ 126.05 (0.5 ml)
Sizes
2 (0.5 - 1 ml)
Catalog IDs
10682, 10682-1
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Secondary Antibodies

Score 5.67

Goat Anti-human IgA secondary antibody, FITC Conjugated SKU: 10703

Cepham Life Sciences

Applicable Processes
/
/
Reactivity
/
/
Conjugate Type
/
/
From
$ 119.75 (0.25 ml)
Sizes
2 (0.25 - 0.5 ml)
Catalog IDs
10703, 10703-1
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Secondary Antibody Function & Applications

Secondary antibodies bind to primary antibodies (on the Fc region) or fragments of antibodies (Fc or Fab), enabling indirect detection and purification of an antigen. They are essential for immunolabeling in ELISA, Western Blotting, Cellular Imaging and Flow Cytometry.

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Targeting Primary Antibodies

These antibodies can target primary antibodies from a particular species, such as rat, known as anti-rat antibodies, allowing one secondary antibody to target multiple primary antibodies. On top of that, they can be specific to a particular class of antibodies such as IgG, or even IgG1.

Antigen Visualization

Some secondary antibodies can amplify the signal via conjugated fluorescent, colorimetric or chemiluminescent probes. Colorimetric assays detect alkaline phosphatase or horseradish peroxidase conjugates, and the streptavidin conjugate binding system enhances the colorimetric signal. Fluorescent assays detect Fluorescein (FITC), Rhodamine, TRITC, Cyanine (Cy3) or Phycoerythrin (R-PE).