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Alexa Fluor® 647 AffiniPure Fab Fragment Goat Anti-Mouse IgG2b, Fcγ fragment specific
Reactivity
Mouse
Mouse
Applicable Processes
/
Conjugate Type
Alexa Fluor
Alexa Fluor
Brand
Jackson ImmunoResearch Laboratories
Sizes
1 (0.5 mg)
Available From
1 Vendor
Price
$ 237.00
Catalog IDs
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Goat Anti-Mouse IgG Antibody (H&L) [FITC], pAb
Reactivity
Mouse
Mouse
Applicable Processes
Flow cytometry, Immunocytochemistry, Immunohistochemistry
Flow cytometry, Immunocytochemistry, Immunohistochemistry
Conjugate Type
FITC (Fluorescein isothiocyanate)
FITC (Fluorescein isothiocyanate)
Scientific Score
9.19
Brand
GenScript
Sizes
1 (1 mg)
Available From
2 Vendors
Price Range
$ 79.00 - $ 85.91
Catalog IDs
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Goat Anti-Rat IgG (H&L) [HRP] Antibody, pAb
Reactivity
Rat
Rat
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Conjugate Type
HRP (Horseradish Peroxidase)
HRP (Horseradish Peroxidase)
Scientific Score
9.19
Brand
GenScript
Sizes
1 (1 mg)
Available From
2 Vendors
Price Range
$ 79.00 - $ 85.91
Catalog IDs
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Goat Anti-Rabbit IgG Antibody (H&L), pAb
Reactivity
Rabbit
Rabbit
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Conjugate Type
/
Scientific Score
9.84
Brand
GenScript
Sizes
1 (1 mg)
Available From
2 Vendors
Price Range
$ 50.00 - $ 54.37
Catalog IDs
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Alexa Fluor® 647 AffiniPure Fab Fragment Goat Anti-Mouse IgG (H+L)
Reactivity
Mouse
Mouse
Applicable Processes
/
Conjugate Type
Alexa Fluor
Alexa Fluor
Scientific Score
6.08
Brand
Jackson ImmunoResearch Laboratories
Sizes
1 (0.75 mg)
Available From
3 Vendors
Price Range
$ 150.00 - $ 173.35
Catalog IDs
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Alexa Fluor® 647 AffiniPure Fab Fragment Donkey Anti-Rabbit IgG (H+L)
Reactivity
Rabbit
Rabbit
Applicable Processes
/
Conjugate Type
Alexa Fluor
Alexa Fluor
Scientific Score
4.39
Brand
Jackson ImmunoResearch Laboratories
Sizes
1 (0.75 mg)
Available From
2 Vendors
Price Range
$ 150.00 - $ 161.45
Catalog IDs
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Alexa Fluor® 647 AffiniPure Donkey Anti-Sheep IgG (H+L) SKU: 713-605-147
Reactivity
Sheep
Sheep
Applicable Processes
/
Conjugate Type
Alexa Fluor
Alexa Fluor
Scientific Score
4.39
Brand
Jackson ImmunoResearch Laboratories
Sizes
1 (0.5 mg)
Available From
2 Vendors
Price Range
$ 148.00 - $ 170.97
Catalog IDs
Added to comparison remove item
AffiniPure F(ab')2 Fragment Goat Anti-Mouse IgM, µ Chain Specific SKU: 115-006-075
Reactivity
Mouse
Mouse
Applicable Processes
/
Conjugate Type
/
Scientific Score
6.08
Brand
Jackson ImmunoResearch Laboratories
Sizes
1 (1 mg)
Available From
1 Vendor
Price
$ 157.00
Catalog IDs
Added to comparison remove item
Rabbit IgG (H&L) Antibody SKU: abx023914
Reactivity
Rabbit
Rabbit
Applicable Processes
/
Conjugate Type
/
Scientific Score
8.63
Brand
abbexa
Sizes
1 (1 mg)
Available From
1 Vendor
Price
$ 203.00
Catalog IDs
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Human IgG Antibody SKU: abx234077
Reactivity
Human
Human
Applicable Processes
ELISA, Western blot
ELISA, Western blot
Conjugate Type
/
Scientific Score
9.23
Brand
abbexa
Sizes
1 (100 µg)
Available From
1 Vendor
Price
$ 522.00
Catalog IDs
Added to comparison remove item
IgG (Fab) Antibody (PE) SKU: abx140309
Reactivity
Human
Human
Applicable Processes
ELISA, Flow cytometry, Western blot
ELISA, Flow cytometry, Western blot
Conjugate Type
PE (Phycoerythrin)
PE (Phycoerythrin)
Scientific Score
9.44
Brand
abbexa
Sizes
1 (0.1 mg)
Available From
1 Vendor
Price
$ 406.00
Catalog IDs
Added to comparison remove item
IgG (Fc) Antibody (FITC) SKU: abx140320
Reactivity
Human
Human
Applicable Processes
ELISA, Immunocytochemistry, Immunofluorescence, Western blot
ELISA, Immunocytochemistry, Immunofluorescence, Western blot
Conjugate Type
FITC (Fluorescein isothiocyanate)
FITC (Fluorescein isothiocyanate)
Scientific Score
9.44
Brand
abbexa
Sizes
1 (0.1 mg)
Available From
1 Vendor
Price
$ 348.00
Catalog IDs
Added to comparison remove item

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Secondary Antibody Function & Applications

Secondary antibodies bind to primary antibodies (on the Fc region) or fragments of antibodies (Fc or Fab), enabling indirect detection and purification of an antigen. They are essential for immunolabeling in ELISA, Western Blotting, Cellular Imaging and Flow Cytometry.

Targeting Primary Antibodies

These antibodies can target primary antibodies from a particular species, such as rat, known as anti-rat antibodies, allowing one secondary antibody to target multiple primary antibodies. On top of that, they can be specific to a particular class of antibodies such as IgG, or even IgG1.

Antigen Visualization

Some secondary antibodies can amplify the signal via conjugated fluorescent, colorimetric or chemiluminescent probes. Colorimetric assays detect alkaline phosphatase or horseradish peroxidase conjugates, and the streptavidin conjugate binding system enhances the colorimetric signal. Fluorescent assays detect Fluorescein (FITC), Rhodamine, TRITC, Cyanine (Cy3) or Phycoerythrin (R-PE).