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Rabbit IgG, FAntibody2 Full length Protein SKU: NBP1-97017
Applicable Processes
ELISA
ELISA
Reactivity
Rabbit
Rabbit
Conjugate Type
/
Scientific Score
5.52
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 235.87
Catalog IDs
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Rat IgG, FAntibody Full length Protein SKU: NBP1-97014
Applicable Processes
ELISA
ELISA
Reactivity
Rat
Rat
Conjugate Type
/
Scientific Score
5.52
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 235.87
Catalog IDs
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Goat IgG, FAntibody Full length Protein SKU: NBP1-97022
Applicable Processes
/
Reactivity
/
Conjugate Type
/
Scientific Score
1.90
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 235.87
Catalog IDs
Added to comparison remove item
IgG F(Antibody) SKU: NBP1-96824
Scientific Score
1.90
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 204.97
Catalog IDs
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IgG F(Antibody)2 SKU: NBP1-97097
Scientific Score
3.94
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 235.87
Catalog IDs
Added to comparison remove item
IgG F(Antibody)2 SKU: NBP1-97102
Scientific Score
1.90
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 235.87
Catalog IDs
Added to comparison remove item
IgG F(Antibody)2 SKU: NBP1-97187
Scientific Score
1.90
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 287.37
Catalog IDs
Added to comparison remove item
IgG F(Antibody)2 SKU: NBP1-97188
Scientific Score
1.90
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 287.37
Catalog IDs
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Mouse IgG, FAntibody2 Full length Protein SKU: NBP1-97018
Applicable Processes
ELISA
ELISA
Reactivity
Mouse
Mouse
Conjugate Type
/
Scientific Score
5.52
Brand
Novus Biologicals
Sizes
1 (1 Pack)
Available From
1 Vendor
Price
$ 235.87
Catalog IDs
Added to comparison remove item
Rat Anti-Mouse IgD-BIOT
Scientific Score
4.92
Brand
Southern Biotechnology
Sizes
1 (0.5 mg)
Available From
1 Vendor
Price
$ 238.87
Catalog IDs
Added to comparison remove item
FITC Goat Anti-Mouse IgG (H+L) SKU: AS001
Applicable Processes
Flow cytometry, Immunofluorescence
Flow cytometry, Immunofluorescence
Reactivity
/
Conjugate Type
/
Scientific Score
2.32
Brand
ABclonal
Sizes
1 (100 µl)
Available From
1 Vendor
Price
$ 28.00
Catalog IDs
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HRP Goat Anti-Human IgG (H+L) SKU: AS002
Applicable Processes
Immunohistochemistry, Western blot
Immunohistochemistry, Western blot
Reactivity
/
Conjugate Type
/
Scientific Score
2.32
Brand
ABclonal
Sizes
1 (100 µl)
Available From
1 Vendor
Price
$ 28.00
Catalog IDs
Added to comparison remove item

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Secondary Antibody Function & Applications

Secondary antibodies bind to primary antibodies (on the Fc region) or fragments of antibodies (Fc or Fab), enabling indirect detection and purification of an antigen. They are essential for immunolabeling in ELISA, Western Blotting, Cellular Imaging and Flow Cytometry.

Targeting Primary Antibodies

These antibodies can target primary antibodies from a particular species, such as rat, known as anti-rat antibodies, allowing one secondary antibody to target multiple primary antibodies. On top of that, they can be specific to a particular class of antibodies such as IgG, or even IgG1.

Antigen Visualization

Some secondary antibodies can amplify the signal via conjugated fluorescent, colorimetric or chemiluminescent probes. Colorimetric assays detect alkaline phosphatase or horseradish peroxidase conjugates, and the streptavidin conjugate binding system enhances the colorimetric signal. Fluorescent assays detect Fluorescein (FITC), Rhodamine, TRITC, Cyanine (Cy3) or Phycoerythrin (R-PE).