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Secondary Antibodies on ZAGENO

Goat Anti-Llama IgG:SureLight® APC SKU: 13393 Cayman Chemical
From $ 228.88 (1 Unit)
Sizes 1 (1 Unit)
Catalog IDs 13393-1
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Mouse Anti-6HIS IgG:SureLight® P3 SKU: 13449 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 13449-1
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Mouse Anti-c-myc IgG:DyLight® 488 SKU: 11247 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 11247-1
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Mouse Anti-c-myc IgG:SureLight® R-PE SKU: 13426 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 13426-1
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Mouse Anti-DYKDDDDK IgG:DyLight® 488 SKU: 11248 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 11248-1
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Mouse Anti-DYKDDDDK IgG:SureLight® PerCP SKU: 11275 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 11275-1
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Rabbit Anti-HA IgG:SureLight® PerCP SKU: 11277 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 11277-1
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Rabbit Anti-Mouse IgA Polyclonal FITC SKU: 10196 Cayman Chemical
From $ 96.82 (1 Unit)
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Catalog IDs 10196-1
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Rabbit Anti-S tag IgG:DyLight® 650 SKU: 11268 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 11268-1
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Rabbit Anti-V5 IgG:SureLight® R-PE SKU: 13430 Cayman Chemical
From $ 380.00 (1 Unit)
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Catalog IDs 13430-1
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Donkey Fab\'2 anti-Mouse IgG H+L Secondary Antibody SKU: NBP2-60648 Novus Biologicals
From $ 149.00 (1 mg)
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Catalog IDs NBP2-60648
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Donkey Fab\'2 anti-Rabbit IgG H+L Secondary Antibody SKU: NBP2-60650 Novus Biologicals
From $ 149.00 (1 mg)
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Catalog IDs NBP2-60650
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Secondary Antibody Function & Applications

Secondary antibodies bind to primary antibodies (on the Fc region) or fragments of antibodies (Fc or Fab), enabling indirect detection and purification of an antigen. They are essential for immunolabeling in ELISA, Western Blotting, Cellular Imaging and Flow Cytometry.

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Targeting Primary Antibodies

These antibodies can target primary antibodies from a particular species, such as rat, known as anti-rat antibodies, allowing one secondary antibody to target multiple primary antibodies. On top of that, they can be specific to a particular class of antibodies such as IgG, or even IgG1.

Antigen Visualization

Some secondary antibodies can amplify the signal via conjugated fluorescent, colorimetric or chemiluminescent probes. Colorimetric assays detect alkaline phosphatase or horseradish peroxidase conjugates, and the streptavidin conjugate binding system enhances the colorimetric signal. Fluorescent assays detect Fluorescein (FITC), Rhodamine, TRITC, Cyanine (Cy3) or Phycoerythrin (R-PE).