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Secondary Antibodies

Goat Anti-Mouse IgE:SureLight(R) R-PE SKU: 16646

Cayman Chemical

From
$ 228.88 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
16646-100
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Secondary Antibodies

Goat Anti-Mouse IgG Fc-Spec:SureLight(R) APC SKU: 16591

Cayman Chemical

From
$ 115.39 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
16591-100
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Secondary Antibodies

Goat Anti-Mouse IgG Fc-Spec:SureLight(R) R-PE SKU: 16649

Cayman Chemical

From
$ 115.39 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
16649-100
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Secondary Antibodies

Goat Anti-Mouse IgG:Alkaline Phosphatase SKU: 13459

Cayman Chemical

From
$ 172.03 (1 Unit)
Sizes
2 (1 - 100 Unit)
Catalog IDs
13459-1, 13459-100
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Secondary Antibodies

Goat F(Ab')2 Anti-Mouse IgG (H+L):SureLight(R) APC SKU: 16589

Cayman Chemical

From
$ 228.88 (100 µg)
Sizes
1 (100 µg)
Catalog IDs
16589-100
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Secondary Antibodies

Mouse Anti-c-myc IgG:Biotin SKU: 13469

Cayman Chemical

From
$ 380.00 (1 Unit)
Sizes
2 (1 - 100 Unit)
Catalog IDs
13469-1, 13469-100
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Secondary Antibodies

Mouse Anti-DYKDDDDK IgG:Alkaline Phosphatase SKU: 13464

Cayman Chemical

From
$ 380.00 (1 Unit)
Sizes
2 (1 - 100 Unit)
Catalog IDs
13464-1, 13464-100
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Secondary Antibodies

Cy™5 AffiniPure Donkey Anti-Human IgG (H+L)

Jackson ImmunoResearch Laboratories

Applicable Processes
Flow cytometry, Immunohistochemistry
Flow cytometry, Immunohistochemistry
Reactivity
Human
Human
Conjugate Type
/
/
From
$ 129.00 (0.5 mg)
Sizes
1 (0.5 mg)
Catalog IDs
709-175-149
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Secondary Antibodies

Amersham ECL Mouse IgG, HRP-linked F(ab')₂ fragment (from sheep)

Amersham

Applicable Processes
/
/
Reactivity
/
/
Conjugate Type
/
/
From
$ 716.85 (1 ml)
Sizes
1 (1 ml)
Catalog IDs
NA9310-1ML
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Secondary Antibodies

Amersham ECL Mouse IgG, HRP-linked whole Ab (from sheep) SKU: NXA931-1ML

Amersham

Applicable Processes
/
/
Reactivity
/
/
Conjugate Type
/
/
From
$ 310.76 (1 ml)
Sizes
1 (1 ml)
Catalog IDs
NXA931-1ML
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Secondary Antibodies

HRP Goat Anti-Rabbit IgG (H+L) SKU: AS014

ABclonal

Applicable Processes
ELISA, Western blot
ELISA, Western blot
Reactivity
/
/
Conjugate Type
/
/
From
$ 28.00 (100 µl)
Sizes
1 (100 µl)
Catalog IDs
AS014-100UL
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Secondary Antibodies

FITC Goat Anti-Rat IgG (H+L) SKU: AS019

ABclonal

Applicable Processes
Flow cytometry, Immunofluorescence
Flow cytometry, Immunofluorescence
Reactivity
/
/
Conjugate Type
/
/
From
$ 28.00 (100 µl)
Sizes
1 (100 µl)
Catalog IDs
AS019-100UL
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Secondary Antibody Function & Applications

Secondary antibodies bind to primary antibodies (on the Fc region) or fragments of antibodies (Fc or Fab), enabling indirect detection and purification of an antigen. They are essential for immunolabeling in ELISA, Western Blotting, Cellular Imaging and Flow Cytometry.

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Targeting Primary Antibodies

These antibodies can target primary antibodies from a particular species, such as rat, known as anti-rat antibodies, allowing one secondary antibody to target multiple primary antibodies. On top of that, they can be specific to a particular class of antibodies such as IgG, or even IgG1.

Antigen Visualization

Some secondary antibodies can amplify the signal via conjugated fluorescent, colorimetric or chemiluminescent probes. Colorimetric assays detect alkaline phosphatase or horseradish peroxidase conjugates, and the streptavidin conjugate binding system enhances the colorimetric signal. Fluorescent assays detect Fluorescein (FITC), Rhodamine, TRITC, Cyanine (Cy3) or Phycoerythrin (R-PE).