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Enzyme Assay Kits

Histone Deacetylase 8 (HDAC8) Inhibitor Screening Kit SKU : epi007

Sigma-Aldrich

Enzyme Name
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From
$ 507.00 (100 Essays)
Sizes
1 (100 Essays)
Catalog IDs
EPI007-1KT
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Enzyme Assay Kits

Adenosine Deaminase Activity Assay Kit (Fluorometric) SKU : epi020

Sigma-Aldrich

From
$ 295.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
EPI020-1KT
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Enzyme Assay Kits

β-Galactosidase Reporter Gene Activity Detection Kit SKU : gala

Sigma-Aldrich

Enzyme Name
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From
$ 213.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
GALA-1KT
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Enzyme Assay Kits

LookOut® Mycoplasma Elimination Kit SKU : MP0030

Sigma-Aldrich

Enzyme Name
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From
$ 217.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
MP0030-1KT
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Enzyme Assay Kits

Thioredoxin Reductase Assay Kit SKU : cs0170

Sigma-Aldrich

Enzyme Name
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From
$ 502.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
CS0170-1KT
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Enzyme Assay Kits

Glutathione-S-Transferase (GST) Assay Kit SKU : cs0410

Sigma-Aldrich

Enzyme Name
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From
$ 471.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
CS0410-1KT
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Enzyme Assay Kits

Chitinase Assay Kit SKU : cs0980

Sigma-Aldrich

Enzyme Name
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From
$ 602.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
CS0980-1KT
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Enzyme Assay Kits

Catalase Assay Kit SKU : cat100

Sigma-Aldrich

Enzyme Name
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From
$ 658.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
CAT100-1KT
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Enzyme Assay Kits

Caspase-6 Assay Kit, Fluorometric with Ac-VEID-AFC substrate SKU: 10077

Cepham Life Sciences

Enzyme Name
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From
$ 256.25 (50 Assays)
Sizes
2 (50 - 100 Assays)
Catalog IDs
10077, 10077-1
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Enzyme Assay Kits

Caspase-8 Assay Kit, Fluorometric with Ac-IETD-AFC substrate SKU: 10079

Cepham Life Sciences

Enzyme Name
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Detection Technique
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From
$ 256.25 (50 Assays)
Sizes
2 (50 - 100 Assays)
Catalog IDs
10079, 10079-1
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Enzyme Assay Kits

Caspase-10 Assay Kit, Fluorometric with Ac-AEVDD-AFC substrate SKU: 10083

Cepham Life Sciences

Enzyme Name
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Detection Technique
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Detection of
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From
$ 256.25 (50 Assays)
Sizes
2 (50 - 100 Assays)
Catalog IDs
10083, 10083-1
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Enzyme Assay Kits

β-Galactosidase Assay Kit SKU: 10435

Cepham Life Sciences

Enzyme Name
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From
$ 220.55 (400 Assays)
Sizes
1 (400 Assays)
Catalog IDs
10435
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Enzymatic Assays

Enzymes are specialized proteins or in some cases RNA molecules which speed up reactions in the cell. Without the presence of enzymes, chemical reactions would occur at a much slower pace - so much so life could not be sustained. Enzymes work by binding to the active site of their particular substrate, lower the reactions activation energy, thereby increasing the rate of reaction. This reaction rate is simply defined as the speed in which the reaction occurs, and is influenced by a number of factors such as temperature, pressure or concentration.

Given an enzyme’s ability to catalyze a reaction, they are vital for cellular processes such as signaling pathways, metabolism, and gene expression. Thus in knowing enzyme activity within the cell, i.e. the moles of substrate converted per unit time, scientists can infer the mechanism behind reactions. Enzyme assays are then standardized laboratory procedures which detect the level of enzymes present in a sample.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Detection Technique

So how are enzymes detected? By measuring the disappearance of the substrate, or the appearance of the product. This can be done in a variety of ways, but the most common are done either through colorimetry, fluorescence, or luminescence.

- Spectrometry measures the change of light absorption in the solution. Colorimetry is a type of spectrophotometric assay specific for measuring visible light.

- Fluorometric assays are similar to spectrophotometric assays in that they measure light. However, fluorescence measures light emittance after absorbing light at a different wavelength. So fluorometric assays measure the difference in fluorescence from the substrate to the product to measure the enzyme reaction.

- Luminescence measures the emission of light via chemical reaction. During the enzymatic reactions, light emittance can be used to infer product formation.

Enzyme Assays

Some standard enzyme assays measure the activity of kinase, phosphatase, superoxide dismutase, and Beta-Galactosidase.

Kinases are classed as phosphotransferases as they catalyze the transfer of phosphate groups from high-energy molecules to substrates. This process is known as phosphorylation (where a substrate gains a phosphate), and is a key process of glycolysis, the metabolic pathway common to most cells. Thus, kinases are essential for analyzing metabolic activity in cells.

Directly opposite of kinases are the phosphatases, which removes phosphate groups from a substrate.

Superoxide Dismutase catalyzes the partitioning of the superoxide ( O2- ) into ordinary 02 or H2O2. The superoxide molecule is a by-product of oxygen metabolism and can cause cell damage if not regulated. Thus superoxide dismutase is a major defensive mechanism within cells.

Beta-Galactosidase enzymes catalyze the breakdown of Beta-galactosides into monosaccharides, providing energy and free carbon to the cell.

Compare Enzyme Assay Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Video Credits

Video: Bozeman Science/Youtube