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Flow Cytometry

Score 8.25

LEGENDplex™ Human Bone Metabolism Panel 1 12-plex with Filter Plate

BioLegend

Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 1,400.00 (100 Tests)
Sizes
1 (100 Tests)
Catalog IDs
740655
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Flow Cytometry

Score 8.25

LEGENDplex™ Human Bone Metabolism Panel 1 12-plex with V-bottom Plate

BioLegend

Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 1,400.00 (100 Tests)
Sizes
1 (100 Tests)
Catalog IDs
740656
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Flow Cytometry

Score 8.25

LEGENDplex™ Human HSC Lymphoid Panel 9-plex with V-bottom Plate

BioLegend

Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 1,100.00 (100 Tests)
Sizes
1 (100 Tests)
Catalog IDs
740613
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Flow Cytometry

Score 8.28

LEGENDplex™ Human HSC Myeloid Panel 7-plex with V-bottom Plate

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Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 900.00 (100 Tests)
Sizes
1 (100 Tests)
Catalog IDs
740617
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Flow Cytometry

Score 3.55

LEGENDplex™ Human Immunoglobulin Isotyping Panel - IgE 1-plex with Filter Plate

BioLegend

Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 450.00 (100 Tests)
Sizes
1 (100 Tests)
Catalog IDs
740641
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Flow Cytometry

Score 3.56

LEGENDplex™ Human Immunoglobulin Isotyping Panel - IgGs 4-plex with Filter Plate

BioLegend

Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 600.00 (100 Tests)
Sizes
1 (100 Tests)
Catalog IDs
740714
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Flow Cytometry

Score 8.27

LEGENDplex™ Mouse Type 1/2 Interferon Panel 3-plex with V-bottom Plate

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Assay Type
/
/
Sample Type
/
/
Reactivity
Mouse
Mouse
From
$ 700.00 (100 Tests)
Sizes
1 (100 Tests)
Catalog IDs
740636
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Flow Cytometry

Score 3.56

LEGENDplex™ Human Hematopoietic Stem Cell Panel Standard

BioLegend

Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 50.00 (1 Vial)
Sizes
1 (1 Vial)
Catalog IDs
740619
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Flow Cytometry

Score 9.00

AcGFP Flow Cytometer Calibration Beads

TaKaRa

From
$ 282.00 (20 Assays)
Sizes
1 (20 Assays)
Catalog IDs
632594
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Flow Cytometry

Score 9.62

Actin (C-2)

Santa Cruz Biotechnology

Assay Type
/
/
Sample Type
/
/
Reactivity
Broad
Broad
From
$ 279.00 (1 Packs)
Sizes
1 (1 Packs)
Catalog IDs
sc-8432
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Flow Cytometry

Score 9.62

HCAM (DF1485)

Santa Cruz Biotechnology

Assay Type
/
/
Sample Type
/
/
Reactivity
Human, Mouse, Rat
Human, Mouse, Rat
From
$ 279.00 (1 Packs)
Sizes
1 (1 Packs)
Catalog IDs
sc-7297
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Flow Cytometry

Score 9.59

Histone H1 (AE-4)

Santa Cruz Biotechnology

Assay Type
/
/
Sample Type
/
/
Reactivity
Human
Human
From
$ 279.00 (1 Packs)
Sizes
1 (1 Packs)
Catalog IDs
sc-8030
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Flow Cytometry Applications

Flow Cytometry allows researchers to analyze multiple characteristics of single cells simultaneously, in the form of a fluid stream flowing through a beam of light. Properties that can be measured include size, number, internal complexity, and granularity. These measurements can be used to define different types of cell within a heterogeneous population as well as the purity of isolated subpopulations. These measurements can also indicate the level of apoptosis or proliferation within a population.

Fluorescence from labeled antibodies or ligands can also be detected, to quantify the expression of cell surface or intracellular molecules.

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Basic Staining Procedure

• Make a single-cell suspension for culture or tissue sample

• Incubate cells in tubes or microtiter plates

• Add unlabeled/fluorochrome-labeled antibodies

• Analyze on the flow cytometer

Cells can be sorted with the use of a specific type of flow cytometry known as FACS.

Measuring Size and Complexity

Sheath fluid forces the cell fluid through a nozzle which only allows one cell to pass through the light beam at a time. The cells create both forward scatter (used to measure size), and side scatter (used to measure internal complexity). These two readings can be used to identify the cell. For example, granulocytes create both a high forward scatter and side scatter, while lymphocytes produce low forward and side scatters.

Measuring Fluorescence

At the same time, the light beam will excite any fluorochromes/fluorophores present in the cell; these stains mark specific proteins (including antibodies) of interest. Sensors known as photomultiplier tubes (PMTs), within the flow cytometer, detect the light emitted from these fluorophores.

Each sensor will detect fluorescence at a specific wavelength (therefore only a certain few fluorophores) with the help of an appropriate filter. Short pass filters only allow wavelengths below a certain value, while long pass filters allow wavelengths above a certain wavelength.

Fluorescence Activated Cell Sorting (FACS)

FACS can separate live cells into subpopulations depending on their fluorescent labeling, i.e. which fluorophore has been used to stain them. The technology uses deflection plates to attract or repel cells based on the charge of the fluorophore.