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Flow Cytometry on ZAGENO

Brilliant Violet 510™ anti-mouse IgM Antibody BioLegend
Reagent Type Antibodies Applicable Processes Flow cytometry Quantity 50 µg
From $ 240.00 (50 µg)
Sizes 1 (50 µg)
Catalog IDs 406531
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Actin (C-2) Santa Cruz Biotechnology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow cytometry, ELISA Quantity /
From $ 279.00 (1 Packs)
Sizes 1 (1 Packs)
Catalog IDs sc-8432
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Histone H1 (AE-4) Santa Cruz Biotechnology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow cytometry, ELISA Quantity /
From $ 279.00 (1 Packs)
Sizes 1 (1 Packs)
Catalog IDs sc-8030
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Anti-PD-L1 antibody [28-8] Abcam
Reagent Type Antibodies Applicable Processes Blotting, Flow cytometry, Immunocytochemistry, Immunohistochemistry Quantity 50 µl
From $ 425.00 (50 µl)
Sizes 1 (50 µl)
Catalog IDs ab205921
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Rho A (26C4) Santa Cruz Biotechnology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow cytometry Quantity /
From $ 279.00 (1 Packs)
Sizes 1 (1 Packs)
Catalog IDs sc-418
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Phospho-Akt (Thr308) (D25E6) XP® Rabbit mAb Cell Signaling Technology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Immunofluorescence, Flow cytometry Quantity /
From $ 318.00 (100 µl)
Sizes 1 (100 µl)
Catalog IDs 13038S
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DYKDDDDK Tag (D6W5B) Rabbit mAb Cell Signaling Technology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Immunohistochemistry, Immunofluorescence, Flow cytometry, ChIP Quantity /
From $ 246.00 (100 µl)
Sizes 1 (100 µl)
Catalog IDs 14793S
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HCAM (DF1485) Santa Cruz Biotechnology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Immunofluorescence, Immunohistochemistry, Flow cytometry, ELISA Quantity /
From $ 279.00 (1 Packs)
Sizes 1 (1 Packs)
Catalog IDs sc-7297
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Phospho-Akt (Thr308) Antibody Cell Signaling Technology
Reagent Type Antibodies Applicable Processes Western blot, Immunoprecipitation, Flow cytometry Quantity /
From $ 287.00 (100 µl)
Sizes 2 (100 - 300 µl)
Catalog IDs 9275S, 9275L
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BAFF (human) monoclonal antibody (Buffy-1) (FITC conjugate) Enzo Life Sciences
Reagent Type Antibodies Applicable Processes Flow cytometry Quantity 100 Tests
From $ 399.00 (100 Tests)
Sizes 1 (100 Tests)
Catalog IDs ALX-804-128F-T100
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EdU Flow Cytometry Kit 555 sufficient for 50 assays Sigma-Aldrich
Reagent Type / Applicable Processes Flow cytometry Quantity 5 nmoles (PC) + 2 nmoles (NC)
From $ 667.00 (1 Kits)
Sizes 1 (1 Kits)
Catalog IDs BCK-FC555-50
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Stem Cell Control CD34+ Whole Blood Process Control BD
Reagent Type / Applicable Processes / Quantity /
From $ 263.00 (2 ml)
Sizes 1 (2 ml)
Catalog IDs 340991
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Flow Cytometry Applications

Flow Cytometry allows researchers to analyze multiple characteristics of single cells simultaneously, in the form of a fluid stream flowing through a beam of light. Properties that can be measured include size, number, internal complexity, and granularity. These measurements can be used to define different types of cell within a heterogeneous population as well as the purity of isolated subpopulations. These measurements can also indicate the level of apoptosis or proliferation within a population.

Fluorescence from labeled antibodies or ligands can also be detected, to quantify the expression of cell surface or intracellular molecules.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Basic Staining Procedure

• Make a single-cell suspension for culture or tissue sample

• Incubate cells in tubes or microtiter plates

• Add unlabeled/fluorochrome-labeled antibodies

• Analyze on the flow cytometer

Cells can be sorted with the use of a specific type of flow cytometry known as FACS.

Measuring Size and Complexity

Sheath fluid forces the cell fluid through a nozzle which only allows one cell to pass through the light beam at a time. The cells create both forward scatter (used to measure size), and side scatter (used to measure internal complexity). These two readings can be used to identify the cell. For example, granulocytes create both a high forward scatter and side scatter, while lymphocytes produce low forward and side scatters.

Measuring Fluorescence

At the same time, the light beam will excite any fluorochromes/fluorophores present in the cell; these stains mark specific proteins (including antibodies) of interest. Sensors known as photomultiplier tubes (PMTs), within the flow cytometer, detect the light emitted from these fluorophores.

Each sensor will detect fluorescence at a specific wavelength (therefore only a certain few fluorophores) with the help of an appropriate filter. Short pass filters only allow wavelengths below a certain value, while long pass filters allow wavelengths above a certain wavelength.

Fluorescence Activated Cell Sorting (FACS)

FACS can separate live cells into subpopulations depending on their fluorescent labeling, i.e. which fluorophore has been used to stain them. The technology uses deflection plates to attract or repel cells based on the charge of the fluorophore.