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QuickCOMP 2 Kit, 2 x 50 Tests
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Sample Type
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Reactivity
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Scientific Score
9.34
Brand
Beckman Coulter
Sizes
1 (100 Reactions)
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1 Supplier
Price
$ 445.00
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C6 Flow Cytometer 2-month Maintenance Kit
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Reactivity
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Scientific Score
2.32
Brand
BD
Sizes
1 (1 Kit)
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Price
$ 150.00
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Sera-Mag SpeedBeads Amine-Blocked Particles
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Sample Type
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Reactivity
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Scientific Score
8.71
Brand
GE Healthcare Life Sciences
Sizes
3 (1000 - 100000 µl)
Available From
3 Suppliers
Price Range
$ 168.12 - $ 2,278.40
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Sheath Fluid (30X)
Scientific Score
0.10
Brand
Tonbo Biosciences
Sizes
1 (20000000 µl)
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1 Supplier
Price
$ 29.00
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MACS GMP CD127-APC
Assay Type
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Sample Type
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Reactivity
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Scientific Score
9.08
Brand
Miltenyi Biotec
Sizes
1 (5000 µl)
Available From
1 Supplier
Price
$ 4,045.00
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MACS GMP CD4-PerCP
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Sample Type
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Reactivity
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Scientific Score
8.24
Brand
Miltenyi Biotec
Sizes
1 (5000 µl)
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1 Supplier
Price
$ 4,045.00
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Cyto-Chex BCT (5ml)
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Reactivity
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Scientific Score
0.10
Brand
Streck
Sizes
1 (100 Tubes)
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$ 1,224.05
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DNA Reagent Kit RUO GMP
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Sample Type
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Reactivity
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Scientific Score
9.32
Brand
BD
Sizes
1 (40 Reactions)
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Price
$ 278.00
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IMK-Lymphocyte IVD
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Sample Type
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Reactivity
Human
Human
Scientific Score
9.34
Brand
BD
Sizes
1 (50 Reactions)
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Price
$ 2,549.00
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IMK Plus IVD
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Sample Type
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Reactivity
Human
Human
Scientific Score
9.64
Brand
BD
Sizes
1 (50 Reactions)
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1 Supplier
Price
$ 2,090.00
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Regulatory T-Cell Function Kit RUO GMP
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Sample Type
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Reactivity
Human
Human
Scientific Score
9.79
Brand
BD
Sizes
1 (50 Reactions)
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Price
$ 1,295.00
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Lysing Solution 10X Concentrate IVD
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Scientific Score
8.29
Brand
BD
Sizes
1 (100000 µl)
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$ 348.00
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Flow Cytometry Applications

Flow Cytometry allows researchers to analyze multiple characteristics of single cells simultaneously, in the form of a fluid stream flowing through a beam of light. Properties that can be measured include size, number, internal complexity, and granularity. These measurements can be used to define different types of cell within a heterogeneous population as well as the purity of isolated subpopulations. These measurements can also indicate the level of apoptosis or proliferation within a population.

Fluorescence from labeled antibodies or ligands can also be detected, to quantify the expression of cell surface or intracellular molecules.

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Basic Staining Procedure

• Make a single-cell suspension for culture or tissue sample

• Incubate cells in tubes or microtiter plates

• Add unlabeled/fluorochrome-labeled antibodies

• Analyze on the flow cytometer

Cells can be sorted with the use of a specific type of flow cytometry known as FACS.

Measuring Size and Complexity

Sheath fluid forces the cell fluid through a nozzle which only allows one cell to pass through the light beam at a time. The cells create both forward scatter (used to measure size), and side scatter (used to measure internal complexity). These two readings can be used to identify the cell. For example, granulocytes create both a high forward scatter and side scatter, while lymphocytes produce low forward and side scatters.

Measuring Fluorescence

At the same time, the light beam will excite any fluorochromes/fluorophores present in the cell; these stains mark specific proteins (including antibodies) of interest. Sensors known as photomultiplier tubes (PMTs), within the flow cytometer, detect the light emitted from these fluorophores.

Each sensor will detect fluorescence at a specific wavelength (therefore only a certain few fluorophores) with the help of an appropriate filter. Short pass filters only allow wavelengths below a certain value, while long pass filters allow wavelengths above a certain wavelength.

Fluorescence Activated Cell Sorting (FACS)

FACS can separate live cells into subpopulations depending on their fluorescent labeling, i.e. which fluorophore has been used to stain them. The technology uses deflection plates to attract or repel cells based on the charge of the fluorophore.