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Immunoassays on ZAGENO

Panther Semi-Dry Electroblotters SKU : FP-2 Thermo Scientific
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Guinea Pig Clusterin/APOJ ELISA Kit Colorimetric SKU: NBP2-59986 Novus Biologicals
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Bovine Free Thyroxine ELISA Kit Colorimetric SKU: NBP2-60002 Novus Biologicals
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Goat Free Thyroxine ELISA Kit Colorimetric SKU: NBP2-60005 Novus Biologicals
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Equine Free Thyroxine ELISA Kit Colorimetric SKU: NBP2-60007 Novus Biologicals
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Equine Free Tri-Iodothyronine ELISA Kit Colorimetric SKU: NBP2-60019 Novus Biologicals
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Sheep Toxoplasmosis TOXO Antibody IgG ELISA Kit Colorimetric SKU: NBP2-60173 Novus Biologicals
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Human Albumin ELISA Kit Colorimetric SKU: NBP2-60461 Novus Biologicals
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Human Fibrinogen ELISA Kit Colorimetric SKU: NBP2-60466 Novus Biologicals
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Human Integrin alpha 2 beta/Integrin beta 3 ELISA Kit Colorimetric SKU: NBP2-60471 Novus Biologicals
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Human Kininogen ELISA Kit Colorimetric SKU: NBP2-60479 Novus Biologicals
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Monkey Albumin ELISA Kit Colorimetric SKU: NBP2-60482 Novus Biologicals
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Antibody-based Detection

Immunoassays use biochemistry to detect an analyte’s presence or concentration, whether it is a protein, antibody or small molecule. These assays rely upon highly-specific antibodies to detect antigens at low concentrations with high specificity, reducing the possibility of off-target binding.

Detection is possible in many different sample types such as blood, serum & plasma, urine or bodily fluids.


Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

ELISA

ELISA (Enzyme-Linked Immunosorbent Assay) kits contain plates coated or uncoated with capture/detection antibodies. Their detection format can also vary; competitive and sandwich are two examples. Complementary techniques include PCR, RNA preservation, and DNA sequencing.

Western Blotting

Western blotting allows detection of electrophoretically separated bands of protein via a specific primary antibody and a secondary antibody raised against the host of the primary antibody. The secondary antibody is commonly conjugated with a radiolabel, fluorophore or enzyme, such as horseradish peroxidase (HRP).

Immunoprecipitation

Immunoprecipitation utilizes antibodies attached to/able to attach to magnetic beads or agarose resin, which can then pull down proteins, RNA or chromatin (ChIP) out of solutions.