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ELISA Kits

Score 3.57

Mouse P-selectin (SELP) ELISA Kit SKU: abx570340

abbexa

ELISA Type
Sandwich
Sandwich
Target Molecule
P-selectin (SELP)
P-selectin (SELP)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 543.75 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx570340-96RXN
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ELISA Kits

Score 2.76

Rabbit C-C motif chemokine 2 (CCL2) ELISA Kit SKU: abx575494

abbexa

ELISA Type
/
/
Target Molecule
C-C motif chemokine 2 (CCL2)
C-C motif chemokine 2 (CCL2)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 558.25 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx575494-96RXN
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ELISA Kits

Score 2.76

Rabbit Interleukin 10 (IL10) ELISA Kit SKU: abx576165

abbexa

ELISA Type
/
/
Target Molecule
Interleukin 10 (IL10)
Interleukin 10 (IL10)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 572.75 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx576165-96RXN
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ELISA Kits

Score 2.76

Rabbit Interleukin 2 (IL2) ELISA Kit SKU: abx574432

abbexa

ELISA Type
/
/
Target Molecule
Interleukin 2 (IL2)
Interleukin 2 (IL2)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 572.75 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx574432-96RXN
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ELISA Kits

Score 3.38

Rabbit Metalloproteinase inhibitor 4 (TIMP4) ELISA Kit SKU: abx576363

abbexa

ELISA Type
/
/
Target Molecule
Metalloproteinase inhibitor 4 (TIMP4)
Metalloproteinase inhibitor 4 (TIMP4)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 717.75 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx576363-96RXN
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ELISA Kits

Score 2.76

Rabbit Myoglobin (MB) ELISA Kit SKU: abx576441

abbexa

ELISA Type
/
/
Target Molecule
Myoglobin (MB)
Myoglobin (MB)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 558.25 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx576441-96RXN
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ELISA Kits

Score 3.57

Rat AE Binding Protein 1 (AEBP1) ELISA Kit SKU: abx520684

abbexa

ELISA Type
Sandwich
Sandwich
Target Molecule
AE Binding Protein 1 (AEBP1)
AE Binding Protein 1 (AEBP1)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 558.25 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx520684-96RXN
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ELISA Kits

Score 3.38

Rat Clusterin (CLU) ELISA Kit SKU: abx573820

abbexa

ELISA Type
Sandwich
Sandwich
Target Molecule
Clusterin (CLU)
Clusterin (CLU)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 572.75 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx573820-96RXN
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ELISA Kits

Score 4.04

Rat Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1) ELISA Kit SKU: abx570103

abbexa

ELISA Type
Sandwich
Sandwich
Target Molecule
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1)
Nitric Oxide Synthase 1, Neuronal / nNOS (NOS1)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 572.75 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx570103-96RXN
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ELISA Kits

Score 2.76

Mouse Autoimmune Regulator (AIRE) ELISA Kit SKU: abx570926

abbexa

ELISA Type
/
/
Target Molecule
Autoimmune Regulator (AIRE)
Autoimmune Regulator (AIRE)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 638.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx570926-96RXN
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ELISA Kits

Score 4.88

Mouse Clusterin (CLU) ELISA Kit SKU: abx575060

abbexa

ELISA Type
Sandwich
Sandwich
Target Molecule
Clusterin (CLU)
Clusterin (CLU)
Sample Type
Biological fluids, Plasma, Serum
Biological fluids, Plasma, Serum
From
$ 572.75 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
abx575060-96RXN
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ELISA Kits

Score 4.83

ELISA Kit for Phosphatidylinositol (PI) SKU: CEG853Ge

Cloud-Clone

ELISA Type
/
/
Target Molecule
Phosphatidylinositol (PI)
Phosphatidylinositol (PI)
Sample Type
/
/
From
$ 567.00 (48 Reactions)
Sizes
4 (48 - 960 Reactions)
Catalog IDs
CEG853Ge-48RXN, CEG853Ge-96RXN, ...
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ELISA Functionality

ELISA (Enzyme-linked Immunosorbent Assay) detects particles with a simple color change by using antibodies. When antibodies are exposed to their corresponding antigen, it will bind to it to instigate an immune response. Researchers can utilize this ‘hook-like’ mechanism to detect particles like peptides, proteins, and hormones. There are also a variety of ELISA types, such as Direct or Indirect, Sandwich, and Competitive ELISA. Each of these have their own applications, allowing ELISA to be used for various tests, especially for medical reasons or toxicology reports.

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Experimental Setup

Simply put, what is required for an ELISA test, in general, are wells with which contain an antigen, detection enzymes, antibodies, and your sample. Although some types of ELISA work in different ways, the underlying principle always remain the same: the immobilization of an antigen. Modifications on this theme, like whether the antigen is caught with an antibody attached to a plate or suspended in solution, is what makes ELISA so useful for detection. Once particles of interest are stuck onto the ELISA plate, then all that is necessary is to wash the plate’s surface, separating attached from unattached particles.

To make researchers lives easier, detection enzymes (or any other tag) can be attached to primary or secondary antibodies, or proteins. Once a detection enzyme encounters its corresponding substrate, light is usually emitted and measured depending on your experimental setup (fluorometer, luminometer, spectrophotometer).

Our ELISA Troubleshoot provides further assistance.

Types of ELISA

Direct ELISA - Refers to direct detection; a primary antibody (labeled with a detection enzyme), reacts directly with an antigen, which is immobilized on the plate.

Indirect ELISA – Indirect detection; a labeled secondary antibody with known specificity for a primary antibody

Sandwich ELISA – one of the most common methods of ELISA, and the steps are as follows:

  • Capture antibody is placed onto a plate

  • Sample antigen is added, binding to the capture antibody

  • Detection antibody is introduced, binding also to the antigen

  • Secondary antibody with a detection enzyme is added, binding to the detection antibody

  • Substrate is added, thus triggering all successfully captured antigens within sample

Competitive ELISA

  • Sample antigen is incubated with complementary unlabeled

  • The antibody/antigen complexes are inserted into a well coated with competitive antigen.

  • Washing the plate removes unbound antibodies. (The higher the volume of antigen in the sample, the more Ag-Ab complexes present and therefore less unbound antibodies free to bind to the antigen coating the well)

  • Secondary antibody coupled to an enzyme is added, which binds the primary antibody.

  • Adding the substrate for the enzyme produces a chromogenic or fluorescent signal, depending on the reaction

  • Stopping the reaction prevents the signal from saturating

Enzyme-linked antigen can be used in place of enzyme-linked antibody. In this case labeled antigen competes for primary antibody binding sites with the sample antigen (unlabeled). A stronger signal is seen if the there is less antigen in the sample, as more labeled antigen is retained in the well.

Compare ELISA Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

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