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von Willebrand Factor (vWF) ELISA
ELISA Type
/
Target Molecule
von Willebrand Factor (vWF)
von Willebrand Factor (vWF)
Reactivity
Human
Human
Scientific Score
6.11
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 470.00 - $ 614.62
Catalog IDs
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TSH Ultrasensitive (Thyroid Stimulating Hormone) ELISA
ELISA Type
/
Target Molecule
TSH Ultrasensitive (Thyroid Stimulating Hormone)
TSH Ultrasensitive (Thyroid Stimulating Hormone)
Reactivity
Human
Human
Scientific Score
6.73
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 230.00 - $ 300.77
Catalog IDs
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Salivary Cortisol Ultrasensitive ELISA
ELISA Type
/
Target Molecule
Salivary Cortisol Ultrasensitive
Salivary Cortisol Ultrasensitive
Reactivity
Human
Human
Scientific Score
6.73
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 295.00 - $ 385.77
Catalog IDs
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URINARY CORTISOL ELISA
ELISA Type
/
Target Molecule
URINARY CORTISOL
URINARY CORTISOL
Reactivity
Human
Human
Scientific Score
6.73
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 230.00 - $ 300.77
Catalog IDs
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Urinary Isoprostane EIA Kit
ELISA Type
/
Target Molecule
Urinary Isoprostane EIA Kit
Urinary Isoprostane EIA Kit
Reactivity
Human
Human
Scientific Score
6.11
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 420.00 - $ 549.23
Catalog IDs
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Vitamin B12 ELISA
ELISA Type
/
Target Molecule
Vitamin B12
Vitamin B12
Reactivity
/
Scientific Score
4.44
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 680.00 - $ 889.23
Catalog IDs
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Swine TNF-α ELISA KIT
ELISA Type
/
Target Molecule
Swine TNF-α
Swine TNF-α
Reactivity
Pig
Pig
Scientific Score
6.11
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 390.00 - $ 510.00
Catalog IDs
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TBARS ELISA
ELISA Type
/
Target Molecule
TBARS
TBARS
Reactivity
Human
Human
Scientific Score
8.05
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 270.00 - $ 353.08
Catalog IDs
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TESTOSTERONE ELISA
ELISA Type
/
Target Molecule
TESTOSTERONE
TESTOSTERONE
Reactivity
Human
Human
Scientific Score
6.73
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 230.00 - $ 300.77
Catalog IDs
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TESTOSTERONE saliva ELISA
ELISA Type
/
Target Molecule
TESTOSTERONE saliva
TESTOSTERONE saliva
Reactivity
Human
Human
Scientific Score
6.73
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 230.00 - $ 300.77
Catalog IDs
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Human TGF-β1 ELISA KIT
ELISA Type
/
Target Molecule
TGF-β1
TGF-β1
Reactivity
Human
Human
Scientific Score
6.11
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 390.00 - $ 510.00
Catalog IDs
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Tg (Thyroglobulin) Ultrasensitive ELISA
ELISA Type
/
Target Molecule
Tg (Thyroglobulin) Ultrasensitive
Tg (Thyroglobulin) Ultrasensitive
Reactivity
Human
Human
Scientific Score
6.73
Brand
Eagle Biosciences
Sizes
1 (96 Wells)
Available From
2 Suppliers
Price Range
$ 570.00 - $ 745.38
Catalog IDs
Added to comparison remove item

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ELISA Functionality

ELISA (Enzyme-linked Immunosorbent Assay) detects particles with a simple color change by using antibodies. When antibodies are exposed to their corresponding antigen, it will bind to it to instigate an immune response. Researchers can utilize this ‘hook-like’ mechanism to detect particles like peptides, proteins, and hormones. There are also a variety of ELISA types, such as Direct or Indirect, Sandwich, and Competitive ELISA. Each of these have their own applications, allowing ELISA to be used for various tests, especially for medical reasons or toxicology reports.

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Experimental Setup

Simply put, what is required for an ELISA test, in general, are wells with which contain an antigen, detection enzymes, antibodies, and your sample. Although some types of ELISA work in different ways, the underlying principle always remain the same: the immobilization of an antigen. Modifications on this theme, like whether the antigen is caught with an antibody attached to a plate or suspended in solution, is what makes ELISA so useful for detection. Once particles of interest are stuck onto the ELISA plate, then all that is necessary is to wash the plate’s surface, separating attached from unattached particles.

To make researchers lives easier, detection enzymes (or any other tag) can be attached to primary or secondary antibodies, or proteins. Once a detection enzyme encounters its corresponding substrate, light is usually emitted and measured depending on your experimental setup (fluorometer, luminometer, spectrophotometer).

Our ELISA Troubleshoot provides further assistance.

Types of ELISA

Direct ELISA - Refers to direct detection; a primary antibody (labeled with a detection enzyme), reacts directly with an antigen, which is immobilized on the plate.

Indirect ELISA – Indirect detection; a labeled secondary antibody with known specificity for a primary antibody

Sandwich ELISA – one of the most common methods of ELISA, and the steps are as follows:

  • Capture antibody is placed onto a plate

  • Sample antigen is added, binding to the capture antibody

  • Detection antibody is introduced, binding also to the antigen

  • Secondary antibody with a detection enzyme is added, binding to the detection antibody

  • Substrate is added, thus triggering all successfully captured antigens within sample

Competitive ELISA

  • Sample antigen is incubated with complementary unlabeled

  • The antibody/antigen complexes are inserted into a well coated with competitive antigen.

  • Washing the plate removes unbound antibodies. (The higher the volume of antigen in the sample, the more Ag-Ab complexes present and therefore less unbound antibodies free to bind to the antigen coating the well)

  • Secondary antibody coupled to an enzyme is added, which binds the primary antibody.

  • Adding the substrate for the enzyme produces a chromogenic or fluorescent signal, depending on the reaction

  • Stopping the reaction prevents the signal from saturating

Enzyme-linked antigen can be used in place of enzyme-linked antibody. In this case labeled antigen competes for primary antibody binding sites with the sample antigen (unlabeled). A stronger signal is seen if the there is less antigen in the sample, as more labeled antigen is retained in the well.

Compare ELISA Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

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