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ELISA Kits

PathScan® Total Btk Sandwich ELISA Kit

Cell Signaling Technology

ELISA Type
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Target Molecule
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Sample Type
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From
$ 489.00 (96 Assays)
Sizes
1 (96 Assays)
Catalog IDs
24547C
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ELISA Kits

STOP Solution

Cell Signaling Technology

ELISA Type
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Target Molecule
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Sample Type
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From
$ 63.00 (50 ml)
Sizes
2 (50 - 250 ml)
Catalog IDs
7002P4, 7002P6
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ELISA Kits

Human Growth Hormone (hGH) SKU: EL10030

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
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From
$ 300.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10030-96RXN
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ELISA Kits

Interleukin-5 (IL-5) SKU: MEC1007

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
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/
From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
MEC1007-96RXN
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ELISA Kits

Tumor Necrosis Factor alpha (TNF-a) SKU: EL10019

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
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From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10019-96RXN
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ELISA Kits

Interleukin-6 (IL-6) SKU: EL10023

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
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From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10023-96RXN
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ELISA Kits

Interleukin-1 beta (IL-1β) SKU: EL10028

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
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From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10028-96RXN
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ELISA Kits

Interleukin-5 (IL-5) SKU: EL10035

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
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/
From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10035-96RXN
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ELISA Kits

Interleukin-1 alpha (IL-1α) SKU: EL10040

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
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From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10040-96RXN
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ELISA Kits

Interleukin-22 (IL-22) SKU: EL10055

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
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/
Sample Type
/
/
From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10055-96RXN
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ELISA Kits

Interleukin-18 (IL-18) SKU: EL10060

Anogen

ELISA Type
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/
Target Molecule
/
/
Sample Type
/
/
From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
EL10060-96RXN
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ELISA Kits

Interleukin-1β (IL-1β) SKU: MEC1010

Anogen

ELISA Type
Sandwich
Sandwich
Target Molecule
/
/
Sample Type
/
/
From
$ 450.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
MEC1010-96RXN
Added to comparison remove item

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ELISA Functionality

ELISA (Enzyme-linked Immunosorbent Assay) detects particles with a simple color change by using antibodies. When antibodies are exposed to their corresponding antigen, it will bind to it to instigate an immune response. Researchers can utilize this ‘hook-like’ mechanism to detect particles like peptides, proteins, and hormones. There are also a variety of ELISA types, such as Direct or Indirect, Sandwich, and Competitive ELISA. Each of these have their own applications, allowing ELISA to be used for various tests, especially for medical reasons or toxicology reports.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Experimental Setup

Simply put, what is required for an ELISA test, in general, are wells with which contain an antigen, detection enzymes, antibodies, and your sample. Although some types of ELISA work in different ways, the underlying principle always remain the same: the immobilization of an antigen. Modifications on this theme, like whether the antigen is caught with an antibody attached to a plate or suspended in solution, is what makes ELISA so useful for detection. Once particles of interest are stuck onto the ELISA plate, then all that is necessary is to wash the plate’s surface, separating attached from unattached particles.

To make researchers lives easier, detection enzymes (or any other tag) can be attached to primary or secondary antibodies, or proteins. Once a detection enzyme encounters its corresponding substrate, light is usually emitted and measured depending on your experimental setup (fluorometer, luminometer, spectrophotometer).

Our ELISA Troubleshoot provides further assistance.

Types of ELISA

Direct ELISA - Refers to direct detection; a primary antibody (labeled with a detection enzyme), reacts directly with an antigen, which is immobilized on the plate.

Indirect ELISA – Indirect detection; a labeled secondary antibody with known specificity for a primary antibody

Sandwich ELISA – one of the most common methods of ELISA, and the steps are as follows:

  • Capture antibody is placed onto a plate

  • Sample antigen is added, binding to the capture antibody

  • Detection antibody is introduced, binding also to the antigen

  • Secondary antibody with a detection enzyme is added, binding to the detection antibody

  • Substrate is added, thus triggering all successfully captured antigens within sample

Competitive ELISA

  • Sample antigen is incubated with complementary unlabeled

  • The antibody/antigen complexes are inserted into a well coated with competitive antigen.

  • Washing the plate removes unbound antibodies. (The higher the volume of antigen in the sample, the more Ag-Ab complexes present and therefore less unbound antibodies free to bind to the antigen coating the well)

  • Secondary antibody coupled to an enzyme is added, which binds the primary antibody.

  • Adding the substrate for the enzyme produces a chromogenic or fluorescent signal, depending on the reaction

  • Stopping the reaction prevents the signal from saturating

Enzyme-linked antigen can be used in place of enzyme-linked antibody. In this case labeled antigen competes for primary antibody binding sites with the sample antigen (unlabeled). A stronger signal is seen if the there is less antigen in the sample, as more labeled antigen is retained in the well.

Compare ELISA Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Video Credits

openmichigan/YouTube