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Human Mast cell tryptase ELISA Kit
ELISA Type
Competitive
Competitive
Target Molecule
/
Reactivity
/
Brand
MyBioSource
Sizes
4 (48 - 960 Reactions)
Available From
1 Vendor
Price Range
$ 440.00 - $ 5,415.00
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Protein A ELISA Kit (for Detection of Natural & Recombinant Protein)
ELISA Type
/
Target Molecule
/
Reactivity
/
Brand
Repligen
Sizes
1 (1 Kit)
Available From
1 Vendor
Price
$ 650.00
Catalog IDs
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HSD17B13 ELISA Kit (Human)
ELISA Type
/
Target Molecule
HSD17B13
HSD17B13
Reactivity
Human
Human
Brand
Aviva Systems Biology
Sizes
1 (1 Kit)
Available From
1 Vendor
Price
$ 549.00
Catalog IDs
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Prealbumin ELISA Kit (Mouse) (OKIA00111)
ELISA Type
/
Target Molecule
Prealbumin (OKIA00111)
Prealbumin (OKIA00111)
Reactivity
Mouse
Mouse
Brand
Aviva Systems Biology
Sizes
1 (1 Kit)
Available From
1 Vendor
Price
$ 489.00
Catalog IDs
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Hepatitis B Virus Surface Antibody ELISA Kit (Human)
ELISA Type
/
Target Molecule
Hepatitis B Virus Surface Antibody
Hepatitis B Virus Surface Antibody
Reactivity
Human
Human
Scientific Score
4.60
Brand
Aviva Systems Biology
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 455.00
Catalog IDs
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rT3 (Reverse Triiodothyronine) ELISA Kit SKU: E-EL-0120
ELISA Type
Sandwich
Sandwich
Target Molecule
rT3 (Reverse Triiodothyronine)
rT3 (Reverse Triiodothyronine)
Reactivity
Mouse
Mouse
Scientific Score
5.80
Brand
Elabscience
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 580.00
Catalog IDs
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Mouse VEGF-A (Vascular Endothelial Cell Growth Factor A) ELISA Kit SKU: E-EL-M1292
ELISA Type
Sandwich
Sandwich
Target Molecule
VEGF-A (Vascular Endothelial Cell Growth Factor A)
VEGF-A (Vascular Endothelial Cell Growth Factor A)
Reactivity
Mouse
Mouse
Scientific Score
4.60
Brand
Elabscience
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 650.00
Catalog IDs
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LTD4 (Leukotriene D4) ELISA Kit SKU: E-EL-0113
ELISA Type
Sandwich
Sandwich
Target Molecule
LTD4 (Leukotriene D4)
LTD4 (Leukotriene D4)
Reactivity
Mouse
Mouse
Scientific Score
5.79
Brand
Elabscience
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 650.00
Catalog IDs
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Human IgE (Immunoglobulin E) ELISA Kit SKU: E-EL-H2161
ELISA Type
Sandwich
Sandwich
Target Molecule
IgE (Immunoglobulin E)
IgE (Immunoglobulin E)
Reactivity
Human
Human
Scientific Score
4.29
Brand
Elabscience
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 650.00
Catalog IDs
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4-HNE (4-Hydroxynonenal) ELISA Kit SKU: E-EL-0128
ELISA Type
Sandwich
Sandwich
Target Molecule
4-HNE (4-Hydroxynonenal)
4-HNE (4-Hydroxynonenal)
Reactivity
Human
Human
Scientific Score
3.51
Brand
Elabscience
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 650.00
Catalog IDs
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A-CoA (Acetyl Coenzyme A) ELISA Kit SKU: E-EL-0125
ELISA Type
Sandwich
Sandwich
Target Molecule
A-CoA (Acetyl Coenzyme A)
A-CoA (Acetyl Coenzyme A)
Reactivity
Human
Human
Scientific Score
3.51
Brand
Elabscience
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 650.00
Catalog IDs
Added to comparison remove item
fT4 (Free Thyroxine) ELISA Kit SKU: E-EL-0122
ELISA Type
Sandwich
Sandwich
Target Molecule
fT4 (Free Thyroxine)
fT4 (Free Thyroxine)
Reactivity
Human
Human
Scientific Score
3.51
Brand
Elabscience
Sizes
1 (96 Reactions)
Available From
1 Vendor
Price
$ 650.00
Catalog IDs
Added to comparison remove item

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ELISA Functionality

ELISA (Enzyme-linked Immunosorbent Assay) detects particles with a simple color change by using antibodies. When antibodies are exposed to their corresponding antigen, it will bind to it to instigate an immune response. Researchers can utilize this ‘hook-like’ mechanism to detect particles like peptides, proteins, and hormones. There are also a variety of ELISA types, such as Direct or Indirect, Sandwich, and Competitive ELISA. Each of these have their own applications, allowing ELISA to be used for various tests, especially for medical reasons or toxicology reports.

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Experimental Setup

Simply put, what is required for an ELISA test, in general, are wells with which contain an antigen, detection enzymes, antibodies, and your sample. Although some types of ELISA work in different ways, the underlying principle always remain the same: the immobilization of an antigen. Modifications on this theme, like whether the antigen is caught with an antibody attached to a plate or suspended in solution, is what makes ELISA so useful for detection. Once particles of interest are stuck onto the ELISA plate, then all that is necessary is to wash the plate’s surface, separating attached from unattached particles.

To make researchers lives easier, detection enzymes (or any other tag) can be attached to primary or secondary antibodies, or proteins. Once a detection enzyme encounters its corresponding substrate, light is usually emitted and measured depending on your experimental setup (fluorometer, luminometer, spectrophotometer).

Our ELISA Troubleshoot provides further assistance.

Types of ELISA

Direct ELISA - Refers to direct detection; a primary antibody (labeled with a detection enzyme), reacts directly with an antigen, which is immobilized on the plate.

Indirect ELISA – Indirect detection; a labeled secondary antibody with known specificity for a primary antibody

Sandwich ELISA – one of the most common methods of ELISA, and the steps are as follows:

  • Capture antibody is placed onto a plate

  • Sample antigen is added, binding to the capture antibody

  • Detection antibody is introduced, binding also to the antigen

  • Secondary antibody with a detection enzyme is added, binding to the detection antibody

  • Substrate is added, thus triggering all successfully captured antigens within sample

Competitive ELISA

  • Sample antigen is incubated with complementary unlabeled

  • The antibody/antigen complexes are inserted into a well coated with competitive antigen.

  • Washing the plate removes unbound antibodies. (The higher the volume of antigen in the sample, the more Ag-Ab complexes present and therefore less unbound antibodies free to bind to the antigen coating the well)

  • Secondary antibody coupled to an enzyme is added, which binds the primary antibody.

  • Adding the substrate for the enzyme produces a chromogenic or fluorescent signal, depending on the reaction

  • Stopping the reaction prevents the signal from saturating

Enzyme-linked antigen can be used in place of enzyme-linked antibody. In this case labeled antigen competes for primary antibody binding sites with the sample antigen (unlabeled). A stronger signal is seen if the there is less antigen in the sample, as more labeled antigen is retained in the well.

Compare ELISA Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

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