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Immunoprecipitation Kits

Immunoprecipitation Starter Pack

GE Healthcare Life Sciences

Targets
/
/
Sample Amount
/
/
Technology
/
/
From
$ 561.00 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
17600235
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Immunoprecipitation Kits

Score 0.80

EZ-ChIP™

Merck

Targets
Genomic DNA
Genomic DNA
Sample Amount
/
/
Technology
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation
From
$ 477.00 (22 Reactions)
Sizes
1 (22 Reactions)
Catalog IDs
17-371
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Immunoprecipitation Kits

Score 8.30

Imprint® Chromatin Immunoprecipitation Kit

Sigma-Aldrich

Targets
Genomic DNA
Genomic DNA
Sample Amount
/
/
Technology
Chromatin Immunoprecipitation
Chromatin Immunoprecipitation
From
$ 291.00 (24 Reactions)
Sizes
2 (24 - 96 Reactions)
Catalog IDs
CHP1-24RXN, CHP1-96RXN
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Immunoprecipitation Kits

Classic Immunoprecipitation

G-Biosciences

Targets
Proteins
Proteins
Sample Amount
/
/
Technology
Spin column
Spin column
From
$ 268.00 (50 Reactions)
Sizes
1 (50 Reactions)
Catalog IDs
786-637
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Immunoprecipitation Kits

Score 3.30

ChIP-IT® Protein G Magnetic Beads

Active Motif

Targets
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/
Sample Amount
/
/
Technology
Protein G conjugated magnetic beads
Protein G conjugated magnetic beads
From
$ 250.00 (25 Reactions)
Sizes
1 (25 Reactions)
Catalog IDs
53014
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Immunoprecipitation Kits

Score 0.80

ChIP-IT® Express HT

Active Motif

Targets
/
/
Sample Amount
1-50 µg chromatin
1-50 µg chromatin
Technology
Protein G conjugated magnetic beads
Protein G conjugated magnetic beads
From
$ 1,050.00 (96 Reactions)
Sizes
1 (96 Reactions)
Catalog IDs
53018
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Immunoprecipitation Kits

EpiNext™ ChIP-Seq High-Sensitivity Kit

Epigentek

Targets
/
/
Sample Amount
1 x 10^5 to 1 x 10^6 cells, 5-50 mg tissue
1 x 10^5 to 1 x 10^6 cells, 5-50 mg tissue
Technology
ChIP-Seq
ChIP-Seq
From
$ 539.00 (12 Reactions)
Sizes
2 (12 - 24 Reactions)
Catalog IDs
P-2030-12, P-2030-24
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Immunoprecipitation Kits

MultiMACS Protein A Kit (4×96)

Miltenyi Biotec

From
$ 1,255.00 (384 Precipitations)
Sizes
1 (384 Precipitations)
Catalog IDs
130-092-945
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Immunoprecipitation Kits

Human Antibody Capture Kit, type 2

GE Healthcare Life Sciences

Targets
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/
Sample Amount
/
/
Technology
/
/
From
$ 884.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
29234600
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Immunoprecipitation Kits

EasySep™ Release Mouse Biotin Positive Selection Kit

STEMCELL Technologies

From
$ 689.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
17655
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Immunoprecipitation Kits

EasySep™ Mouse CD4+ T Cell Isolation Kit

STEMCELL Technologies

From
$ 607.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
19852
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Immunoprecipitation Kits

EasySep™ Mouse Monocyte Isolation Kit

STEMCELL Technologies

From
$ 668.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
19861
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IP Functionality

Immunoprecipitation, (such as ChIP), is a method of extracting a specific protein out of solution with a complementary antibody. That is, each antibody works as a specific ‘hook’ that captures its designated protein. When antibodies are attached to a substrate and introduced to the protein sample, those 'hooks' will catch and hold onto their specific protein, allowing the protein to be purified (via the substrate) from its original source; a key step in Sample Preparation.

Scientists use chromatin immunoprecipitation (ChIP) to determine the location of the DNA binding sites for a particular protein. In understanding DNA–protein interactions, scientists can answer questions about cell replication, gene expression, and the role molecular structure plays in cellular function.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Types of Immunoprecipitation

There are several types of immunoprecipitation, such as individual protein, RNA, or chromatin immunoprecipitation (ChIP); and different methods (direct or indirect) of performing these techniques, The direct method employs antibodies that are already attached to solid substrates, such as magnetic beads or agarose gels. The indirect method adds antibodies to a solution containing proteins, allows the solution to rest, then solid substrates like magnetic beads are added to bind to the antibodies. The indirect method is particularly useful for when protein concentration is low, or when the antibody affinity for proteins is weak. The direct method is the most commonly used in the lab.

Chromatin

One of the key uses for immunoprecipitation is studying the role chromatin plays in the cell. Chromatin is a molecular complex consisting of DNA, RNA, and protein; the structure of it, however, depends on the cellular phase. Nonetheless, chromatin serves to compact the genome into smaller segments to fit inside the cell, reinforce the DNA strand to allow mitosis, prevent damage to DNA, control gene expression, and aids in DNA replication.

Chromatin is formed via histones; small proteins which DNA wraps itself around to pack itself together. Chromatin can be lightly or tightly packed depending on whether the cell is in metaphase or interphase.

During interphase, the chromatin complex is loose; allowing DNA polymerase access to the DNA strand for DNA replication. Furthermore, genes which are actively transcribed are looser; genes that are not (i.e. turned off) are more tightly wound, preventing DNA polymerase access.

Chromatin Immunoprecipitation

As mentioned previously, to study chromatin scientists employ chromatin immunoprecipitation, commonly known as ChIP. ChIP is a means to extract chromatin from the cell, purify the DNA, and sequence it to determine where proteins, such as transcription factors, bind to DNA. In knowing the genetic sequence of where these proteins bind to DNA, scientists can better understand gene regulation and expression, as well as cellular replication.

ChIP is based on the principle that DNA binding proteins can be cross-linked to their binding site. So, following the principles of immunoprecipitation, proteins are crosslinked to their complementary DNA strand (via formaldehyde) in vivo. Cells are then lysed, and DNA is broken to smaller strands via sonification (with the proteins still attached). Then, immunoprecipitation is employed, purifying the chromatin complex.

To denature the DNA-protein crosslink, heat is applied (around 70 °C). Then, what is left are small DNA chunks which can then be amplified in PCR for further analysis. One tricky aspect, however, is designing the correct primers for the unknown DNA region you just extracted. One way around this is to clone the DNA strand into a plasmid vector and use the known primers for that vector to replicate your purified DNA.

Video and Image Credits

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