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Protein Quantification Kits

µQPRO - BCA Kit Micro

Cyanagen

Control Type
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/
Quantification Method
/
/
Sample Type
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/
From
$ 240.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
PRTD2,0500
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Protein Quantification Kits

Micro BCA Protein Assay Kit SKU: AR1110

Boster Bio

From
$ 80.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
AR1110-1KIT
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Protein Quantification Kits

Coomassie Plus Protein Assay Kit SKU: AR0145

Boster Bio

From
$ 20.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
AR0145-1KIT
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Protein Quantification Kits

In Vitro Bacterial Split GFP Fold ′n′ Glow™ Solubility Assay Kit (cyan) SKU : appa008

Sigma-Aldrich

Control Type
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Quantification Method
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/
Sample Type
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From
$ 611.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
APPA008-1KT
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Protein Quantification Kits

Easy II Protein Quantitative Kit (BCA)

TransGen Biotech

Control Type
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Quantification Method
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Sample Type
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From
$ 61.54 (100 ml)
Sizes
1 (100 ml)
Catalog IDs
DQ111-01
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Protein Quantification Kits

Focus™ SKU: 20840011

bioWORLD

From
$ 226.92 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
20840011-1
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Protein Quantification Kits

Micro Bicinchoninic Acid (BCA) Protein Assay SKU: 10479

Cepham Life Sciences

Control Type
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Quantification Method
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Sample Type
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From
$ 201.65 (500 Assays)
Sizes
1 (500 Assays)
Catalog IDs
10479
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Protein Quantification Kits

Score 8.10

Bradford Protein Assay Kit

OZ Biosciences

Control Type
Standard
Standard
Quantification Method
Bradford
Bradford
Sample Type
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From
$ 75.00 (1 Kit)
Sizes
3 (1 Kit)
Catalog IDs
BA00050, BA00070, ...
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Protein Quantification Kits

CB-X™ Protein Assay (With BSA Standard)

G-Biosciences

Control Type
Standard
Standard
Quantification Method
Bradford
Bradford
Sample Type
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/
From
$ 238.00 (500 Reactions)
Sizes
1 (500 Reactions)
Catalog IDs
786-12X
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Protein Quantification Kits

Score 8.10

Bicinchoninic Acid solution

Sigma-Aldrich

Control Type
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Quantification Method
BCA
BCA
Sample Type
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From
$ 313.00 (1000 ml)
Sizes
1 (1000 ml)
Catalog IDs
B9643-1L
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Protein Quantification Kits

BCA Protein Quantitation Kit

RayBiotech

Control Type
Standard
Standard
Quantification Method
BCA
BCA
Sample Type
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/
From
$ 195.00 (1000 Reactions)
Sizes
2 (1000 Assays)
Catalog IDs
68QT-BCAPro-S1000, K812-1000
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Protein Quantification Kits

Score 10.00

QuantiPro™ BCA Assay Kit

Sigma-Aldrich

Control Type
Standard
Standard
Quantification Method
BCA
BCA
Sample Type
/
/
From
$ 525.65 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
QPBCA-1KT
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Quantifying Protein in a Sample

Quantification of protein concentration is a necessary first step during any protein analysis. There are various detection methods to achieve this, ranging from simple UV absorbance measurements (as amino acids exhibit strong UV-light absorption), to HPLC. However, the more cost effective technique is through the use of simple colorimetric assays.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

The Bradford method

One technique to determine protein concentration and the amount of sample is the Bradford method. Its principle: negatively charged Coomassie® Blue dye binds to positively charged proteins. When no proteins are present in the sample, the dye absorbs at 465 nm and has a red color. However, when proteins are introduced, the color changes to blue and absorbs at 595 nm. This absorption can then be cross-referenced to a standard curve. However, Bradford assays are often inhibited by detergents and can be difficult to read at certain concentrations. Thus, the method for protein quantification used will have to be curtailed to your experimental needs.

Our Bradford Assay Troubleshoot will help solve any issues you encounter.

BCA Protein Assay

The BCA Protein Assay uses bicinchoninic acid (BCA) to colorimetrically detect the color change produced by the cuprous cation (Cu1+), which results from the reduction of Cu2+ to Cu1+ by protein in an alkaline medium.

In the biuret reaction, copper chelates with protein in an alkaline solution to form a light blue complex; peptides with three or more amino acid residues form a chelate complex with cupric ions in the presence of sodium potassium tartrate.

Two molecules of BCA then chelate with one reduced cuprous cation to form an intense purple-colored product. The BCA/copper complex is water-soluble; producing a strong absorbance at 562 nm, proportional to protein concentration. The BCA reagent is 100 times more sensitive than the original pale blue color.

The reaction that leads to BCA color formation is strongly influenced by four amino acid residues (cysteine, tyrosine, and tryptophan). However, the peptide backbone also contributes to color formation, minimizing variability caused by differences in protein composition.

Lowry Assay

The Lowry protein assay uses cupric sulfate and tartrate in an alkaline solution, which forms tetradentate copper protein complexes when protein is added. Folin-Ciocalteu Reagent is reduced proportionally to these chelated copper complexes, producing a blue water-soluble product which can be measured at 750nm.

Compare Bradford Assay Products!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison engine does not show the best kit, but instead helps you, the researcher, choose the most sutiable kit for your experiment.

Video Credits

Video: teachinhawaii/YouTube