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Enzymes on ZAGENO

Protease K IBI Scientific
From $ 137.71 (200 mg)
Sizes 1 (200 mg)
Catalog IDs IB05402
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Firepol (Thermostable TAQ) SKU: 11140006 bio-World
From $ 149.04 (1000 U)
Sizes 2 (1000 - 4000 U)
Catalog IDs 11140006-1, 11140006-2
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Taq DNA Polymerase (Recombinant) SKU: 22060707 bio-World
From $ 217.41 (1 U)
Sizes 1 (1 U)
Catalog IDs 22060707-1
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Endonuclease IV SKU: BEN0591 Bio Basic
From $ 85.58 (100 U)
Sizes 1 (100 U)
Catalog IDs BEN0591
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PFU DNA Polymerase SKU: B0093(D0090P) Bio Basic
From $ 40.51 (100 U)
Sizes 2 (100 - 500 U)
Catalog IDs B0093(D0090P)-100, B0093(D0090P)-500
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Recombinant Human Malic Enzyme 2 (ME2, His) SKU: RC512 Bio Basic
From $ 63.28 (5 µg)
Sizes 3 (5 - 1000 µg)
Catalog IDs RC512-15-5, RC512-15-10, ...
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T4 DNA Ligase SKU: B1125 Bio Basic
From $ 37.66 (200 U)
Sizes 1 (200 U)
Catalog IDs B1125
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T4 DNA Ligase with PEG SKU: B1442 Bio Basic
From $ 130.64 (1 kU)
Sizes 1 (1 kU)
Catalog IDs B1442
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Ubiquitin Conjugating Enzyme 7 (His6-tagged); Human UBC7 SKU: RC612 Bio Basic
From $ 63.28 (10 µg)
Sizes 3 (1 - 50 mg)
Catalog IDs RC612-18-1, RC612-18-10, ...
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Ubiquitin Conjugating Enzyme 9 (His6-tagged); Human UBC9 SKU: RC612 Bio Basic
From $ 63.28 (10 µg)
Sizes 3 (1 - 50 mg)
Catalog IDs RC612-20-1, RC612-20-10, ...
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Express Hi Fi DNA Polymerase SKU: EHF1101 Biomiga
From $ 354.38 (500 U)
Sizes 2 (500 - 2500 U)
Catalog IDs EHF1101-01, EHF1101-02
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Green Taq DNA Polymerase GenScript
From $ 60.00 (1000 Units)
Sizes 1 (1000 Units)
Catalog IDs E00043
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

The basics of enzymes

Enzymes lower the activation energy of a reaction, thus accelerating the reaction rate of the conversion of substrate to product. The increased rate can be by a factor of several million and without this increased reaction rate, life would not be able to be sustained here on Earth.
Most enzymes are proteins, but there are a few exceptions where RNA molecules can act as catalysts for reactions.
Since the enzyme is a catalyst, it is not consumed during the reaction. Thus, decreased reaction rates observed over time is not due to the enzyme concentration decreasing, but a combination of reduced availability of substrates and an accumulation of product.
Enzymes often work within very narrow pH and temperature ranges. Hence, it is essential that you use the correct buffers and optimal temperature ranges given in the protocol that is supplied with the enzyme.
Enzymes can lower the activation energy (ΔG‡, Gibbs free energy) of a reaction in several ways.

  • Stabilization of the transition state

  • Providing alternative pathways

  • Destabilization of the substrate ground state

The nomenclature of enzymes is determined by the substrate that is used in the reaction and with the addition of the suffix -ase. One example of this would be the alcohol-metabolizing enzyme alcohol dehydrogenase.
Generally, enzymes are divided into the following groups:

  • Oxidoreductases: catalyze oxidation/reduction reactions
  • Ligases: can join molecules with covalent bonds
  • Transferases: can transfer functional groups, such as polymerases
  • Isomerases: can catalyze isomerization changes
  • Lyases: cleave various bonds (not hydrolysis and oxidation)

Isozymes is the name given to enzymes that are different, but are catalyzing the same chemical reaction.

Our Restriction Enzyme Troubleshoot is useful for solving any potential problems.

Compare Enzymes

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the IPROOF™ HIGH-FIDELITY DNA POLYMERASE Kit from Bio-Rad accepts amplicons up to 37 kb.

  • that the TAQ PLUS DNA POLYMERASE Kit from G-Biosciences is the cheapest per unit.

  • that the PLATINUM™ SUPERFI™ GREEN DNA POLYMERASE Kit from Invitrogen is compatible with the widest range of templates.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.