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Reagents & Media on ZAGENO

RNaseOFF Ribonuclease Inhibitor Applied Biological Materials
Reagent Type Inhibitors Applicable Processes cDNA synthesis, In vitro transcription, Inhibition, Real-time PCR Quantity 4000 U
From $ 80.00 (100 µl)
Sizes 1 (100 µl)
Catalog IDs G138
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PBS 10X PH 7.4 1L Denville Scientific
From $ 99.30 (1 L)
Sizes 1 (1 L)
Catalog IDs 1005623
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Human Stellate cell media For Cell Growth Lonza
Reagent Type Growth media Applicable Processes Cultivation Quantity 250 ml
From $ 95.00 (250 ml)
Sizes 1 (250 ml)
Catalog IDs MCST250
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(S)-MG115 Cayman Chemical
Reagent Type Other reagents Applicable Processes / Quantity /
From $ 29.00 (1 mg)
Sizes 1 (1 mg)
Catalog IDs 15413
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Fluorescent Brightener 28 Sigma-Aldrich
Buffer Type / Concentration / Applicable Processes /
From $ 43.65 (1 g)
Sizes 1 (1 g)
Catalog IDs F3543-1G
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Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg/mL Solution in Water Invitrogen
Reagent Type Stains Applicable Processes / Quantity /
From $ 92.50 (10 ml)
Sizes 1 (10 ml)
Catalog IDs H3569
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Phusion HF Buffer New England Biolabs
Buffer Type / Concentration / Applicable Processes /
From $ 21.00 (6 ml)
Sizes 1 (6 ml)
Catalog IDs B0518S
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Q5 Reaction Buffer New England Biolabs
Buffer Type / Concentration / Applicable Processes /
From $ 25.00 (6 ml)
Sizes 1 (6 ml)
Catalog IDs B9027S
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Standard Taq Reaction Buffer I New England Biolabs
Buffer Type / Concentration / Applicable Processes /
From $ 21.00 (6 ml)
Sizes 1 (6 ml)
Catalog IDs B9014S
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Pierce™ 20X PBS Tween™ 20 Buffer Thermo Scientific
Buffer Type / Concentration / Applicable Processes /
From $ 85.00 (500 ml)
Sizes 1 (500 ml)
Catalog IDs 28352
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Corning® Matrigel® hESC-Qualified Matrix, *LDEV-Free Corning
Reagent Type Growth media Applicable Processes Cultivation Quantity 25 ml
From $ 1,014.55 (25 ml)
Sizes 1 (25 ml)
Catalog IDs 356277
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NucleoSpin® Plasmid EasyPure Macherey-Nagel
Buffer Type / Concentration / Applicable Processes /
From $ 43.00 (1 Pack)
Sizes 1 (1 Pack)
Catalog IDs 740953
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Reagent & Media Applications

Cellular and molecular experiments require a broad range of biochemical reagents for proper maintenance and analysis. Preparation for in vitro studies also necessitates culture media so that cells can proliferate to the required density and quantity necessary for an efficient and reliable investigation.

Cell culture media options vary in their constituents and are specifically manufactured to meet the demands of particular cell lines (e.g. mammalian) or techniques (e.g. transfections). Popular classes include DMEM, RPMI 1640, MEM, IMDM, Opti-MEM and DMEM/F-12.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Significant Reagents

Significant reagents:

• Channel Blockers – Impede ion channels present on cell membranes, useful for monitoring cell signaling. The most common are calcium and potassium channel blockers

• Chelators – e.g. EDTA, form a complex with metal ions; aid in preventing metal interference

• Detergents – solubilize hydrophobic molecules

• Stains – Help distinguish specific nucleic acids, proteins or organelles under the microscope, which would be otherwise difficult to visualize

• Buffers – Protect samples by resisting changes in pH. Also, block non-specific antibody binding and help track proteins during electrophoresis

• Inhibitors – Block or minimize target molecules from either; binding their complementary agonist, or carrying out a certain reaction. For example, they act as antagonists against enzymes; preventing binding of the substrate

• Drugs – Target a molecule or pathway to elicit a beneficial change

Lab Disinfectants remove contaminants to provide the clean or sterile conditions necessary for in vitro studies or delicate procedures such as RNA extraction; which requires an RNase-free environment. Common disinfectants include ethanol, oxidizing agents, and surfactants.