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Chelators on ZAGENO

Nitrilotriacetic acid SKU : 72559 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 59.60 (100 g)
Sizes 2 (100 - 500 g)
Catalog IDs 72559-100G, 72559-500G
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Tri-tert-butyl 1,4,7,10-tetraazacyclododecane-1,4,7-triacetate SKU : 75058 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 265.50 (100 mg)
Sizes 1 (100 mg)
Catalog IDs 75058-100MG-F
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(2-Hydroxypropyl)-γ-cyclodextrin SKU : 779113 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 426.50 (100 g)
Sizes 2 (100 - 500 g)
Catalog IDs 779113-100G, 779113-500G
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Methyl-β-cyclodextrin SKU : 779873 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 592.00 (100 g)
Sizes 5 (1 - 500 kg)
Catalog IDs 779873-1KG, 779873-10KG, ...
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1,7-Diaza-12-crown-4 SKU : 73753 Sigma-Aldrich
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From $ 146.50 (100 mg)
Sizes 1 (100 mg)
Catalog IDs 73753-100MG
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Edetate disodium United States Pharmacopeia (USP) Reference Standard Sigma-Aldrich
Reagent Type Chelators Applicable Processes / Quantity /
From $ 348.40 (200 mg)
Sizes 1 (200 mg)
Catalog IDs 1233009-200MG
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Ethylenediaminetetraacetic acid disodium salt dihydrate for electrophoresis, for molecular biology, 99.0-101.0% (titration) Sigma-Aldrich
Reagent Type Chelators Applicable Processes / Quantity /
From $ 29.50 (50 g)
Sizes 3 (50 - 500 g)
Catalog IDs E5134-50G, E5134-100G, ...
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Sodium citrate tribasic dihydrate SKU : 71409 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 136.00 (1 kg)
Sizes 1 (1 kg)
Catalog IDs 71409-1KG
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1,4,8,11-Tetraazacyclotetradecane-5,12-dione dihydrate SKU : 728322 Sigma-Aldrich
From $ 216.50 (250 mg)
Sizes 1 (250 mg)
Catalog IDs 728322-250MG
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Ferrous sulfate chelate solution SKU : f0518 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 31.20 (1 L)
Sizes 1 (1 L)
Catalog IDs F0518-1L
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Alfa Aesar™ Ethylene glycol-O,O'-bis(2-aminoethyl)-N,N,N',N'-tetraacetic acid, 0.5M aq. soln., pH 8.0 Alfa Aesar
Reagent Type / Applicable Processes / Quantity /
From $ 112.00 (100 ml)
Sizes 1 (100 ml)
Catalog IDs J60767AE
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Ethylenediaminetetraacetic acid dipotassium salt dihydrate SKU : 03659 Sigma-Aldrich
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From $ 84.40 (100 g)
Sizes 2 (100 - 500 g)
Catalog IDs 03659-100G, 03659-500G
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Chelator Function and Structure

Chelators or chelating agents are small water-soluble molecules which form complexes with metal ions and are occasionally fluorescent. As a complex (chelate) they are inert in solution; requiring high energy for dissociation. However, metal exchange is possible in labile complexes. Sequestering agents represent chelators which produce soluble metal complexes.

The functional groups of chelators are two of =O, -NH2 or -COO-. These are groups which donate an electron pair to the metal ion to form the complex and must be positioned correctly to allow ring formation. Chelators represent a major component of cellular metabolism.

Popular chelators include EDTA and Deferoxamine Mesylate.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Chelator Classifications

Natural Chelators (can aid in physiological systems transport iron and oxygen) include:

• Phosphates

• Ferrioxamines

• Phenolics

• Ionophores (monensin, valinomycin, gramicidin)

• Tetrapyrrols

Synthetic Chelators (suitable for separating metals in solutions) include:

• ionophores Nitrilotriacetic acid, NTA ortho-Phenanthroline

• 2,2'-Bipyridyl

• Salicylic acid

• Dimercaptopropanol

• Triethanolamine, TEA

• Ethylene glycol-bis-(2-aminoethyl)-N,N,N', N'-tetraacetic acid (EGTA)

• Ethylenediaminotetraacetic acid, EDTA Ethylenedioxy-diethylene-dinitrilo-tetraacetic acid

Chelator Applications

• Animal food addition to prevent precipitation or absorption of essential metals

• Elution in chelation affinity chromatography (EDTA or EGTA)

• Reduce metal interference when using metal indicators for measuring intracellular cation concentration (Ca2+ in FURA or QUIN).

• Reagent in quantitative complexometric determination of metals

• Removal of complex inhibitory heavy metals from buffer solutions

• Nutrient solution component for microorganisms/plants in hydroculture

Calcium Chelators

Calcium chelators have a high affinity for calcium ions through carboxylic groups. Binding can be affected by pH, other ions, and coordination to other proteins.

Calcium buffers are produced with the help of BAPTA. The chelator can also be used study calcium cell signaling by controlling cytosolic calcium concentration.

General calcium chelator use:

• Controlling Ca2+ levels intracellularly and extracellularly

• NMR analysis of Ca2+

• Research into calcium shuttling, mobilization and spatial calcium buffering