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Detergents on ZAGENO

Water Bath Treatment with 4 oz. Bottle and Solution Andwin Scientific
From $ 144.63 (32 oz)
Sizes 1 (32 oz)
Catalog IDs 190700
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DEOXY BIG CHAP (N,N'-Bis(3-D-gluconamidopropyl) deoxycholamide) SKU: 10244 Cepham Life Sciences
Applicable Processes /
From $ 221.60 (1 g)
Sizes 2 (1 - 2 g)
Catalog IDs 10244, 10244-1
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Sulfobetaine 3-10 (SB 3-10) (n-Decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate) SKU: 10329 Cepham Life Sciences
Applicable Processes /
From $ 38.90 (1 g)
Sizes 2 (1 - 5 g)
Catalog IDs 10329, 10329-1
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Aqueous dispersant for multi-walled carbon nanotubes Alfa Aesar
From $ 58.38 (10 ml)
Sizes 1 (10 ml)
Catalog IDs 44276-AB
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TWEEN® 80 SKU : P4780 Sigma-Aldrich
From $ 25.08 (100 ml)
Sizes 3 (1 - 500 gal)
Catalog IDs P4780-1GA, P4780-100ML, ...
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Sodium n-dodecyl sulfate, ultrapure, 99% Alfa Aesar
Applicable Processes /
From $ 71.08 (25 g)
Sizes 3 (25 - 500 g)
Catalog IDs J64241-14, J64241-22, ...
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Polyoxyethylenesorbitan monolaurate Tocris
Applicable Processes /
From $ 49.00 (500 ml)
Sizes 1 (500 ml)
Catalog IDs 3165/500ML
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Terg-a-zyme® enzyme detergent Sigma-Aldrich
Applicable Processes /
From $ 42.30 (1 EA)
Sizes 1 (1 EA)
Catalog IDs Z273287-1EA
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Benzalkonium chloride SKU : b6295 Sigma-Aldrich
Applicable Processes /
From $ 72.00 (100 g)
Sizes 2 (100 - 500 g)
Catalog IDs B6295-100G, B6295-500G
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C7BzO SKU : c0856 Sigma-Aldrich
From $ 115.00 (1 g)
Sizes 1 (1 g)
Catalog IDs C0856-1G
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CHAPSO SKU : c3649 Sigma-Aldrich
Applicable Processes /
From $ 87.00 (500 mg)
Sizes 4 (1 - 500 g)
Catalog IDs C3649-1G, C3649-5G, ...
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CHAPSO SKU : c4695 Sigma-Aldrich
Applicable Processes /
From $ 153.00 (500 mg)
Sizes 3 (1 - 500 g)
Catalog IDs C4695-1G, C4695-5G, ...
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Detergent Function & Application

Detergents effectively solubilize hydrophobic molecules; they are small amphipathic molecules consisting of a long alkyl chain (hydrophobic) tail attached to hydrophilic/ water solubility enhancing functional groups (a polar head). Examples include sodium dodecyl sulfate, CHAPS, and polyethylene glycol.

Applications of detergents include liposome preparation, cell lysis, analyte precipitation in solutions, solubilization of proteins and lipids, DNA & RNA extraction, as well as preventing non-specific binding in immunoassays and westerns.

The detergent type depends on the charge of the head group, whether it is ionic, non-ionic or zwitterionic.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Anionic Detergents

Anionic Detergents have a negative charge and are used in DNA or RNA purification, due to their ability to disrupt cell membranes and denature proteins. However, these detergents are sensitive to ionic strength, pH and counterion nature. They may also interfere with charge-based analytical methods. Examples include:

• Sodium cholate

• Sodium deoxycholate

• Sodium glycocholate

• Sodium taurocholate

• Sodium Taurodeoxycholate

• N-lauryl sarcosine (Sarkosyl solution)

• Lithium dodecyl sulfate (LDS)

• Sodium dodecyl sulfate (SDS) (a vital component of SDS-PAGE)

Cationic Detergents

Cationic Detergents have a positive charge and are therefore useful at residual anionic detergents. They can be used as surfactants in drug or vaccine delivery, while also applying to DNA extraction. Due to being characteristically germicidal, they may also be used as disinfectants. Examples include CTAB (hexadecyltrimethylammonium bromide) and TTAB (Trimethyltetradecyl ammonium bromide).

Non-ionic Detergents

Non-ionic detergents are milder and therefore less likely to denature proteins, however shorter hydrophobic chain lengths may cause some denaturation.

• Poly (oxyethylene) ethers (& related polymers) have hydrophobic tails and a neutral, polar head group. Popular oxyethylene polymers are Tween 20 and Tween 80

• Bile Salts have a steroid core with a polar or apolar structure; they are less denaturing than similar detergents

• Glycosidic Detergents have a carbohydrate (glucose/maltose) polar head group and a nonpolar tail of between 7-14 carbons.

Zwitterionic Detergents

Zwitterionic detergents have a neutral charge and are therefore also less denaturing; critical applications are disrupting protein-protein bonds and reducing aggregation. These assets are put to good use in mass spectrometry, chromatography, electrophoresis and solubilization of cellular organelles and inclusion bodies.