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Stains on ZAGENO

Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg/mL Solution in Water Invitrogen
Reagent Type Stains Applicable Processes / Quantity /
From $ 92.50 (10 ml)
Sizes 1 (10 ml)
Catalog IDs H3569
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Eriochrome® Black T Sigma-Aldrich
Reagent Type Stains Applicable Processes Quantification Quantity 100 g, 500 g, 1000 g
From $ 23.80 (100 g)
Sizes 3 (100 - 1000 g)
Catalog IDs 858390-100G, 858390-500G, ...
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Sulforhodamine B, 97%, ACROS Organics™ Fisher Scientific
Reagent Type Stains Applicable Processes Molecular labeling Quantity 1 g
From $ 32.65 (1 g)
Sizes 1 (1 g)
Catalog IDs AC333130010
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Sulforhodamine B sodium salt Sigma-Aldrich
Reagent Type Stains Applicable Processes Cultivation Quantity 1 g
From $ 28.40 (1 g)
Sizes 1 (1 g)
Catalog IDs S1402-1G
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PKH26 Red Fluorescent Cell Linker Kit for General Cell Membrane Labeling Sigma-Aldrich
Reagent Type Stains Applicable Processes Molecular labeling Quantity 500 µl
From $ 730.00 (500 µl)
Sizes 1 (500 µl)
Catalog IDs PKH26GL-1KT
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Stain Buffer (BSA) BD Pharmingen
Reagent Type / Applicable Processes / Quantity /
From $ 95.00 (500 ml)
Sizes 1 (500 ml)
Catalog IDs 554657
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LIVE/DEAD™ Fixable Green Dead Cell Stain Kit, for 488 nm excitation Invitrogen
Reagent Type / Applicable Processes / Quantity /
From $ 269.00 (200 Assays)
Sizes 1 (200 Assays)
Catalog IDs L23101
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ArC™ Amine Reactive Compensation Bead Kit (for use with LIVE/DEAD™ Fixable dead cell stain kits) Invitrogen
Reagent Type / Applicable Processes / Quantity /
From $ 311.00 (1 Kits)
Sizes 1 (1 Kits)
Catalog IDs A10346
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Blue Dextran Loading Dye SKU : 10362 Cepham Life Sciences
Reagent Type / Applicable Processes / Quantity /
From $ 22.00 (1.5 ml)
Sizes 2 (1.5 - 6 ml)
Catalog IDs 10362, 10362-1
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NeuroFluor™ NeuO STEMCELL Technologies
Reagent Type / Applicable Processes / Quantity /
From $ 329.00 (100 µl)
Sizes 1 (100 µl)
Catalog IDs 01801
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5(6) - FAM AnaSpec
Reagent Type Stains Applicable Processes Molecular labeling Quantity 250 mg, 1000 mg, 10000 mg, 25000 mg
From $ 66.00 (250 mg)
Sizes 4 (250 - 25000 mg)
Catalog IDs AS-81002, AS-81002-1, ...
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Oil Red O Sigma-Aldrich
Reagent Type Stains Applicable Processes Molecular labeling Quantity 25 g
From $ 24.30 (25 g)
Sizes 1 (25 g)
Catalog IDs O0625-25G
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Staining

Staining is a technique used to identify target molecules and provide contrast in an image, usually in microscopy. For example, biological tissues ranging from muscle fibers to organelles, are often stained to highlight particular regions for viewing with a microscope. Overall, staining involves adding a class specific dye, such as DNA, proteins, lipids or carbohydrates, to quantify and qualify their presence.

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Staining Methods

There are two common types of staining in biochemistry:

  • In vivo staining involves dyeing living tissue by causing cells or structures to take up the stain’s color. In doing so reveals
  • In vitro staining is done outside of the biological context. In vitro staining is usually used with other methods such as fixation and sample preparation. For example, the crystal violet stain only works with Gram-positive bacteria. If then, another stain is used that works on all cell-types, then Gram-negative bacteria can be identified.

In Vitro Methods

In vitro methods vary given the biological materials at hand, but in general, they follow these steps:

Preparation, either through

  • Fixation: preserve the shape of cells or tissues
  • Permeabilization: applying a mild surfactant to dissolve cell membranes, allowing for greater access to organelles
  • Mounting: applying a sample to a glass microscope slide.

Staining

  • Immerse target sample in the stain solution, and rinsing to remove excess dye.
  • If this is ineffective, then a mordant is usually required; a chemical compound which reacts with the stain to produce a colored precipitate.

Some common examples of staining techniques are:

  • Gram Staining: Determines gram status to classify bacteria, which are either Gram-negative or Gram-positive. Based on cell wall composition, Gram-positive bacteria stain a dark blue to violet, whereas Gram-negative appear red or pink.
  • Haematoxylin and eosin staining: Used in histology to examine thin sections of tissues. Hematoxylin targets cell nuclei and stains them blue, whereas eosin stains the cytoplasm pink.
  • Masson’s Trichrome: a three-color staining method used to distinguish cells from connective tissue.

Types of Stains:

  • Acridine Orange: a fluorescent cationic dye for cell cycle determination targeting nucleic acids
  • Coomassie blue: a stain used in gel electrophoresis that stains proteins blue
  • Crystal Violet: stains cell walls purple, and is the primary stain used in Gram staining
  • DAPI: binds to DNA, and shines a blue fluorescence when viewed under ultraviolet light
  • Eosin: a counterstain to hematoxylin and to color cytoplasmic material
  • Ethidium Bromide: colors DNA a fluorescent red-orange color.
  • Hematoxylin: targets the nucleus, and is the counter part to eosin
  • Iodine: indicates the presence of starch
  • Methylene blue: stains the nucleus of animal cells