Experience more EUREKA moments with ZAGENO
Call Free Hotline - 617 855 5944 Mo-Fr, 9am-5pm
ZAGENO
Results: 1-12 of 100+ products
Sort by:
Show as:

Stains on ZAGENO

Hoechst 33258, Pentahydrate (bis-Benzimide) - 10 mg/mL Solution in Water Invitrogen
Reagent Type Stains Applicable Processes / Quantity /
From $ 92.50 (10 ml)
Sizes 1 (10 ml)
Catalog IDs H3569
Added to comparison view - remove
View Product
Atto MB2 SKU : 75118 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 104.40 (1 mg)
Sizes 1 (1 mg)
Catalog IDs 75118-1MG
Added to comparison view - remove
View Product
Gram’s decolorizer solution SKU : 75482 Sigma-Aldrich
From $ 22.10 (250 ml)
Sizes 2 (2.5 - 250 L)
Catalog IDs 75482-2.5L-F, 75482-250ML-F
Added to comparison view - remove
View Product
Atto Rho13 Biotin SKU : 76141 Sigma-Aldrich
From $ 248.00 (1 mg)
Sizes 1 (1 mg)
Catalog IDs 76141-1MG
Added to comparison view - remove
View Product
cis-Bis(isothiocyanato)(2,2′-bipyridyl-4,4′-dicarboxylato)(4,4′-di-nonyl-2′-bipyridyl)ruthenium(II) SKU : 703168 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 216.00 (1 g)
Sizes 1 (1 g)
Catalog IDs 703168-1G
Added to comparison view - remove
View Product
cis-Bis(isothiocyanato)bis(2,2′-bipyridyl-4,4′-dicarboxylato)ruthenium(II) SKU : 703206 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 238.50 (1 g)
Sizes 1 (1 g)
Catalog IDs 703206-1G
Added to comparison view - remove
View Product
Phenanthridine SKU : 77490 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 38.70 (1 g)
Sizes 1 (1 g)
Catalog IDs 77490-1G
Added to comparison view - remove
View Product
Atto 520 NHS ester SKU : 77810 Sigma-Aldrich
From $ 73.90 (1 mg)
Sizes 1 (1 mg)
Catalog IDs 77810-1MG-F
Added to comparison view - remove
View Product
Atto 490LS maleimide SKU : 78363 Sigma-Aldrich
From $ 218.30 (1 mg)
Sizes 1 (1 mg)
Catalog IDs 78363-1MG
Added to comparison view - remove
View Product
Atto 550 PPE SKU : 78998 Sigma-Aldrich
From $ 442.10 (1 mg)
Sizes 1 (1 mg)
Catalog IDs 78998-1MG
Added to comparison view - remove
View Product
3-Phenylumbelliferone SKU : 79279 Sigma-Aldrich
Reagent Type / Applicable Processes / Quantity /
From $ 558.00 (500 mg)
Sizes 1 (500 mg)
Catalog IDs 79279-500MG
Added to comparison view - remove
View Product
Eriochrome® Black T Sigma-Aldrich
Reagent Type Stains Applicable Processes Quantification Quantity 100 g, 500 g, 1000 g
From $ 23.80 (100 g)
Sizes 3 (100 - 1000 g)
Catalog IDs 858390-100G, 858390-500G, ...
Added to comparison view - remove
View Product

Staining

Staining is a technique used to identify target molecules and provide contrast in an image, usually in microscopy. For example, biological tissues ranging from muscle fibers to organelles, are often stained to highlight particular regions for viewing with a microscope. Overall, staining involves adding a class specific dye, such as DNA, proteins, lipids or carbohydrates, to quantify and qualify their presence.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Staining Methods

There are two common types of staining in biochemistry:

  • In vivo staining involves dyeing living tissue by causing cells or structures to take up the stain’s color. In doing so reveals
  • In vitro staining is done outside of the biological context. In vitro staining is usually used with other methods such as fixation and sample preparation. For example, the crystal violet stain only works with Gram-positive bacteria. If then, another stain is used that works on all cell-types, then Gram-negative bacteria can be identified.

In Vitro Methods

In vitro methods vary given the biological materials at hand, but in general, they follow these steps:

Preparation, either through

  • Fixation: preserve the shape of cells or tissues
  • Permeabilization: applying a mild surfactant to dissolve cell membranes, allowing for greater access to organelles
  • Mounting: applying a sample to a glass microscope slide.

Staining

  • Immerse target sample in the stain solution, and rinsing to remove excess dye.
  • If this is ineffective, then a mordant is usually required; a chemical compound which reacts with the stain to produce a colored precipitate.

Some common examples of staining techniques are:

  • Gram Staining: Determines gram status to classify bacteria, which are either Gram-negative or Gram-positive. Based on cell wall composition, Gram-positive bacteria stain a dark blue to violet, whereas Gram-negative appear red or pink.
  • Haematoxylin and eosin staining: Used in histology to examine thin sections of tissues. Hematoxylin targets cell nuclei and stains them blue, whereas eosin stains the cytoplasm pink.
  • Masson’s Trichrome: a three-color staining method used to distinguish cells from connective tissue.

Types of Stains:

  • Acridine Orange: a fluorescent cationic dye for cell cycle determination targeting nucleic acids
  • Coomassie blue: a stain used in gel electrophoresis that stains proteins blue
  • Crystal Violet: stains cell walls purple, and is the primary stain used in Gram staining
  • DAPI: binds to DNA, and shines a blue fluorescence when viewed under ultraviolet light
  • Eosin: a counterstain to hematoxylin and to color cytoplasmic material
  • Ethidium Bromide: colors DNA a fluorescent red-orange color.
  • Hematoxylin: targets the nucleus, and is the counter part to eosin
  • Iodine: indicates the presence of starch
  • Methylene blue: stains the nucleus of animal cells