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PCR (Polymerase Chain Reaction) on Zageno

TransforMax™ EC100D™ pir+ Electrocompetent E.coli Epicentre
Competence Electro Antibiotic Resistance Streptomycin Bacterial Strain /
From $ 220.00 (500 µl)
Sizes 1 (500 µl)
Catalog IDs ECP09500
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2X One-Step RT-PCR Master Mix Norgen Biotek
Templates Total RNA Fidelity No proofreading activity Amplicon Size < 1.5 kb
From $ 243.00 (100 Reactions)
Sizes 3 (100 - 500 Reactions)
Catalog IDs 28113, 28114, 28115
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MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit Epicentre
Templates Cells Template Amount 1-1000 cells Time to Sample < 9h 30 min
From $ 220.00 (10 Reactions)
Sizes 1 (10 Reactions)
Catalog IDs MBCL90310
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MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit Epicentre
Templates Total RNA, mRNA Template Amount < 1 µg total RNA Time to Sample > 70 min
From $ 216.00 (50 Reactions)
Sizes 1 (50 Reactions)
Catalog IDs MM070150
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TransforMax™ EPI300™ Chemically Competent E. coli Epicentre
Competence Chemical Antibiotic Resistance Streptomycin Bacterial Strain /
From $ 194.00 (10 Reactions)
Sizes 1 (10 Reactions)
Catalog IDs C300C105
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Tth Polymerase Bioron
Templates Total RNA Fidelity No proofreading activity Amplicon Size > 2-3 kb
From $ 126.00 (500 Units)
Sizes 2 (500 - 2500 Units)
Catalog IDs 104005, 104025
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Taq Master Mix Bioron
Templates Genomic DNA Fidelity No proofreading activity Amplicon Size > 5 kb
From $ 55.00 (200 Reactions)
Sizes 2 (200 - 1000 Reactions)
Catalog IDs 101605, 101625
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GreenHot Master Mix Bioron
Fluorescent Dye Included Templates cDNA Amplicon Size /
From $ 96.00 (200 Reactions)
Sizes 2 (200 - 1000 Reactions)
Catalog IDs 119205, 119225
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SafeView™ Classic Applied Biological Materials
Reagent Type Staining solutions Applicable Processes End-point PCR, Real-time PCR Quantity 1000 µl
From $ 30.00 (1000 µl)
Sizes 1 (1000 µl)
Catalog IDs G108
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5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) Applied Biological Materials
Templates mRNA, Total RNA Template Amount > 2 µg Time to Sample 32 min for qPCR, 70 min for PCR
From $ 160.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs G492
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2X PCR TaqFast MasterMix Applied Biological Materials
Templates cDNA, Genomic DNA, Plasmid DNA Fidelity No proofreading activity Amplicon Size < 12 kb
From $ 90.00 (200 Reactions)
Sizes 2 (200 Reactions)
Catalog IDs G280, G280-dye
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Tissue Ex-Amp PCR Kit (with Kodaq MasterMix) Applied Biological Materials
Templates Genomic DNA Fidelity Proofreading activity Amplicon Size /
From $ 85.00 (120 Reactions)
Sizes 1 (120 Reactions)
Catalog IDs G927
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PCR Kits and Methods

cDNA Synthesis

cDNA synthesis is the process of synthesizing DNA from messenger RNAs (mRNAs).

Real-Time PCR

Real-time PCR is a technique that monitors DNA amplification in real-time rather than at the end of the PCR run, as is the case for End-Point PCR.

End-Point PCR

PCR enables amplification of specific DNA fragments with the help of a thermocycler and is a widespread molecular biology technique.

Molecular Cloning

Cloning invovles the assembling of recombinant DNA in a vector, typically a plasmid vector, to express target genes in a host organism.

Competent Cells

Processes like molecular cloning require DNA/RNA transfer amongst cells; the ability for foreign DNA uptake is called competency.

PCR Reagents

PCR requires five reagents; DNA/RNA template, DNA polymerase, primers (forward and reverse), dNTPs and PCR buffers.

The benefit of PCR

Another benefit of the PCR method is its high sensitivity; theoretically, a single copy of the template is enough to start the reaction. The sensitivity and speed make the PCR techniques useful in both basic research and for commercial applications like in vitro diagnostics, forensics, and quality control.

The basic principles of the PCR method

To start a PCR reaction the following is needed:

  • A template: a DNA or RNA strand that we want to amplify.
  • Nucleotides (dCTP, dGTP, dATP, dGTP): the building blocks from which DNA is made.Primers: short nucleotide strands that will anneal to the position that we want to start and end the amplification.
  • DNA polymerase: the enzyme catalyzing the reaction.
  • (Accessory additives like buffers and Mg2+)

Our cDNA synthesis Troubleshoot will guide you though any initial setbacks.

The amplification process:

  • Denaturation: the DNA molecule is split into two single strands of DNA by heating (carried out in a PCR thermocycler)
  • Hybridization: During a cool-down phase, the primers anneal to the target sequences of the single stranded DNA
  • Elongation: Polymerase extends the DNA molecule starting at the primer sequences, and so doubles the template numbers
  • The process is repeated from Step 1 achieving an exponential increase in the number of templates until the substrate runs out or the product accumulation is halting the reaction.

Check out our End-Point PCR Troubleshoot if you encounter any problems.

The past, present and future of PCR

PCR was originally developed to detect specific DNA sequences in a sample, and though this is still the most used application, many other applications have been developed over the past decades.
Nowadays there exist different types of PCRs e.g. End-Point PCR, qPCR (quantitative Real-time PCR), RT-PCR (Reverse Transcription PCR), Multiplex PCR, Nested PCR and Digital PCR. Depending on the polymerases used, the PCR is featured with special properties like a hot start mechanism or proofreading activity to enable the best results.

See our Real-Time PCR Troubleshoot as well as the other troubleshooting guides for tips to perfect your experiment.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Video and Image Credits:

Video: DNA Learning Center/YouTube