Many in vitro studies involve cellular assays at one point another. Therefore cells in the forms of cell lines or primary cells are required by many researchers in their investigations. However, cultivation to the correct density or volume is necessary before performing effectual experiments.
Primary Cells & Cell Lines
Primary cells are isolated from tissues and proliferated until the point of confluence. Primary cells are sub-cultured to produce cell lines. Continuous cell lines can proliferate indefinitely whereas finite cell lines have a limited lifespan. Finite cells can be transformed which renders them immortal. Transformation can occur spontaneously, or it can be virally or chemically-induced.
Histology is the study of microscopic cell, tissue, and organ structures, predominantly with either electron or light microscopes. Histological sections must be stainedtovisualize the cells and create contrast. Various staining methods are used depending on the sample to be stained, with the most common staining system being Haemotoxylin & Eosin.