Cell Line Applications
Cell lines are populations of cells from multicellular organisms that have evaded natural cellular senescence, enabling them to grow for prolonged periods of time in vitro. These characteristics can arise naturally or can be introduced intentionally through artificial modifications for experimental purposes. Continuous cell lines can proliferate indefinitely, whereas finite cell lines are only able to divide 20-100 times before senescence.
Cell lines have arguably revolutionized biological research where they are frequently used in the place of primary cells. These easy-to-handle, widely published cells are often used as simple models for more complex biological systems. Cell lines have a broad range of applications, spanning from studying the standard physiology of cells to observing the effects of drugs and toxins. In addition to being cost-effective, cell lines also avoid the ethical concerns that frequently accompany the use of animal and human tissue in biological research.
Considerations and Common Lines
Despite their popularity and extensive use, cell lines should be utilized with caution. Genetic manipulation and serial passage of cell lines, in addition to genetic drift, can cause genotypic and phenotypic variation within populations. Consequently, cell lines risk losing the characteristics of the original tissue from which they were derived. Furthermore, cell lines can become cross-contaminated with other cell lines and mycoplasma, resulting in extensive alterations in cell behavior and gene expression.
As a result, it is worth taking the time to search for the most appropriate cell line from the best provider for your specific experiment, as some cell lines may be of lower quality and may differ from their described phenotype. V the findings observed with cell lines by using primary cells as control experiments simultaneously.
Here are some examples of commonly used cell lines that are available on ZAGENO:
• HeLa cells are the oldest and most widely used cell line. They were originally derived from cervical cancer cells obtained from Henrietta Lacks. The uses of HeLa cells have been extensive, including playing a pivotal role in the development of the Salk polio vaccine.
• HEK293 cells were originally obtained from human embryonic kidney cells. These cells are readily transfected and are frequently used to produce therapeutic proteins and viruses for gene therapy.
• The Jurkat cell line consists of immortalized human T lymphocyte cells. Jurkat cells are predominantly used to study T cell signaling, acute T-cell leukemia, and the differing susceptibility of cancers to drugs and radiation.
• 3T3 cells are the standard fibroblast cell line. They are frequently used to cultivate keratinocytes due to the growth factors that they secrete.
• Vero cells were isolated from kidney epithelial cells extracted from an African green monkey. Applications of Vero cells include acting as host cells for eukaryotic parasites, and for growing viruses.