Primary Cell Applications
Primary cells are directly isolated from living tissues and established for growth in vitro. Despite their finite lifespan and high sensitivity to growing conditions, they are becoming increasingly popular amongst the biological research community. Once isolated, the cells are cultured under appropriate conditions with the addition of enzymes, growth factors and essential nutrients until they reach adequate numbers. Adherent, or anchorage-dependent primary cells, require attachment for growth and are typically obtained from the tissues of organs. Suspension or anchorage-independent cells are isolated from blood systems and do not require attachment for growth. The applications of primary cells are vast, encompassing virology, vaccine production, genetic engineering and tissue/organ replacement.
Primary Cell Behavior
Since primary cells have undergone relatively few proliferations in comparison to cell lines, they are frequently more representative of the tissue from which they were derived. As a result, they can provide more physiologically significant results than cell lines and are believed to be more biologically relevant tools for studying human and animal biology.
Due to variability amongst the donors of primary cells, the cells can demonstrate variability in their behavior. Therefore, it is important to take the time to decide on the most appropriate population of primary cells for your experiment.