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Immuno-Fluore™ Mounting Medium
Applicable Processes
Immunofluorescence
Immunofluorescence
pH Range
/
Density
/
Scientific Score
7.43
Brand
MP Biomedicals
Sizes
1 (25 ml)
Available From
1 Vendor
Price
$ 364.72
Catalog IDs
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Leit-C SKU : 09929
Applicable Processes
/
pH Range
/
Density
/
Scientific Score
8.62
Brand
Sigma-Aldrich
Sizes
1 (30 g)
Available From
1 Vendor
Price
$ 48.20
Catalog IDs
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LR Gold embedding kit SKU : 10972
Applicable Processes
/
pH Range
/
Density
/
Scientific Score
6.32
Brand
Sigma-Aldrich
Sizes
1 (1 Unit)
Available From
1 Vendor
Price
$ 257.00
Catalog IDs
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Antifade mounting medium (hardset)
Applicable Processes
/
pH Range
/
Density
/
Scientific Score
1.78
Brand
Enzo Life Sciences
Sizes
1 (10 ml)
Available From
1 Vendor
Price
$ 74.76
Catalog IDs
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Mounting Medium SKU : 1211-20
Applicable Processes
/
pH Range
/
Density
/
Scientific Score
2.17
Brand
BioVision
Sizes
1 (20 ml)
Available From
1 Vendor
Price
$ 143.17
Catalog IDs
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Brand
Rockland Immunochemicals
Sizes
1 (10 ml)
Available From
1 Vendor
Price
$ 49.64
Catalog IDs
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Brand
Boster Bio
Sizes
1 (50 ml)
Available From
1 Vendor
Price
$ 30.00
Catalog IDs
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Antifade Mounting Medium SKU: AR1109
Scientific Score
0.36
Brand
Boster Bio
Sizes
1 (10 ml)
Available From
1 Vendor
Price
$ 15.00
Catalog IDs
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Brand
Biospes
Sizes
1 (10 ml)
Available From
1 Vendor
Price
$ 25.00
Catalog IDs
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Brand
Biospes
Sizes
1 (10 ml)
Available From
1 Vendor
Price
$ 25.00
Catalog IDs
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Brand
Biospes
Sizes
1 (10 ml)
Available From
1 Vendor
Price
$ 25.00
Catalog IDs
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Ecomount
Applicable Processes
/
pH Range
/
Density
/
Scientific Score
8.88
Brand
Biocare Medical
Sizes
1 (100 ml)
Available From
1 Vendor
Price
$ 123.00
Catalog IDs
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Mounting Media Applications

Mounting media holds the sample in place during imaging, prevent the sample from drying out or becoming photobleached, and maintain and preserve the sample for short or long-term storage. Mounting media can also change the refractive index of the space between the objective and the glass coverslip so that it is more closely matched to the refractive index of the glass coverslip itself, providing a better image. The two main types of mounting media are broadly classified as water or solvent-based.

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Water-based Media

The most common type of water-based media is buffered saline solutions, such as PBS. The use of water-based media requires few post-processing steps after antibody labeling and staining, enabling rapid sample imaging. If you choose a water-based mounting medium, you may also decide to use a sealant, e.g. melted paraffin wax, to create a moisture barrier and facilitate adhesion of the coverslip to the slide.

Solvent-based Media

Unlike water-based mounting media, solvent-based media require samples to be dehydrated before being transferred to the mounting medium. Despite these additional steps, solvent-based media can preserve samples for the longest. The most appropriate choice of mounting media depends on the sample type to be analyzed and the technique of imaging utilized.