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Vinylcyclohexene dioxide SKU : 94956
Scientific Score
7.70
Brand
Sigma-Aldrich
Sizes
2 (100 - 250 ml)
Available From
2 Vendors
Price Range
$ 441.00 - $ 1,284.85
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CC/Mount™ SKU : c9368
Scientific Score
7.50
Brand
Sigma-Aldrich
Sizes
1 (30 ml)
Available From
2 Vendors
Price Range
$ 72.37 - $ 82.00
Catalog IDs
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CoverGrip Coverslip Sealant
Scientific Score
4.18
Brand
Biotium
Sizes
1 (15 ml)
Available From
1 Vendor
Price
$ 36.98
Catalog IDs
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DAPI Fluoromount-G®
Applicable Processes
/
pH Range
/
Density
/
Scientific Score
4.64
Brand
Southern Biotechnology
Sizes
1 (20 ml)
Available From
1 Vendor
Price
$ 52.31
Catalog IDs
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Fluoroshield™ with PPD
Scientific Score
4.28
Brand
Sigma-Aldrich
Sizes
1 (20 ml)
Available From
2 Vendors
Price Range
$ 69.00 - $ 76.50
Catalog IDs
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Brand
Sigma-Aldrich
Sizes
1 (20 ml)
Available From
1 Vendor
Price
$ 81.40
Catalog IDs
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Histochoice Mounting Media SKU: H157
Scientific Score
2.57
Brand
AMRESCO
Sizes
2 (120 - 475 ml)
Available From
1 Vendor
Price Range
$ 59.66 - $ 68.27
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Rapid Freeze™ Spray SKU: RAP-FRZ
Applicable Processes
Sample preparation
Sample preparation
pH Range
/
Density
/
Scientific Score
4.64
Brand
Azer Scientific
Sizes
1 (12 Units)
Available From
2 Vendors
Price Range
$ 133.65 - $ 374.18
Catalog IDs
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Rapid Freeze™ Spray SKU: RAP-ZAP
Applicable Processes
Sample preparation
Sample preparation
pH Range
/
Density
/
Scientific Score
6.25
Brand
Azer Scientific
Sizes
1 (1 Unit)
Available From
1 Vendor
Price
$ 16.32
Catalog IDs
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Brand
Vector Laboratories
Sizes
1 (10 ml)
Available From
2 Vendors
Price Range
$ 179.00 - $ 186.75
Catalog IDs
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Immuno-Fluore™ Mounting Medium
Applicable Processes
Immunofluorescence
Immunofluorescence
pH Range
/
Density
/
Scientific Score
7.43
Brand
MP Biomedicals
Sizes
1 (25 ml)
Available From
1 Vendor
Price
$ 364.72
Catalog IDs
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Mounting Medium
Applicable Processes
/
pH Range
/
Density
/
Scientific Score
2.07
Brand
R&D Systems
Sizes
1 (15 ml)
Available From
1 Vendor
Price
$ 45.00
Catalog IDs
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Mounting Media Applications

Mounting media holds the sample in place during imaging, prevent the sample from drying out or becoming photobleached, and maintain and preserve the sample for short or long-term storage. Mounting media can also change the refractive index of the space between the objective and the glass coverslip so that it is more closely matched to the refractive index of the glass coverslip itself, providing a better image. The two main types of mounting media are broadly classified as water or solvent-based.

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Water-based Media

The most common type of water-based media is buffered saline solutions, such as PBS. The use of water-based media requires few post-processing steps after antibody labeling and staining, enabling rapid sample imaging. If you choose a water-based mounting medium, you may also decide to use a sealant, e.g. melted paraffin wax, to create a moisture barrier and facilitate adhesion of the coverslip to the slide.

Solvent-based Media

Unlike water-based mounting media, solvent-based media require samples to be dehydrated before being transferred to the mounting medium. Despite these additional steps, solvent-based media can preserve samples for the longest. The most appropriate choice of mounting media depends on the sample type to be analyzed and the technique of imaging utilized.