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Mounting Media

VECTASHIELDR Antifade Mounting Medium SKU: H-1000-NB

Novus Biologicals

From
$ 149.00 (10 ml)
Sizes
1 (10 ml)
Catalog IDs
H-1000-NB
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Mounting Media

VECTASHIELDR HardSetTM Antifade Mounting Medium with DAPI SKU: H-1500-NB

Novus Biologicals

From
$ 179.00 (10 ml)
Sizes
1 (10 ml)
Catalog IDs
H-1500-NB
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Mounting Media

Anti-fade Mounting Medium (With PI) SKU: BAR1002

Biospes

From
$ 25.00 (10 ml)
Sizes
1 (10 ml)
Catalog IDs
BAR1002-10ML
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Mounting Media

CC/Mount™ SKU : c9368

Sigma-Aldrich

From
$ 72.37 (30 ml)
Sizes
1 (30 ml)
Catalog IDs
C9368-30ML
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Mounting Media

Histochoice Mounting Media SKU: H157

AMRESCO

From
$ 59.66 (120 ml)
Sizes
2 (120 - 475 ml)
Catalog IDs
H157-120ML, H157-475ML
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Mounting Media

Mounting Medium

Sigma-Aldrich

From
$ 34.25 (10 ml)
Sizes
1 (10 ml)
Catalog IDs
M1289-10ML
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Mounting Media

Durcupan™ ACM SKU : 44612

Sigma-Aldrich

Applicable Processes
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From
$ 166.00 (500 ml)
Sizes
1 (500 ml)
Catalog IDs
44612-500ML
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Mounting Media

Durcupan™ water-soluble SKU : 44591

Sigma-Aldrich

Applicable Processes
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/
From
$ 29.75 (100 ml)
Sizes
1 (100 ml)
Catalog IDs
44591-100ML
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Mounting Media

D.E.R.™ 736 SKU : 31191

Sigma-Aldrich

Applicable Processes
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/
From
$ 34.50 (250 g)
Sizes
2 (1 - 250 kg)
Catalog IDs
31191-1KG, 31191-250G
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Mounting Media

DPX Mountant for histology SKU : 06522

Sigma-Aldrich

Applicable Processes
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From
$ 27.83 (100 ml)
Sizes
2 (100 - 500 ml)
Catalog IDs
06522-100ML, 06522-500ML
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Mounting Media

Leit-C SKU : 09929

Sigma-Aldrich

Applicable Processes
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From
$ 48.20 (30 g)
Sizes
1 (30 g)
Catalog IDs
09929-30G
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Mounting Media

SignalStain® Mounting Medium

Cell Signaling Technology

Applicable Processes
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From
$ 132.00 (60 ml)
Sizes
1 (60 ml)
Catalog IDs
14177S
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Mounting Media Applications

Mounting media holds the sample in place during imaging, prevent the sample from drying out or becoming photobleached, and maintain and preserve the sample for short or long-term storage. Mounting media can also change the refractive index of the space between the objective and the glass coverslip so that it is more closely matched to the refractive index of the glass coverslip itself, providing a better image. The two main types of mounting media are broadly classified as water or solvent-based.

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Water-based Media

The most common type of water-based media is buffered saline solutions, such as PBS. The use of water-based media requires few post-processing steps after antibody labeling and staining, enabling rapid sample imaging. If you choose a water-based mounting medium, you may also decide to use a sealant, e.g. melted paraffin wax, to create a moisture barrier and facilitate adhesion of the coverslip to the slide.

Solvent-based Media

Unlike water-based mounting media, solvent-based media require samples to be dehydrated before being transferred to the mounting medium. Despite these additional steps, solvent-based media can preserve samples for the longest. The most appropriate choice of mounting media depends on the sample type to be analyzed and the technique of imaging utilized.