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Mounting Media on ZAGENO

Mounting Medium Sigma-Aldrich
From $ 33.30 (10 ml)
Sizes 1 (10 ml)
Catalog IDs M1289-10ML
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Fisher Chemical™ Permount™ Mounting Medium Fisher Chemical
From $ 42.52 (100 ml)
Sizes 1 (100 ml)
Catalog IDs SP15-100
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FluorFluoromount-G™ Slide Mounting Medium Electron Microscopy Sciences
From $ 80.99 (25 ml)
Sizes 1 (25 ml)
Catalog IDs 17984-25
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Fluoromount™ Aqueous Mounting Medium Sigma-Aldrich
From $ 75.80 (25 ml)
Sizes 1 (25 ml)
Catalog IDs F4680-25ML
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Polyvinyl alcohol mounting medium with DABCO®, antifading Sigma-Aldrich
From $ 124.00 (100 ml)
Sizes 1 (100 ml)
Catalog IDs 10981-100ML
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Duolink® In Situ Mounting Medium with DAPI Sigma-Aldrich
From $ 182.00 (5 ml)
Sizes 1 (5 ml)
Catalog IDs DUO82040-5ML
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ProLong™ Gold Antifade Mountant Invitrogen
From $ 172.00 (10 ml)
Sizes 1 (10 ml)
Catalog IDs P36930
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VECTASHIELD Antifade Mounting Medium Vector Laboratories
From $ 135.00 (10 ml)
Sizes 1 (10 ml)
Catalog IDs H-1000
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ProLong™ Gold Antifade Mountant with DAPI Thermo Scientific
From $ 180.00 (10 mL)
Sizes 4 (1 - 10 Pack)
Catalog IDs P36931, P36935, ...
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Eukitt® Quick-hardening mounting medium Sigma-Aldrich
From $ 44.20 (100 ml)
Sizes 2 (100 - 500 ml)
Catalog IDs 03989-100ML, 03989-500ML
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Mounting Media Applications

Mounting media holds the sample in place during imaging, prevent the sample from drying out or becoming photobleached, and maintain and preserve the sample for short or long-term storage. Mounting media can also change the refractive index of the space between the objective and the glass coverslip so that it is more closely matched to the refractive index of the glass coverslip itself, providing a better image. The two main types of mounting media are broadly classified as water or solvent-based.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Water-based Media

The most common type of water-based media is buffered saline solutions, such as PBS. The use of water-based media requires few post-processing steps after antibody labeling and staining, enabling rapid sample imaging. If you choose a water-based mounting medium, you may also decide to use a sealant, e.g. melted paraffin wax, to create a moisture barrier and facilitate adhesion of the coverslip to the slide.

Solvent-based Media

Unlike water-based mounting media, solvent-based media require samples to be dehydrated before being transferred to the mounting medium. Despite these additional steps, solvent-based media can preserve samples for the longest. The most appropriate choice of mounting media depends on the sample type to be analyzed and the technique of imaging utilized.