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Mounting Media on ZAGENO

Leit-C SKU : 09929 Sigma-Aldrich
From $ 48.20 (30 g)
Sizes 1 (30 g)
Catalog IDs 09929-30G
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Fisher Chemical™ Permount™ Mounting Medium Fisher Chemical
From $ 42.52 (100 ml)
Sizes 1 (100 ml)
Catalog IDs SP15-100
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Durcupan™ ACM SKU : 44612 Sigma-Aldrich
From $ 174.00 (500 ml)
Sizes 1 (500 ml)
Catalog IDs 44612-500ML
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Durcupan™ water-soluble SKU : 44591 Sigma-Aldrich
From $ 28.90 (100 ml)
Sizes 1 (100 ml)
Catalog IDs 44591-100ML
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D.E.R.™ 736 SKU : 31191 Sigma-Aldrich
From $ 32.70 (250 g)
Sizes 2 (1 - 250 kg)
Catalog IDs 31191-1KG, 31191-250G
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Mounting Medium Sigma-Aldrich
From $ 33.30 (10 ml)
Sizes 1 (10 ml)
Catalog IDs M1289-10ML
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FluorFluoromount-G™ Slide Mounting Medium Electron Microscopy Sciences
From $ 80.99 (25 ml)
Sizes 1 (25 ml)
Catalog IDs 17984-25
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Vinylcyclohexene dioxide SKU : 94956 Sigma-Aldrich
From $ 411.50 (100 ml)
Sizes 2 (100 - 250 ml)
Catalog IDs 94956-100ML, 94956-250ML
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Durcupan™ ACM SKU : 44614 Sigma-Aldrich
From $ 38.00 (100 ml)
Sizes 1 (100 ml)
Catalog IDs 44614-100ML
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Araldite® M SKU : 10951 Sigma-Aldrich
From $ 77.60 (250 ml)
Sizes 2 (1 - 250 L)
Catalog IDs 10951-1L, 10951-250ML
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Epoxy embedding medium SKU : 45345 Sigma-Aldrich
From $ 79.60 (250 ml)
Sizes 3 (1 - 500 L)
Catalog IDs 45345-1L-F, 45345-250ML-F, ...
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Fluoromount™ Aqueous Mounting Medium Sigma-Aldrich
From $ 75.80 (25 ml)
Sizes 1 (25 ml)
Catalog IDs F4680-25ML
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Mounting Media Applications

Mounting media holds the sample in place during imaging, prevent the sample from drying out or becoming photobleached, and maintain and preserve the sample for short or long-term storage. Mounting media can also change the refractive index of the space between the objective and the glass coverslip so that it is more closely matched to the refractive index of the glass coverslip itself, providing a better image. The two main types of mounting media are broadly classified as water or solvent-based.

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Water-based Media

The most common type of water-based media is buffered saline solutions, such as PBS. The use of water-based media requires few post-processing steps after antibody labeling and staining, enabling rapid sample imaging. If you choose a water-based mounting medium, you may also decide to use a sealant, e.g. melted paraffin wax, to create a moisture barrier and facilitate adhesion of the coverslip to the slide.

Solvent-based Media

Unlike water-based mounting media, solvent-based media require samples to be dehydrated before being transferred to the mounting medium. Despite these additional steps, solvent-based media can preserve samples for the longest. The most appropriate choice of mounting media depends on the sample type to be analyzed and the technique of imaging utilized.