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Stains & Dyes

ProLong® Gold Antifade Reagent

Cell Signaling Technology

Applicable Processes
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From
$ 172.00 (10 ml)
Sizes
1 (10 ml)
Catalog IDs
9071S
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Stains & Dyes

BROMOCRESOL GREEN, A.C.S. REAGENT

Sigma-Aldrich

Applicable Processes
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From
$ 37.34 (5 g)
Sizes
1 (5 g)
Catalog IDs
114359-5G
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Stains & Dyes

Toluidine Blue O SKU : 198161

Sigma-Aldrich

Applicable Processes
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From
$ 44.07 (5 g)
Sizes
2 (5 - 25 g)
Catalog IDs
198161-5G, 198161-25G
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Stains & Dyes

Folin & Ciocalteu’s phenol reagent SKU : 47641

Sigma-Aldrich

Applicable Processes
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From
$ 50.50 (100 ml)
Sizes
2 (100 - 500 ml)
Catalog IDs
47641-100ML-F, 47641-500ML-F
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Stains & Dyes

Giemsa Stain, Modified Solution

Sigma-Aldrich

Applicable Processes
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From
$ 33.25 (100 ml)
Sizes
1 (100 ml)
Catalog IDs
48900-100ML-F
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Stains & Dyes

Hematoxylin solution according to Mayer

Sigma-Aldrich

Applicable Processes
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From
$ 55.44 (100 ml)
Sizes
3 (100 - 1000 ml)
Catalog IDs
51275-100ML, 51275-500ML, ...
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Stains & Dyes

Eosin B

Sigma-Aldrich

Applicable Processes
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From
$ 40.94 (10 g)
Sizes
1 (10 g)
Catalog IDs
861006-10G
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Stains & Dyes

CONGO RED BIOXTRA, CERTIFIED BY THE BIO&

Sigma-Aldrich

Applicable Processes
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From
$ 124.32 (25 g)
Sizes
1 (25 g)
Catalog IDs
C6277-25G
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Stains & Dyes

Eosin Y disodium salt Dye content ≥85 %

Sigma-Aldrich

Applicable Processes
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From
$ 32.20 (25 g)
Sizes
1 (25 g)
Catalog IDs
E6003-25G
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Stains & Dyes

eBioscience™ CyTRAK Orange™

Invitrogen

Applicable Processes
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/
From
$ 204.00 (50 µl)
Sizes
1 (50 µl)
Catalog IDs
65-0881-92
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Stains & Dyes

Inside Stain Kit

Miltenyi Biotec

From
$ 228.00 (50 Tests)
Sizes
1 (50 Tests)
Catalog IDs
130-090-477
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Stains & Dyes

Annexin V-FITC

Miltenyi Biotec

Applicable Processes
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/
From
$ 51.00 (30 Tests)
Sizes
2 (30 - 100 Tests)
Catalog IDs
130-097-928, 130-093-060
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Stains/Dyes Applications

Stains and dyes play a critical role in tissue-based diagnosis and research. Stains are temporary, whereas dyes are permanent and can only be removed after cell wall destruction. Dyes are organic unsaturated cyclic compounds which are made up of a chromophore and an auxochrome group. The chromophore is responsible for the specific color of the dye, and the auxochrome group causes the dyeing property of the salt formation. Often dyes require the addition of a mordant to improve the fastness of the dye to the substrate. Stains are biological coloring agents that are purer and prepared with greater specifications than dyes. The electrical charges of the chromagen portions of the both the stain and the cellular components determine the ability of the stain to bind to and stain macromolecular cellular components.

Stains and dyes serve multiple purposes in histology and hematology. They provide contrast to biological tissue, enhancing visualization of cells or cellular components and enabling researchers to view tissue morphology and structure. Stains and dyes can be used to examine bulk tissues, cell populations, or organelles within individual cells. Furthermore, stains and dyes can be used to differentiate not only between live and dead cells but also between different types of organisms. Finally, stains and dyes can aid in the detection of certain features, metabolic processes, and cell types or organelles.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Staining Techniques

Before the addition of the stain/dye, a solvent is used to remove wax from the slide in a process called de-waxing. Following completion of staining, a coverglass is added to the section to make the stain permanent.

Below are some examples of popular staining techniques utilized by researchers:

  • Simple staining uses only one dye. Commonly used dyes for simple staining that are available on Zageno include methylene blue, gram safranin, and gram crystal violet.
  • Differential staining uses more than one chemical stain to differentiate between micro-organisms, or structures and cellular components of an individual organism. Gram staining, which is an example of differential staining, uses two stains are to distinguish between gram positive and negative bacteria.
  • Routine staining, which is the most common method of staining, reveals underlying tissue structures and conditions. Routine stains are made up of hematoxylin and eosin (H&E) which are basic and acidic dyes, respectively.
  • Counterstains make the stained structure more visible by staining with a color contrasting to the principle stain.
  • Silver staining is an important technique for visualizing proteins, DNA, and substances inside and outside of cells. This method uses silver to stain histologic sections.
  • Papanicolaou staining is used to examine cell samples from various bodily secretions.
  • Masson’s Trichrome is a three-colour staining method frequently used in histology. It distinguishes cells from surrounding connective tissue.

Stain Selection

An extensive range of stains and dyes are available on Zageno, including the popular choices of Coomassie brilliant blue, DAPI and ethidium bromide solution. When selecting the most appropriate dye for your experiment, remember that the composition of dyes frequently varies between suppliers. Most dyes are available as certified strains, meaning that following extensive testing, they have been found to reach and exceed expectations of dye content, purity, and performance in staining methodologies. The use of certified stains is recommended as it reduces the likelihood of obtaining unexpected and inaccurate results.