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N-Tetradecyl-N,N-Dimethyl-3-Ammonio-1-Propanesulfonate (SB3-14, Myristyl Sulfobetaine) SKU: T2967
Applicable Processes
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CAS Number
14933-09-6
14933-09-6
Chemical Name
/
Brand
US Biological
Sizes
2 (10 - 25 g)
Available From
1 Vendor
Price Range
$ 403.00 - $ 701.00
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ASB-16 (3-[N,N-Dimethyl(3-palmitoylaminopropyl)ammonio]propanesulfonate; Amidosulfobetaine-16) SKU: 169934
Applicable Processes
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CAS Number
52562-29-5
52562-29-5
Chemical Name
/
Brand
US Biological
Sizes
2 (1 - 5 g)
Available From
1 Vendor
Price Range
$ 273.00 - $ 479.00
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N,N-Bis[3-D-gluconamidopropyl]cholamide (Ultra Pure) SKU: 004173
Applicable Processes
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CAS Number
86303-22-2
86303-22-2
Chemical Name
/
Brand
US Biological
Sizes
1 (1 g)
Available From
1 Vendor
Price
$ 425.00
Catalog IDs
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Sodium Lauryl Sulfate NF
Applicable Processes
/
CAS Number
151-21-3
151-21-3
Chemical Name
/
Scientific Score
9.84
Brand
Oakwood Chemical
Sizes
5 (0.025 - 10 kg)
Available From
1 Vendor
Price Range
$ 10.00 - $ 355.00
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Tergitol® Xj, Surfactant SKU: T1312
Applicable Processes
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CAS Number
9038-95-3
9038-95-3
Chemical Name
/
Scientific Score
7.15
Brand
Spectrum Chemical
Sizes
1 (100 ml)
Available From
1 Vendor
Price
$ 79.05
Catalog IDs
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Tergitol® Xh, Surfactant SKU: T1309
Applicable Processes
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CAS Number
9038-95-3
9038-95-3
Chemical Name
/
Scientific Score
7.15
Brand
Spectrum Chemical
Sizes
1 (100 ml)
Available From
1 Vendor
Price
$ 130.20
Catalog IDs
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Triton® X-45 SKU: TR125
Applicable Processes
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CAS Number
9002-93-1
9002-93-1
Chemical Name
/
Scientific Score
7.93
Brand
Spectrum Chemical
Sizes
4 (4 - 500 L)
Available From
2 Vendors
Price Range
$ 163.80 - $ 4,019.95
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Tergitol® Tmn-10 (90% Aqueous), Surfactant SKU: T1288
Applicable Processes
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CAS Number
60828-78-6
60828-78-6
Chemical Name
/
Scientific Score
8.16
Brand
Spectrum Chemical
Sizes
6 (2.5 - 500 L)
Available From
2 Vendors
Price Range
$ 81.90 - $ 3,628.30
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Tergitol® Tmn-6 (90% Aqueous), Surfactant SKU: T1303
Applicable Processes
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CAS Number
60828-78-6
60828-78-6
Chemical Name
/
Scientific Score
7.93
Brand
Spectrum Chemical
Sizes
4 (2.5 - 200 L)
Available From
2 Vendors
Price Range
$ 81.90 - $ 3,628.30
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Polyoxyl 40 Stearate, NF SKU: P1159
Applicable Processes
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CAS Number
9004-99-3
9004-99-3
Chemical Name
/
Scientific Score
8.08
Brand
Spectrum Chemical
Sizes
5 (12 - 2500 kg)
Available From
2 Vendors
Price Range
$ 125.60 - $ 5,016.90
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Cocamidopropyl Betaine SKU: C3493
Applicable Processes
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CAS Number
/
Chemical Name
/
Scientific Score
4.92
Brand
Spectrum Chemical
Sizes
3 (1 - 200 L)
Available From
1 Vendor
Price Range
$ 207.35 - $ 1,241.25
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N, N-Dimethyldodecylamine SKU: D1662
Applicable Processes
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CAS Number
112-18-5
112-18-5
Chemical Name
/
Scientific Score
7.52
Brand
Spectrum Chemical
Sizes
2 (250 - 1000 g)
Available From
1 Vendor
Price Range
$ 58.25 - $ 160.80
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Detergent Function & Application

Detergents effectively solubilize hydrophobic molecules; they are small amphipathic molecules consisting of a long alkyl chain (hydrophobic) tail attached to hydrophilic/ water solubility enhancing functional groups (a polar head). Examples include sodium dodecyl sulfate, CHAPS, and polyethylene glycol.

Applications of detergents include liposome preparation, cell lysis, analyte precipitation in solutions, solubilization of proteins and lipids, DNA & RNA extraction, as well as preventing non-specific binding in immunoassays and westerns.

The detergent type depends on the charge of the head group, whether it is ionic, non-ionic or zwitterionic.

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Anionic Detergents

Anionic Detergents have a negative charge and are used in DNA or RNA purification, due to their ability to disrupt cell membranes and denature proteins. However, these detergents are sensitive to ionic strength, pH and counterion nature. They may also interfere with charge-based analytical methods. Examples include:

• Sodium cholate

• Sodium deoxycholate

• Sodium glycocholate

• Sodium taurocholate

• Sodium Taurodeoxycholate

• N-lauryl sarcosine (Sarkosyl solution)

• Lithium dodecyl sulfate (LDS)

• Sodium dodecyl sulfate (SDS) (a vital component of SDS-PAGE)

Cationic Detergents

Cationic Detergents have a positive charge and are therefore useful at residual anionic detergents. They can be used as surfactants in drug or vaccine delivery, while also applying to DNA extraction. Due to being characteristically germicidal, they may also be used as disinfectants. Examples include CTAB (hexadecyltrimethylammonium bromide) and TTAB (Trimethyltetradecyl ammonium bromide).

Non-ionic Detergents

Non-ionic detergents are milder and therefore less likely to denature proteins, however shorter hydrophobic chain lengths may cause some denaturation.

• Poly (oxyethylene) ethers (& related polymers) have hydrophobic tails and a neutral, polar head group. Popular oxyethylene polymers are Tween 20 and Tween 80

• Bile Salts have a steroid core with a polar or apolar structure; they are less denaturing than similar detergents

• Glycosidic Detergents have a carbohydrate (glucose/maltose) polar head group and a nonpolar tail of between 7-14 carbons.

Zwitterionic Detergents

Zwitterionic detergents have a neutral charge and are therefore also less denaturing; critical applications are disrupting protein-protein bonds and reducing aggregation. These assets are put to good use in mass spectrometry, chromatography, electrophoresis and solubilization of cellular organelles and inclusion bodies.