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Enzymes

Score 1.39

ACTaq™ High Fidelity DNA Polymerase

ACTGene

From
$ 66.39 (1 Unit)
Sizes
2 (1 Unit)
Catalog IDs
E2000-1000U, E2000-250U
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Enzymes

Score 7.86

110 Klentaq-LA enzyme only

DNA Polymerase Technology

From
$ 225.00 (100 μl)
Sizes
1 (100 μl)
Catalog IDs
110
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Enzymes

Score 4.95

Zymolyase®-20T

Sunrise Science Products

From
$ 195.00 (1 g)
Sizes
1 (1 g)
Catalog IDs
N0766391
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Enzymes

Score 1.54

SwaI

Applied Biological Materials

From
$ 135.00 (10000 Units)
Sizes
1 (10000 Units)
Catalog IDs
E061
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Enzymes

Score 8.41

CDK1/CycB1

ProQinase

From
$ 265.00 (10 μg)
Sizes
2 (10 - 100 μg)
Catalog IDs
0134-0135-1-10UG, 0134-0135-1-100UG
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Enzymes

Score 1.42

Cap-Clip™ Acid Pyrophosphatase

CellScript

From
$ 550.00 (100 Units)
Sizes
1 (100 Units)
Catalog IDs
C-CC15011H
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Enzymes

Score 7.16

β-Glucuronidase from limpets (Patella vulgata) SKU : g2174

Sigma-Aldrich

From
$ 60.00 (1 ml)
Sizes
4 (1 - 25 ml)
Catalog IDs
G2174-1ML, G2174-5ML, ...
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Enzymes

Score 9.22

Furin human SKU : f2677

Sigma-Aldrich

Enzyme Type
/
/
Enzyme Concentration
/
/
Templates
/
/
From
$ 315.00 (50 UN)
Sizes
1 (50 UN)
Catalog IDs
F2677-50UN
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Enzymes

Score 9.11

β-Glucosidase from almonds SKU : g4511

Sigma-Aldrich

Enzyme Type
/
/
Enzyme Concentration
/
/
Templates
/
/
From
$ 139.00 (100 UN)
Sizes
3 (1 - 250 KU)
Catalog IDs
G4511-1KU, G4511-100UN, ...
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Enzymes

Score 5.79

Phosphoglucose Isomerase from baker's yeast (S. cerevisiae) SKU : f2668

Sigma-Aldrich

From
$ 62.00 (0.4 ml)
Sizes
1 (0.4 ml)
Catalog IDs
F2668-.4ML
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Enzymes

Score 8.66

FLT1 (784-end), active, GST tagged human SKU : f6307

Sigma-Aldrich

Enzyme Type
/
/
Enzyme Concentration
/
/
Templates
/
/
From
$ 391.00 (10 µg)
Sizes
1 (10 µg)
Catalog IDs
F6307-10UG
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Enzymes

Score 8.66

FLT3 (571-993), active, GST tagged human SKU : f6432

Sigma-Aldrich

Enzyme Type
/
/
Enzyme Concentration
/
/
Templates
/
/
From
$ 373.00 (10 µg)
Sizes
1 (10 µg)
Catalog IDs
F6432-10UG
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Enzymes are biological catalysts present in all cells enabling chemical reactions to proceed at a fast enough rate so as to sustain life. Thus, enzymes are essential to almost all molecular biology processes, not only within our bodies, but also those carried out in the lab, ranging from purification to gene editing and sequencing.

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The basics of enzymes

Enzymes lower the activation energy of a reaction, thus accelerating the reaction rate of the conversion of substrate to product. The increased rate can be by a factor of several million and without this increased reaction rate, life would not be able to be sustained here on Earth.
Most enzymes are proteins, but there are a few exceptions where RNA molecules can act as catalysts for reactions.
Since the enzyme is a catalyst, it is not consumed during the reaction. Thus, decreased reaction rates observed over time is not due to the enzyme concentration decreasing, but a combination of reduced availability of substrates and an accumulation of product.
Enzymes often work within very narrow pH and temperature ranges. Hence, it is essential that you use the correct buffers and optimal temperature ranges given in the protocol that is supplied with the enzyme.
Enzymes can lower the activation energy (ΔG‡, Gibbs free energy) of a reaction in several ways.

  • Stabilization of the transition state

  • Providing alternative pathways

  • Destabilization of the substrate ground state

The nomenclature of enzymes is determined by the substrate that is used in the reaction and with the addition of the suffix -ase. One example of this would be the alcohol-metabolizing enzyme alcohol dehydrogenase.
Generally, enzymes are divided into the following groups:

Oxidoreductases: catalyze oxidation/reduction reactions

Ligases: can join molecules with covalent bonds

Transferases: can transfer functional groups, such as polymerases

Isomerases: can catalyze isomerization changes

Hydrolases: catalyze the hydrolysis of various bonds, including restriction enzymes

Lyases: cleave various bonds (not hydrolysis and oxidation)

Isozymes is the name given to enzymes that are different, but are catalyzing the same chemical reaction.

Our Restriction Enzyme Troubleshoot is useful for solving any potential problems.

Compare Enzymes

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.