ZAGENO
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Detection on Zageno

OneQuant™ FITC G-Biosciences
Provided Label/Dye Fluorescent dye Label or Dye (specific) FITC Labeling Target Antibodies, Proteins
From $ 50.00 (8 mg)
Sizes 1 (8 mg)
Catalog IDs 786-080
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ONPG kit OZ Biosciences
Quantification Method β-Galactosidase Assay Targets / Sensitivity /
From $ 207.00 (500 Reagents)
Sizes 1 (500 Reagents)
Catalog IDs GO10001
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Senescence Kit OZ Biosciences
Sample Type Cells Organism Animals Measurement of ß-Galactosidase activity
From $ 171.00 (50 Reactions)
Sizes 1 (50 Reactions)
Catalog IDs GXS0003
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Luciferase Assay kit OZ Biosciences
Quantification Method Luciferase Assay Targets / Sensitivity /
From $ 100.00 (100 Reactions)
Sizes 2 (100 - 1000 Reactions)
Catalog IDs LUC0100, LUC1000
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CasPASE™ 1, 4, 5 assay with Ac-WEHD-AFC substrate G-Biosciences
Sample Type Lysate Organism Animals Measurement of Caspase activity, Protease activity
From $ 401.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs 786-200B
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Total Histone H3 ELISA Active Motif
Measurement of Proteins Sample Type / ELISA Type /
From $ 525.00 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs 53110
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hTERT Cell Immortalization Kit Alstem
Target Molecules / Sample Type Cells Organism Animals
From $ 1,250.00 (100 µl)
Sizes 1 (100 µl)
Catalog IDs CILV02
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Cell Viability Kit BD
Target Molecules Cells Sample Type Cells Organism Bacteria, Animals, Yeast
From (on request)
Sizes 1 (100 Reactions)
Catalog IDs 349483
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ViPrimePLUS Hepatitis B Virus qPCR Kit Vivantis
Detection of Human pathogen Pathogen Type Virus Sample Type Plasma & serum
From (on request)
Sizes 1 (150 Reactions)
Catalog IDs QM5011
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VetMAX™-Gold BVDV PI Detection Kit Applied Biosystems
Detection of Animal pathogen Pathogen Type Virus Sample Type Mammalian cells, Tissue
From $ 590.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs 4413938
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HDAC Fluorogenic Assay Kit (Green) BPS Bioscience
Sample Type / Organism / Measurement of Other activity
From $ 405.00 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs 50034
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DNMT1 Chemiluminescent Assay Kit BPS Bioscience
Sample Type Extract Organism / Measurement of Other activity
From $ 770.00 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs 52050L
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Detection Kits and Methods

Detection Kits

Detection procedures involve extracting and purifying nucleic acids following an agarose gel electrophoresis for identification purposes.

Quantification

Quantification of the total amount of sample is a necessary first step during most analyses.

ELISA

ELISA detects particles using antibodies and a simple enzymatic color change. Types include Direct or Indirect, Sandwich, and Competitive ELISA.

Molecular Labeling

Labeling can identify the location of molecules within the cell or the genome as well as determine their presence or concentration.

Enzyme Assays

Enzyme assays measure enzymatic activity within the cell, using detective methods like fluorescence, luminescence or colorimetry.

Microarrays

Microarrays is a lab-on-chip device allow scientists to investigate the expression of thousands of genes in multiple samples simultaneously.

Pathogen Detection

Pathogen detection is essential in clinical microbiology and the food industry, it allows proper treatment and prevention of illness.

Protein Quantification

Quantification of protein concentration is a necessary first step during any protein analysis. This can be achieved by UV absorbance or HPLC

Blotting Reagents

Blotting reagents are essential in western, northern and southern blotting for detection of proteins and nucleic acids.

Detection kits

Depending on what your sample is (RNA, DNA, protein or tissue), the method of detection for your target molecule will vary. The organism that the molecule originated from can also determine which kit you will require. For example, detecting a viroid in plants will involve different procedures compared to testing whether E.coli is present in a water sample. If you need to detect whether a certain bacterium or virus is within your test subject, there are certain specialized kits for that. On top of that, there are kits that are developed for certain sample types such as; urine, cell culture, blood, as well as food.

Quantification and Protein Quantification

Quantification determines the concentration of proteins or nucleic acids within a sample, and is an essential step before further analysis such as Western blotting or sequencing. These techniques usually require a minimum amount of protein / nucleic acid to be loaded for testing. It is often best to perform quantification methods prior to protein purification, as the outcome of the downstream methods can greatly vary depending on protein concentration.

Check out or Bradford Assay Troubleshoot for more assistance in this area.

ELISA

ELISA kits help determine which specific nucleic acids/proteins are within a sample via the use of specific complementary antibodies. The assay can analyze the sample (either qualitatively or quantitatively); utilizing serum, plasma, or tissues. In comparison with Western blotting, ELISA is simpler and quicker but requires the antibody to be much more specific. ELISA also mainly works on native proteins, whilst Westerns works on denatured proteins (and identifies the purity of your proteins).

The ELISA Troubleshoot contains further information regarding potential errors.

Molecular Labeling

For processes such as ELISA, labeling processes are necessary to determine whether the whole procedure is successful. More specifically, labeling nucleic acids and proteins can identify the position of these molecules within the cell or the genome as well as determine their presence or concentration.

You can explore our Antibody Labeling Troubleshoot for more guidance.

Enzyme Assays

Enzymes are specialized proteins or in some cases RNA molecules which speed up reactions in the cell. Given an enzyme’s ability to catalyze a reaction, they are vital for cellular processes such as signaling pathways, metabolism, and gene expression. Thus in knowing enzyme activity within the cell, scientists can infer the mechanism behind reactions.