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Blotting Reagents on Zageno

Restore™ Western Blot Stripping Buffer Thermo Scientific
Reagent Type / Blot Type / Detection Technique /
From $ 231.93 (500 ml)
Sizes 1 (500 ml)
Catalog IDs PI21059
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PVDF Transfer Membrane, 0.45 µm, 10 cm x 10 cm Thermo Scientific
Reagent Type / Blot Type / Detection Technique /
From (on request)
Sizes 1 (10 Sheets)
Catalog IDs 88585
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Novex® AP Rabbit Chemiluminescent Detection Kit Novex
Reagent Type Detection reagents Blot Type Western blot Detection Technique Luminescence
From $ 109.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs SLF1022
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Easy WESTERN II Super, mouse enhancer set Cosmo Bio
Reagent Type Detection reagents Blot Type Western blot Detection Technique Fluorescence
From $ 440.00 (50 Reactions)
Sizes 1 (50 Reactions)
Catalog IDs BEC-BCL-EZS24
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Immun-Blot® PVDF Membrane, Precut, 10 x 15 cm Bio-Rad
Reagent Type Blotting membranes Blot Type Western blot Detection Technique Colorimetry, Luminescence
From $ 125.00 (10 Membranes)
Sizes 1 (10 Membranes)
Catalog IDs 1620175
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BLOK™ BSA in TBS [10X] G-Biosciences
Reagent Type Blocking reagents Blot Type Dot blot, Western blot Detection Technique /
From $ 145.00 (125 ml)
Sizes 1 (125 ml)
Catalog IDs 786-193
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Nitrocellulose Membrane, 0.2 µm, 7.9 cm x 10.5 cm Thermo Scientific
Reagent Type / Blot Type / Detection Technique /
From $ 123.00 (15 Membranes)
Sizes 1 (15 Membranes)
Catalog IDs 88013
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Novex® AP Mouse Chemiluminescent Detection Kit Novex
Reagent Type Detection reagents Blot Type Western blot Detection Technique Luminescence
From $ 109.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs SLF1021
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EZ-ECL Kit Biological Industries
Reagent Type Detection reagents Blot Type Northern blot, Southern blot, Western blot Detection Technique Luminescence
From (on request)
Sizes 2 (120 - 500 ml)
Catalog IDs 20-500-120, 20-500-500
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QC Colloidal Coomassie Stain Bio-Rad
Reagent Type Coomassie Blot Type Western blot Detection Technique Colorimetry
From $ 88.00 (1000 ml)
Sizes 1 (1000 ml)
Catalog IDs 1610803
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Coomassie Brilliant Blue R-250 Staining Solutions Kit Bio-Rad
Reagent Type Coomassie Blot Type Western blot Detection Technique Colorimetry
From $ 140.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs 1610435
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Ponceau S solution Santa Cruz Biotechnology
Reagent Type Ponceau S Blot Type Western blot Detection Technique Colorimetry
From $ 78.00 (1000 ml)
Sizes 1 (1000 ml)
Catalog IDs sc-301558
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Western Blotting

Western blotting separates proteins on a gel by electrophoresis before transferring them to a membrane, upon which the proteins are detected by chemiluminescence, fluorescence or colorimetry.

See how Western Blotting is being used in breakthrough research in our article: Are Antibody Vaccines the answer to Alzheimer's Disease?

Southern Blotting

Southern Blotting uses electrophoresis to separate DNA fragments which are transferred to a positive nylon membrane.

Northern Blotting

Northern Blotting separates RNA fragments via electrophoresis before transferring the resultant bands to a positively charged nylon membrane.

Blotting Reagents Products

Blotting Reagents for these processes include:

Transfer Buffers
Transfer buffers help shift the sample over from the gel used in electrophoresis to the detection membrane.

Wash and Blocking Buffers
Wash buffers are repeatedly used during the blotting process. For example, in western blotting, between antibody incubation steps, wash buffers are used to remove any excess antibodies between primary and secondary antibody incubation steps. During northern and southern blotting, washing buffers remove unhybridized probes.

Blocking buffers, on the other hand, prevent the antibodies from nonspecific binding.

Filter / Blotting Paper
The filter paper is necessary for use in the transfer sandwich to enable quick and efficient transfer by supporting wicking and uniform capillary action, as well as preventing contamination.

Blotting Membranes
The blotting membrane (used for detection) consists of either nitrocellulose or PVDF (polyvinylidene fluoride). Both membranes are suitable for use in Western Blots, while PVDF is also capable of binding nucleic acids well, so it can be used in northern, southern and dot blots as well. However, northern and southern blotting also use positively charged nylon membranes, which can permanently bind nucleic acids with low background and high target retention.

Detection Reagents
Detection reagents include chemiluminescent substrates, chromogenic substrates and fluorescence detection kits. All these reagents interact with a probe on the antibody bound to the protein of interest (western) or a hybridization probe (northern/southern) to provide a visual signal.

Chemiluminescent substrates have the greatest sensitivity, able to quantify concentration and can be used after reprobing. They require the use of an x-ray film and dark room to detect the light emission created by a reaction between two solutions.

Chromogenic substrates do not need any visualization equipment because they form an insoluble colored precipitate on contact with the appropriate enzyme. However, this method lacks sensitivity.

Fluorescent kits can quantitatively analyze a blot without a darkroom or film and can be used to target multiple, different samples on the same blot without reprobing.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.