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Gene Editing Controls Kits on ZAGENO

Good gene editing controls are a critical tool for troubleshooting and ensuring that the transfer worked according to plan, especially due to the high sensitivity of gene transfer experiments. RNA interference (RNAi) and reporter gene experiments assess the activity and effects of certain genes. These techniques require controls to test transfection efficiency, knockdown efficiency (RNAi), and whether conditions are still optimal (reporter genes).

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Gene Transfer Controls

Gene transfer introduces specific genetic sequences into a new host cell. This process tests whether overexpression of genetic material alters cellular functions, or if the expression of a particular gene is possible in a certain cellular system.

When choosing your gene transfer control, there are a few factors to consider. For instance, there is a difference in procedure between bacterial and mammalian cells.

When transforming bacteria, it is common to perform an additional transformation with a control vector. Vectors such as the pUC18 plasmid or the pUC19 vector are normally used for such cases. These cloning vectors, given their multiple cloning and restriction sites, can easily introduce foreign DNA into the plasmid. Antibiotics can be used as a control for transformed bacteria. This is done with a small plasmid which carries an appropriate antibiotic resistance. In addition to this, the X-gal system provides an extra layer of control by color-coding successfully transformed colonies that carry the right insert. One key example of this process is the blue-white screening technique, as seen with the pUC19 plasmid system.

Antiobiotics can also be used as controls for transformed mammalian cells. However, cells growing on antibiotics might display physiological differences that could influence your experiments. An alternative gene transfer control involves vectors which express a fluorescent protein alongside with the gene of interest. This can also be used for co-transfection of two vectors, using multiple fluorescent proteins, or for automatic selection of successful transfection.

Reporter Gene / siRNA Controls

Reporter control siRNAs ensure high reporter gene knockdown of genes such as GFP and luciferase, which indicate gene expression. Knockdown of these genes allows you to visualise when conditions are suboptimal and need to be changed; this is indicated by test samples fading to a similar level of the control.

RNAi controls are able to fully inhibit your RNA of choice or leave it completely unaffected, and therefore give a measure of the efficacy of your technique of inhibition.

Compare Positive Luciferase Knockdown Controls

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For instance, this comparison shows:

  • that the LUCIFERASE GL2 SIRNA and LUCIFERASE GL3 SIRNA kits from Qiagen are cheaper per nmol.
  • that the SILENCER® FIREFLY LUCIFERASE (GL2 + GL3) SIRNA Kit from Ambion can knockdown both GL2 & GL3 plasmids.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.


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