Gene Transfer on Zageno
Transformation - if the target is a non-animal cell.
Bacteria transformation is a staple technique in cell biology labs, used for cloning or protein expression. Transformation protocols rely on transforming bacterial cells into competent cells via chemical plasmid transformation or electroporation, with dedicated kits for both.
While chemical transformation is very cost-effective, electroporation can reach have high transformation efficiency. Keep in mind that electroporators can be used for other cells, such as plant cells, which might justify the investments.
Solve any experiement problems with our Transformation Troubleshoot.
Transfection & Transduction
Transfection - when a non-viral transfer method is used for genetic transfer.
Transduction - when a viral vector is used for genetic transfer.
The term “transfection” is mostly used for describing the insertion of nucleic acids into a cell by non-viral methods. The process is often carried out by electroporation, cell squeezing, or via chemical methods like calcium phosphate exposure that opens transient holes in cell membranes allowing target nucleic acids to enter into the cells. Sometimes liposomes are used to allow the membrane to be crossed without any permanent damage to the cell.
Perfect your experiement with our Transfection Troubleshoot.
For mammalian cells, the main factor to consider is cell type. There are different kits on the market optimizing the transfection or transduction process for specific cells types. Also, keep in mind that different kits can result in different expression levels. This is particularly important if your experimental set-up includes a phenotypical analysis with a range of expression levels of your protein, or if you expect a cytotoxic effect.
Master the technqiue with our Lentiviral Transduction Troubleshoot.
Moreover, if you want to transfer miRNA or siRNA instead of DNA, be sure to use a kit that specifically does that. In particular, it is possible to transduce purified siRNA with physical methods, without the use of vectors.
Compare Genomic DNA Transfer Kits
With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.
Click on the comparison below for a clearer view!
For example, this comparison shows:
- that the Mammalian Transfection Kit from Agilent also includes controls.
- that the Ecotransfect Kit from OZ Biosciences is the cheapest per reaction.
- that the DNAfectin 2100 Transfection Reagent requires as little as a day to complete its protocol
This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.
The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.
Check out our How It Works page for a guide to using the comparison function.