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In Vitro Transcription on Zageno

T7 High Yield In Vitro Transcription Kit MCLAB
Starting Material dsDNA Time to Sample 2-4 hrs Yield /
From $ 110.00 (20 Reactions)
Sizes 2 (20 - 100 Reactions)
Catalog IDs T7HT-100, T7HT-200
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T7 Transcription Kit Jena Bioscience
Starting Material dsDNA Time to Sample 45 mins to 2 hrs Yield /
From $ 74.00 (40 Reactions)
Sizes 2 (40 - 200 Reactions)
Catalog IDs PCR-601S, PCR-601L
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HiScribe™ T7 ARCA mRNA Kit New England Biolabs
Starting Material dsDNA Time to Sample > 45 min Yield 20 µg
From $ 307.00 (20 Reactions)
Sizes 1 (20 Reactions)
Catalog IDs E2065S
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EnGen® sgRNA Synthesis Kit, S. pyogenes New England Biolabs
Starting Material dsDNA Time to Sample 30 mins Yield 4-25 µg of sgRNA
From $ 400.00 (20 Reactions)
Sizes 1 (20 Reactions)
Catalog IDs E3322S
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AmpliCap-Max™ T7 High Yield Message Maker Kit CellScript
Starting Material dsDNA Time to Sample 50-60 µg Yield > 30 min
From $ 328.00 (25 Reactions)
Sizes 1 (25 Reactions)
Catalog IDs C-ACM04037
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AmpliScribe™ T7-Flash™ Transcription Kit Epicentre
Starting Material dsDNA, Plasmid DNA, cDNA Time to Sample 120 min Yield 150-180 µg
From $ 202.00 (25 Reactions)
Sizes 2 (25 - 50 Reactions)
Catalog IDs ASF3257, ASF3507
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MEGAscript® T7 Transcription Kit Invitrogen
Starting Material Plasmid DNA Time to Sample 24 h Yield 100 µg
From $ 217.00 (25 Reactions)
Sizes 3 (25 - 200 Reactions)
Catalog IDs AM1333, AM1334, AMB13345
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MAXIscript® T7/T3 Kit (50 Reactions each) with Manual Invitrogen
Starting Material Plasmid DNA Time to Sample 10 min Yield 2-6 µg
From $ 322.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs AM1326M
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MEGAscript® T3 Kit Invitrogen
Starting Material Plasmid DNA Time to Sample 120 min Yield 100 μg
From $ 319.00 (40 Reactions)
Sizes 1 (40 Reactions)
Catalog IDs AM1338
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pBIVT Vector Set - 1, 5 µg each Bioneer
Starting Material dsDNA, PCR products Time to Sample / Yield /
From $ 262.10 (5 µg)
Sizes 1 (5 µg)
Catalog IDs K-7350
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AmpliScribe™ T7-Flash™ Biotin-RNA Transcription Kit Epicentre
Starting Material dsDNA Time to Sample 120 min Yield 150-180 µg
From $ 392.00 (10 Reactions)
Sizes 1 (10 Reactions)
Catalog IDs ASB71110
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Silencer® siRNA Construction Kit Ambion
Starting Material ssDNA Time to Sample < 24 h Yield /
From $ 754.00 (15 Reactions)
Sizes 1 (15 Reactions)
Catalog IDs AM1620
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

The in vitro transcription process

The starting template of in vitro transcription is a linear DNA template that includes a promoter region together with the usual ingredients for a successful transcription process: RNA polymerase, nucleotides, and an appropriate buffer system. Since every reaction is different, the conditions may vary depending on your DNA template, expected RNA yield, length, and purity.

Make use of our In Vitro Transcription Troubleshoot for any experimental issues.

Templates used

You need a double stranded DNA (dsDNA) template, but its origin is irrelevant. The template can be anything from normal PCR reactions, plasmids, or even cDNA templates that themselves were generated from RNA molecules. However, different kits may be suitable for different templates, so take care when choosing your kit.

  • In order to use a PCR product in an in vitro transcription reaction, you have to add a promoter to your fragment so that it is recognized by RNA polymerase. The promoter is added at the 5’ end of the fragment.
  • Plasmid vectors must first be linearized before the transcription reaction can ensue. This is (fairly) easily done by using restriction enzymes. Purification should be carried out after such a digest as the restriction enzymes may inhibit the downstream in vitro transcription process.
  • One of the latest developments in this field is using in vitro transcription in RNA amplification reactions. Here, the reaction template is created from RNA during reverse transcription, generating cDNA. A second strand synthesis reaction is then performed to convert the cDNA to dsDNA for the in vitro transcription to be carried out.

Other applications also exists such as the use of oligonucleotides as templates.

Compare In Vitro Transcription Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the SP6/T7 TRANSCRIPTION Kit from Roche Life Science is suitable for downstream in vitro and in vivo translation.
  • that the MEGASCRIPT® SP6 TRANSCRIPTION Kit from Invitrogen is the cheapest per reaction.
  • that the RIBOPROBE® COMBINATION SYSTEM-SP6/T7 RNA POLYMERASE Kit only requires an hour to produce the final sample.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.