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Spectrophotometry Equipment on ZAGENO

Spectrophotometer Cuvettes, 1.5ml Globe Scientific
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From $ 68.00 (400 Cuvettes)
Sizes 1 (400 Cuvettes)
Catalog IDs 112137
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CrystalCap SPINE HT Hampton Research
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From $ 237.00 (30 Items)
Sizes 1 (30 Items)
Catalog IDs HR8-132
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18 mm Mounted CryoLoop - 20 micron Hampton Research
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From $ 66.50 (25 Items)
Sizes 1 (25 Items)
Catalog IDs HR4-974
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22 mm x 0.22 mm Siliconized circle cover slides Hampton Research
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From $ 29.80 (120 Slides)
Sizes 1 (120 Slides)
Catalog IDs HR3-231
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BRAND® UV cuvette micro center H 8.5 mm, volume 70-850 μL BRAND
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From $ 84.00 (100 Cuvettes)
Sizes 2 (100 Cuvettes)
Catalog IDs BR759200-100EA, BR759215-100EA
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CrystalCap SPINE HT Hampton Research
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From $ 237.00 (30 Items)
Sizes 1 (30 Items)
Catalog IDs HR8-120
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Fluorimeter cuvettes Polystyrene, without stopper Sigma-Aldrich
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From $ 23.70 (100 Cuvettes)
Sizes 1 (100 Cuvettes)
Catalog IDs C0918-100EA
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Biacore Maintenance Kit, type 2 GE Healthcare
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From $ 259.00 (1 Kits)
Sizes 1 (1 Kits)
Catalog IDs BR100651
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CrystalCap SPINE HT Hampton Research
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From $ 237.00 (30 Items)
Sizes 1 (30 Items)
Catalog IDs HR8-124
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CrystalCap SPINE HT Hampton Research
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From $ 237.00 (30 Items)
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Catalog IDs HR8-126
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Fisherbrand™ Disposable Cuvettes Fisherbrand
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From $ 154.70 (500 Cuvettes)
Sizes 1 (500 Cuvettes)
Catalog IDs 14-955-127
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CryoCane 5 Vial Holder Hampton Research
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From $ 20.20 (12 Items)
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Catalog IDs HR4-709
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Spectrophotometry Procedure

Spectrophotometry uses visible light, near-infrared light, and near-ultraviolet light to measure the reflection and transmission properties of solutions and cultures. The light intensity measurement is then converted to a function from which researchers can determine concentration or purity of solutions, as well as cell density. The quantitative analysis provided can be applied to forensic examinations as well as clinical studies.

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Spectrophotometers

Spectrophotometers are the most important instrument required for spectrophotometry and can measure different frequencies transmitted or reflected:

• UV-Vis spectrophotometers (ultraviolet-visible) measure the UV (190 to 380 nm) and visible (380 to 760 nm) spectrums of light

• Visible spectrophotometers measure visible light (380 to 760 nm).

• UV-Vis near-infrared spectrophotometers (UV VIS NIR) measure near-infrared frequencies (2600 to 3300 nm).

Single beam spectrophotometers require a standard to set the blank measurement before reading the test solution. On the other hand, double beam spectrophotometers, split the beam of light so that one hits the standard and the other the test sample. The ratio of intensities is measured, averting discrepancies in the light source or detector. However, this variant is bulkier and has a lower dynamic range.

NanoDrops

NanoDrops™ are also classified as spectrophotometers; able to measure the concentration of DNA, RNA or protein in a single drop of solution.

Colorimeters

Colorimeters measure light absorbance at specific frequencies, i.e. different colors, from a colored sample. Also able to gauge the concentration of a solute, the transmitted wavelength must match the absorbance frequency of the sample. Applications include pharmaceutical analysis, environmental testing, and biochemistry.

Microplate Readers

Microplate readers (plate readers) use pre-defined protocols and standard filters to measure, absorbance, luminescence, and fluorescence from solutions in a 96-well plate or larger. They are particularly suited towards ELISA and protein quantitation.