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Spectrophotometry Equipment

Spectrophotometer Cuvettes, 1.5ml

Globe Scientific

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From
$ 68.00 (400 Cuvettes)
Sizes
1 (400 Cuvettes)
Catalog IDs
112137
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Spectrophotometry Equipment

CrystalCap SPINE HT

Hampton Research

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From
$ 237.00 (30 Items)
Sizes
1 (30 Items)
Catalog IDs
HR8-132
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Spectrophotometry Equipment

18 mm Mounted CryoLoop - 20 micron

Hampton Research

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From
$ 66.50 (25 Items)
Sizes
1 (25 Items)
Catalog IDs
HR4-974
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Spectrophotometry Equipment

22 mm x 0.22 mm Siliconized circle cover slides

Hampton Research

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From
$ 29.80 (120 Slides)
Sizes
1 (120 Slides)
Catalog IDs
HR3-231
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Spectrophotometry Equipment

Specac® variable pathlength cell for IR spectroscopy SKU : Z801348

Specac

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From
$ 3,073.00 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
Z801348-1EA
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Spectrophotometry Equipment

picoSpin™ 45 Series II NMR Spectrometers SKU : 143-773400

Thermo Scientific

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$ 416.98 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
143-773400
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Spectrophotometry Equipment

picoSpin™ 80 Series II NMR Spectrometers SKU : 143-773800

Thermo Scientific

From
$ 812.76 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
143-773800
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Spectrophotometry Equipment

SPECTRONIC™ 200 Spectrophotometer SKU : 222-265600

Thermo Scientific

From
$ 35.56 (6 Units)
Sizes
1 (6 Units)
Catalog IDs
222-265600
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Spectrophotometry Equipment

Rectangular Cells SKU : 268-799700

Thermo Scientific

From
$ 397.17 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
268-799700
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Spectrophotometry Equipment

Rectangular Cells SKU : 268-799800

Thermo Scientific

From
$ 412.51 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
268-799800
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Spectrophotometry Equipment

Accessories for the SPECTRONIC 20 and 20D SKU : 333385-000-10

Thermo Scientific

From
$ 323.60 (10 Units)
Sizes
1 (10 Units)
Catalog IDs
333385-000-10
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Spectrophotometry Equipment

GENESYS™ 10S Cell Holder, Single SKU : 336028-000

Thermo Scientific

From
$ 51.58 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
336028-000
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Spectrophotometry Procedure

Spectrophotometry uses visible light, near-infrared light, and near-ultraviolet light to measure the reflection and transmission properties of solutions and cultures. The light intensity measurement is then converted to a function from which researchers can determine concentration or purity of solutions, as well as cell density. The quantitative analysis provided can be applied to forensic examinations as well as clinical studies.

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Spectrophotometers

Spectrophotometers are the most important instrument required for spectrophotometry and can measure different frequencies transmitted or reflected:

• UV-Vis spectrophotometers (ultraviolet-visible) measure the UV (190 to 380 nm) and visible (380 to 760 nm) spectrums of light

• Visible spectrophotometers measure visible light (380 to 760 nm).

• UV-Vis near-infrared spectrophotometers (UV VIS NIR) measure near-infrared frequencies (2600 to 3300 nm).

Single beam spectrophotometers require a standard to set the blank measurement before reading the test solution. On the other hand, double beam spectrophotometers, split the beam of light so that one hits the standard and the other the test sample. The ratio of intensities is measured, averting discrepancies in the light source or detector. However, this variant is bulkier and has a lower dynamic range.

NanoDrops

NanoDrops™ are also classified as spectrophotometers; able to measure the concentration of DNA, RNA or protein in a single drop of solution.

Colorimeters

Colorimeters measure light absorbance at specific frequencies, i.e. different colors, from a colored sample. Also able to gauge the concentration of a solute, the transmitted wavelength must match the absorbance frequency of the sample. Applications include pharmaceutical analysis, environmental testing, and biochemistry.

Microplate Readers

Microplate readers (plate readers) use pre-defined protocols and standard filters to measure, absorbance, luminescence, and fluorescence from solutions in a 96-well plate or larger. They are particularly suited towards ELISA and protein quantitation.