Microcentrifuge Tube Applications
Microcentrifuge tubes – often referred to as Eppis (short for Eppendorf reaction tubes) – are a common staple of lab plasticware, that can be used for various applications including:
• Preparation of reaction mixtures
• Storage of samples like RNA, cDNA, protein lysates, and cells
• Used to aliquot stock solutions like antibodies and different reagents
• Centrifugation of small volumes e.g. cell suspensions or beads resulting in pellets and supernatants
• Incubation of various reactions at numerous temperatures e.g. restriction endonuclease reactions or ligation reactions
Microcentrifuge Tube Variations
In most cases, tubes are made of polypropylene, but different materials are available too. They need to withstand high centrifugal force as well as a wide range of temperatures. Microcentrifuge tubes are also resistant to commonly used chemicals like acids and alkaline solutions.
Tubes can also come pre-sterilized (by radiation) and are often autoclavable.
Microcentrifuge tubes are suitable for volumes in the microliter (µl) range and also up to 5 ml. They vary between round, conical or flat bottoms and are most commonly transparent. However colored tubes are available to help you identify and sort your samples. For reagents or samples sensitive to light use black or brown tubes, which are optically opaque. Screw and SafeSeal caps are suited for storage or procedures where a tight-fitting cap is required.
For the storage of cells in liquid nitrogen tanks, you should choose distinct cryovials designed for extreme negative temperatures. They are equipped with a screw cap and a silicone washer seal to ensure cell safety.