PCR Tube Functionalities
PCR tubes are small plastic tubes able to withstand high temperatures and which fit perfectly into a thermocycler. The tubes are adapted to quickly distribute the temperature from the cycler to the reaction mix, with caps which seal the tube tightly. UTW tubes offer thinner walls for minimum thermal resistance and lower incubation times.
Tubes are often made of polypropylene and may be autoclavable, while DNase- and RNase-free options also exist.
cDNA synthesis is often carried out in thermocyclers also. Therefore PCR tubes are also suitable for reverse transcription reactions.
PCR Tube Options
Depending on your needs, you have the choice between single tubes and strips of tubes. The most common length is 8-tube/8-well tube strips, but other sizes are also available. Strips of caps or tubes without caps are also provided separately.
The most common size for PCR tubes is 0.2 mL, but other sizes are available too. Low-profile tubes are particularly suited for low reaction volumes, including below 20µl. Colored tubes are also available to help distinguish your probes from one another.
Depending on your thermocycler and your needs you have the choice between domed caps or flat caps. To prevent evaporation of the reaction mix the heated lid of the instrument has to be in direct contact with the cap. In some cyclers there remains a small space between the lid and a flat cap; the domed cap reduces this ‘dead space’ to prevent condensation on the tube walls and loss of efficiency.