ZAGENO
Results: 1-12 of 100+ products
Sort by:
Show as:

PCR (Polymerase Chain Reaction) on Zageno

SNORD48 primer set Exiqon
Reagent Type / Applicable Processes / Quantity /
From $ 144.44 (200 Reactions)
Sizes 1 (200 Reactions)
Catalog IDs 203903
Added to comparison view - remove
View Product
7500 Real Time PCR Systems Spectral Calibration Kit I Thermo Fisher Scientific
Reagent Type Calibration standard Applicable Processes Calibration Quantity 1 Kit
From $ 1,030.00 (1 Kit)
Sizes 1 (1 Kit)
Catalog IDs 4349180
Added to comparison view - remove
View Product
AccuPower GreenStar qPCR PreMix, For Bio-Rad Systems, 0.2 ml 8-tube strip w/ cap, 20 µl x 96 rxns, optical film included Bioneer
Fluorescent Dye Included Templates dsDNA, cDNA, Genomic DNA, Plasmid DNA, Viral DNA Amplicon Size < 200 bp
From $ 98.30 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-6212
Added to comparison view - remove
View Product
AccuPower Plus DualStar™ qPCR PreMix (with UDG), For Opticon Systems, 0.2 ml 8-tube strip w/ cap, 50 µl x 96 rxns, optical film included Bioneer
Fluorescent Dye Included Templates Genomic DNA, dsDNA, cDNA, Plasmid DNA, Viral DNA Amplicon Size < 200 bp
From $ 205.00 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-6607
Added to comparison view - remove
View Product
AccuPower PCR PreMix, 0.2 ml 8-tube strip w/ cap, 20 µl x 96 rxns Bioneer
Templates dsDNA, Genomic DNA, Plasmid DNA, Viral DNA, cDNA Fidelity No proofreading activity Amplicon Size < 10 kb
From $ 58.50 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-2012
Added to comparison view - remove
View Product
AccuPower RT-PCR PreMix, 1 x 96-well flat plate, 20 µl rxn Bioneer
Templates mRNA, Total RNA, Viral RNA Fidelity / Amplicon Size /
From $ 251.80 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-2262-4
Added to comparison view - remove
View Product
AccuPower RocketScript™ RT-PCR PreMix, 0.2 ml 8-tube strip w/ cap, 50 µl x 96 rxns Bioneer
Templates mRNA, Total RNA, Viral RNA Fidelity / Amplicon Size /
From $ 734.20 (96 Reactions)
Sizes 1 (96 Reactions)
Catalog IDs K-2503
Added to comparison view - remove
View Product
FastStart Universal SYBR Green Master (Rox) Roche Life Science
Fluorescent Dye Included Templates Plasmid DNA, Genomic DNA, cDNA Amplicon Size < 500 bp
From $ 321.00 (500 Reactions)
Sizes 2 (500 - 5000 Reactions)
Catalog IDs 04913850001, 04913914001
Added to comparison view - remove
View Product
Pfu DNA Polymerase MasterMix [2X] G-Biosciences
Templates Plasmid DNA, Genomic DNA, Viral DNA Fidelity Proofreading activity Amplicon Size /
From $ 66.00 (40 Reactions)
Sizes 1 (40 Reactions)
Catalog IDs 786-817
Added to comparison view - remove
View Product
QuantiFast Multiplex RT-PCR Kits QIAGEN
Fluorescent Dye Not included Templates Total RNA, mRNA Amplicon Size 60-150 bp
From $ 208.00 (80 Reactions)
Sizes 5 (80 - 2000 Reactions)
Catalog IDs 204852, 204952, 204854, ...
Added to comparison view - remove
View Product
Maxima Probe/ROX qPCR Master Mix (2X) Thermo Scientific
Fluorescent Dye Not included Templates Plasmid DNA, Genomic DNA, cDNA, Viral DNA Amplicon Size 70-150 bp
From $ 172.00 (200 Reactions)
Sizes 3 (200 - 4000 Reactions)
Catalog IDs K0231, K0232, K0233
Added to comparison view - remove
View Product
2X One-Step RT-PCR Master Mix with Dual Dye Norgen Biotek
Templates Total RNA, mRNA Fidelity No proofreading activity Amplicon Size < 1.5 kb
From $ 243.00 (100 Reactions)
Sizes 3 (100 - 500 Reactions)
Catalog IDs 28242, 28273, 28274
Added to comparison view - remove
View Product

PCR Amplification

The polymerase chain reaction (PCR) is an in vitro method used for the amplification of a specific DNA template to generate a large number of copies in the span of just a few hours. The development of the PCR method in 1983 by Kary Mullis replaced the old and slow cloning techniques which required days of work.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

PCR Kits and Methods

Real-Time PCR

Real-time PCR is a technique that monitors DNA amplification in real-time rather than at the end of the PCR run, as is the case for End-Point PCR.

End-Point PCR

PCR enables amplification of specific DNA fragments with the help of a thermocycler and is a widespread molecular biology technique.

PCR Reagents

PCR requires five reagents; DNA/RNA template, DNA polymerase, primers (forward and reverse), dNTPs and PCR buffers.

The benefit of PCR

Another benefit of the PCR method is its high sensitivity; theoretically, a single copy of the template is enough to start the reaction. The sensitivity and speed make the PCR techniques useful in both basic research and for commercial applications like in vitro diagnostics, forensics, and quality control.

The basic principles of the PCR method

To start a PCR reaction the following is needed:

  • A template: a DNA or RNA strand that we want to amplify.
  • Nucleotides (dCTP, dGTP, dATP, dGTP): the building blocks from which DNA is made.Primers: short nucleotide strands that will anneal to the position that we want to start and end the amplification.
  • DNA polymerase: the enzyme catalyzing the reaction.
  • (Accessory additives like buffers and Mg2+)

Our cDNA synthesis Troubleshoot will guide you though any initial setbacks.

The amplification process:

  • Denaturation: the DNA molecule is split into two single strands of DNA by heating (carried out in a PCR thermocycler)
  • Hybridization: During a cool-down phase, the primers anneal to the target sequences of the single stranded DNA
  • Elongation: Polymerase extends the DNA molecule starting at the primer sequences, and so doubles the template numbers
  • The process is repeated from Step 1 achieving an exponential increase in the number of templates until the substrate runs out or the product accumulation is halting the reaction.

Check out our End-Point PCR Troubleshoot if you encounter any problems.

The past, present and future of PCR

PCR was originally developed to detect specific DNA sequences in a sample, and though this is still the most used application, many other applications have been developed over the past decades.
Nowadays there exist different types of PCRs e.g. End-Point PCR, qPCR (quantitative Real-time PCR), RT-PCR (Reverse Transcription PCR), Multiplex PCR, Nested PCR and Digital PCR. Depending on the polymerases used, the PCR is featured with special properties like a hot start mechanism or proofreading activity to enable the best results.

See our Real-Time PCR Troubleshoot as well as the other troubleshooting guides for tips to perfect your experiment.

Video and Image Credits:

Video: DNA Learning Center/YouTube