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cDNA Synthesis on Zageno

MessageBOOSTER™ cDNA Synthesis from Cell Lysates Kit Epicentre
Templates Cells Template Amount 1-1000 cells Time to Sample < 9h 30 min
From $ 220.00 (10 Reactions)
Sizes 1 (10 Reactions)
Catalog IDs MBCL90310
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MMLV Reverse Transcriptase 1st-Strand cDNA Synthesis Kit Epicentre
Templates Total RNA, mRNA Template Amount < 1 µg total RNA Time to Sample > 70 min
From $ 216.00 (50 Reactions)
Sizes 1 (50 Reactions)
Catalog IDs MM070150
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5X All-In-One RT MasterMix (with AccuRT Genomic DNA Removal Kit) Applied Biological Materials
Templates mRNA, Total RNA Template Amount > 2 µg Time to Sample 32 min for qPCR, 70 min for PCR
From $ 160.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs G492
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Second-Strand cDNA Synthesis Kit-dNTP/dUTP based Applied Biological Materials
Templates cDNA Template Amount 10 ng - 2 µg first strand cDNA Time to Sample 150 min
From $ 455.00 (25 Reactions)
Sizes 1 (25 Reactions)
Catalog IDs G476
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RevertAid First Strand cDNA Synthesis Kit Thermo Scientific
Templates / Template Amount / Time to Sample /
From (on request)
Sizes 1 (100 Reactions)
Catalog IDs K1622
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Maxima H Minus First Strand cDNA Synthesis Kit Thermo Scientific
Templates / Template Amount / Time to Sample /
From (on request)
Sizes 1 (100 Reactions)
Catalog IDs K1652
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iScript™ Advanced cDNA Synthesis Kit for RT-qPCR Bio-Rad
Templates Total RNA, mRNA Template Amount 100 fg - 7.5 µg Time to Sample 21 min
From $ 190.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs 1725037, 1725038
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RT² PreAMP cDNA Synthesis Kit QIAGEN
Templates Total RNA Template Amount 1-100 ng total RNA from fresh/frozen samples; 100 ng - 1 µg total RNA from FFPE samples Time to Sample > 45 min (cDNA synthesis)
From $ 506.00 (12 Reactions)
Sizes 1 (12 Reactions)
Catalog IDs 330451
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SuperScript® II Reverse Transcriptase Invitrogen
Templates mRNA, Total RNA Template Amount 1 ng–5 μg total RNA, 1–500 ng mRNA Time to Sample 75 min
From $ 91.25 (2000 Units)
Sizes 3 (2000 - 40000 Units)
Catalog IDs 18064022, 18064014, 18064071
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HotScriptase RT Polymerase Genaxxon Bioscience
Templates Total RNA, mRNA Template Amount Recommended final template concentration: 5-500 ng/µl Time to Sample 27-62 min
From $ 99.00 (250 Units)
Sizes 2 (250 - 1000 Units)
Catalog IDs M3056.0250, M3056.1000
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HotScriptase RT Master Mix Probe without ROX Genaxxon Bioscience
Templates Total RNA, mRNA Template Amount Recommended final template concentration: 5-500 ng/µl Time to Sample 27-62 min
From $ 165.00 (1.25 ml)
Sizes 2 (1.25 - 6.25 ml)
Catalog IDs M3064.0050, M3064.0250
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miRNA cDNA Synthesis Kit Applied Biological Materials
Templates ssDNA, Total RNA Template Amount 2 μg total RNA, 200 ng small RNA Time to Sample 30-45 min
From $ 90.00 (25 Reactions)
Sizes 2 (25 - 100 Reactions)
Catalog IDs G269, G270
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Synthesizing cDNA

For research purposes, cDNA is typically synthesized from purified mRNA using an in-vitro reverse transcriptase reaction. There are plenty of kits available on the market, called either cDNA synthesis or reverse transcription kits, supplying all the reagents needed to make your cDNA synthesis easy and cost-effective. However, there are a few factors to consider when choosing the right product.
The starting templates are generally:

Typically, cDNA synthesis kits synthesize either first-strand or double-stranded cDNA:

  • Double-stranded synthesis is required for cloning applications

It is also essential to be aware of the sensitivity of your kit, particularly for quantitative applications. Kits able to amplify minute differences in mRNA expression can be the key to successful experiments. Similarly, thermostable enzymes allow for the use of higher temperatures that can disentangle complex RNA secondary structures, resulting in a more representative amplification and more precise quantification.
Last but not least, the kit should fit your experimental workflow. In this regards, sample processing speed might be worth considering, alongside with suitability for automatic processing or buffer compatibility with your downstream applications.

Check out our cDNA Synthesis Troubleshoot for further guidance.

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

Video Credits

Video: abhchannel/YouTube