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Competent Cells on Zageno

TransforMax™ EC100D™ pir+ Electrocompetent E.coli Epicentre
Competence Electro Antibiotic Resistance Streptomycin Bacterial Strain /
From $ 220.00 (500 µl)
Sizes 1 (500 µl)
Catalog IDs ECP09500
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TransforMax™ EPI300™ Chemically Competent E. coli Epicentre
Competence Chemical Antibiotic Resistance Streptomycin Bacterial Strain /
From $ 194.00 (10 Reactions)
Sizes 1 (10 Reactions)
Catalog IDs C300C105
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Acella™ Chemically Competent Cells MoBiTec
Competence Chemical Antibiotic Resistance No resistance Bacterial Strain BL21
From (on request)
Sizes 1 (600 µl)
Catalog IDs 36795
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TransforMax™ EC100™ Chemically Competent E. coli Epicentre
Competence Chemical Antibiotic Resistance Streptomycin Bacterial Strain /
From $ 130.00 (500 µl)
Sizes 1 (500 µl)
Catalog IDs CC02810
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MAX Efficiency® Stbl2™ Competent Cells Invitrogen
Competence / Antibiotic Resistance / Bacterial Strain /
From $ 269.00 (1000 µl)
Sizes 1 (1000 µl)
Catalog IDs 10268019
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Competent Cells, Bronze Efficiency Bioline
Competence Chemical Antibiotic Resistance No resistance Bacterial Strain /
From $ 78.00 (2 ml)
Sizes 1 (2 ml)
Catalog IDs BIO-85025
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One Shot® TOP10 Chemically Competent E. coli Invitrogen
Competence Chemical Antibiotic Resistance Streptomycin Bacterial Strain TOP10
From $ 248.00 (550 µl)
Sizes 3 (550 - 2100 µl)
Catalog IDs C404010, C404003, C404006
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TOP10 Electrocomp™ Kit Invitrogen
Competence Electro Antibiotic Resistance No resistance Bacterial Strain TOP10
From $ 244.00 (500 µl)
Sizes 3 (500 - 3000 µl)
Catalog IDs C66455, C66411, C66424
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BL21(DE3) Chemically Competent Cells Sigma-Aldrich
Competence Chemical Antibiotic Resistance No resistance Bacterial Strain BL21
From $ 43.70 (160 µl)
Sizes 5 (160 - 3200 µl)
Catalog IDs CMC0014-4X40UL, CMC0014-20X40UL, CMC0014-20X80UL, ...
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Mav203 Comp Yeast Cell Lib Scl Thermo Fisher Scientific
Competence / Antibiotic Resistance / Bacterial Strain /
From (on request)
Sizes 1 (1 Product)
Catalog IDs 11281011
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Electromax Dh10B Comp Cells Thermo Fisher Scientific
Competence / Antibiotic Resistance / Bacterial Strain /
From (on request)
Sizes 1 (1 Product)
Catalog IDs 18290015
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Max Eff Dh10Bac Comp Cells Thermo Fisher Scientific
Competence / Antibiotic Resistance / Bacterial Strain /
From (on request)
Sizes 1 (1 Product)
Catalog IDs 10361012
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Competent Cells

Thus, competent cells are typically E. coli cells with high competency values. Such values though are normally achieved artificially in the lab, via chemical or electrical means.

Chemically Competent Cells

Chemically competent cells are prepared via a solution containing CaCl₂ and other salts around 0 ⁰C. The Ca 2+ ions in solution help create pores within the cell membrane, allowing more entryways for foreign DNA to be taken up.

Electrocompetent Cells

Electrocompetent cells are prepared via electroporation. This requires a specific device which sends a pulse of electricity across the cells, which in theory disrupt the membrane, allowing the foreign DNA to pass through.

Like most things, one procedure is not necessarily better than the other; rather it mostly depends on your laboratory setup. Preparing electrocompetent cells is usually more efficient, yet require expensive equipment. Preparing chemically competent cells is cheaper and does not require special equipment, yet is less efficient.

Competent Cell Types

The E. coli strains which are optimized for cloning typically have recA and endA markers, which increase plasmid DNA quality and stability. Furthermore, competent cells optimized for cloning often have screening techniques implemented into the exogenous DNA. The most common being blue / white screening.

Blue-white screening is a common technique to detect whether your cells have absorbed foreign DNA. DNA of interest containing a special sequence is ligated into a vector, which is then absorbed by your competent cells. These are then grown with X-gal. Those successfully transformed will produce white colonies; cells containing no exogenous DNA will appear blue.

E. coli strains optimized for cloning are not well suited for protein expression, so when looking for the right strain for your experiment, be sure to choose the one most appropriate. Competent cells for protein expression are typically engineered to withstand high protein levels, and can also have greater transcriptional control and protein folding.

Genotype Nomenclature

For anyone just beginning their competent cell experiments, genotype-nomenclature can seem extremely daunting, which is understandable when:

F- mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araΔ139 Δ(ara-leu)7697 galU galK rpsL (StrR) endA1 nupG tonA::Ptrc-ccdA

is the genotype. So, the following is a quick guide to help you start decoding the meaning behind the symbols used in naming genotypes.

Genes: designated by three letters in lower case (usually related to the phenotype or pathway). Different genes involved in the same pathway are separated via a capital letter after the assigned three letter name.

Ex: Mutations involving the synthesis of pyrimidine are so named pyr. One gene involved with the productions of an enzyme is named pyrC while another gene encoding for a different enzyme is pyrD.

Alleles: Every allele mutation is assigned a number. In order to prevent confusion and error, for every pyr mutation, a unique number is assigned and not reused.

Ex: pyrC19

Phenotype: when denoting the phenotype of a particular gene, the abbreviation is not italicized and has the first letter capitalized (DnaA – protein produced by the dnaA gene)

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.