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End-Point PCR on Zageno

2X One-Step RT-PCR Master Mix Norgen Biotek
Templates Total RNA Fidelity No proofreading activity Amplicon Size < 1.5 kb
From $ 243.00 (100 Reactions)
Sizes 3 (100 - 500 Reactions)
Catalog IDs 28113, 28114, 28115
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Tth Polymerase Bioron
Templates Total RNA Fidelity No proofreading activity Amplicon Size > 2-3 kb
From $ 126.00 (500 Units)
Sizes 2 (500 - 2500 Units)
Catalog IDs 104005, 104025
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Taq Master Mix Bioron
Templates Genomic DNA Fidelity No proofreading activity Amplicon Size > 5 kb
From $ 55.00 (200 Reactions)
Sizes 2 (200 - 1000 Reactions)
Catalog IDs 101605, 101625
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2X PCR TaqFast MasterMix Applied Biological Materials
Templates cDNA, Genomic DNA, Plasmid DNA Fidelity No proofreading activity Amplicon Size < 12 kb
From $ 90.00 (200 Reactions)
Sizes 2 (200 Reactions)
Catalog IDs G280, G280-dye
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Tissue Ex-Amp PCR Kit (with Kodaq MasterMix) Applied Biological Materials
Templates Genomic DNA Fidelity Proofreading activity Amplicon Size /
From $ 85.00 (120 Reactions)
Sizes 1 (120 Reactions)
Catalog IDs G927
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DirectTissue PCR 2X Master Mix Genaxxon Bioscience
Templates Genomic DNA Fidelity No proofreading activity Amplicon Size /
From $ 66.00 (1 ml)
Sizes 2 (1 - 5 ml)
Catalog IDs 3005S, 3005M
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Pfu DNA Polymerase MasterMix [2X] G-Biosciences
Templates Plasmid DNA, Genomic DNA, Viral DNA Fidelity Proofreading activity Amplicon Size /
From $ 66.00 (40 Reactions)
Sizes 1 (40 Reactions)
Catalog IDs 786-817
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Phusion™ Flash High-Fidelity PCR Master Mix Thermo Scientific
Templates / Fidelity No proofreading activity Amplicon Size /
From $ 84.75 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs F548S
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KAPA Taq ReadyMix PCR Kit KAPA
Templates Genomic DNA Fidelity Proofreading activity Amplicon Size 3.5 - 5 kb
From $ 132.00 (250 Reactions)
Sizes 2 (250 Reactions)
Catalog IDs K1006, KK1024
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Type-it Microsatellite PCR Kit QIAGEN
Templates Genomic DNA Fidelity No proofreading activity Amplicon Size < 1.5 kb
From $ 97.70 (70 Reactions)
Sizes 3 (70 - 2000 Reactions)
Catalog IDs 206241, 206243, 206246
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Phusion U Hot Start DNA Polymerase Thermo Scientific
Templates Plasmid DNA, Genomic DNA, cDNA Fidelity Proofreading activity Amplicon Size < 7.5 kb (genomic DNA), < 20 kb (phage DNA)
From $ 164.00 (100 Units)
Sizes 2 (100 - 500 Units)
Catalog IDs F-555S, F-555L
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Taq 5X Master Mix New England Biolabs
Templates Plasmid DNA, Genomic DNA, cDNA, Viral DNA Fidelity No proofreading activity Amplicon Size < 5 kb
From $ 140.00 (500 Reactions)
Sizes 1 (500 Reactions)
Catalog IDs M0285L
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Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

End-Point PCR

End-Point PCR is distinguishable from real-time PCR as it is not quantitative; the results are instead visualized on a gel once the run has finished.

There are of course a variety of kits available on the market that are providing all the necessary PCR reagents for a successful End-Point PCR reaction. When choosing a kit, there are a few things to consider when picking the one for your experiments:

  • The first is templates, as DNA and RNA require different procedures. For example, if you are performing Reverse-Transcription PCR (RT-PCR), an RNA template is required to produce complementary DNA (cDNA). Such RT-PCR procedures are often divided into one- or two-step PCRs.
  • Next, choosing the right polymerase can be crucial for a successful experimental outcome.

Polymerases may differ in their processivity. The processivity is defined as the number of bases they will read on a DNA strand before falling off the template. This is important for cloning of long fragments to avoid truncated amplicons.

Some polymerases can also have proofreading activity, like the famous Klenow polymerase, which ensures that amplified fragments are a high fidelity copy of the original. This is generally measured as average mistakes per bases amplified and should be kept as low as possible to avoid unexpected mutations particularly during cloning experiments. If high fidelity is not essential (like for confirming plasmid inserts), then Taq polymerase is a good, cost-effective alternative.

Hot start PCR compatible polymerases can also help you to get rid of unwanted non-specific amplification.

The polymerase processing speed is another factor to consider. High-speed polymerases can save you time when using PCR programs with a long primer extension cycles.

Complex applications such as multiplex PCR, where the amplification of multiple targets in the same reaction occurs, or the creation of labeled DNA probes using modified dNTPs, also have dedicated kits to make your experimentation easier.

Our End-Point PCR Troubleshoot will simplify the process further.

Compare Reverse Transcription End-Point PCR Kits

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the ENHANCED AVIAN HS RT-PCR Kit from Sigma-Aldrich can also be used for Two-step PCR.
  • that the TRANSCRIPTOR ONE-STEP RT-PCR Kit from Roche Life Science is the cheapest per reaction.
  • that the SUPERSCRIPT® ONE-STEP RT-PCR SYSTEM WITH PLATINUM® TAQ DNA POLYMERASE Kit only requires storage at -10°C.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video Credits

Video: Oxford Academic (Oxford University Press)/YouTube