ZAGENO
Results: 1-12 of 100+ products
Sort by:
Show as:

Real-time PCR (qPCR) on Zageno

GreenHot Master Mix Bioron
Fluorescent Dye Included Templates cDNA Amplicon Size /
From $ 96.00 (200 Reactions)
Sizes 2 (200 - 1000 Reactions)
Catalog IDs 119205, 119225
Added to comparison view - remove
View Product
EvaGreen Express 2X qPCR MasterMix-ROX Applied Biological Materials
Fluorescent Dye Included Templates cDNA, Genomic DNA, Plasmid DNA Amplicon Size < 150 bp
From $ 125.00 (500 Reactions)
Sizes 1 (500 Reactions)
Catalog IDs MasterMix-ER
Added to comparison view - remove
View Product
One-Step EvaGreen qRT-PCR-ROX Applied Biological Materials
Fluorescent Dye Included Templates mRNA, Total RNA Amplicon Size 100-250 bp
From $ 115.00 (100 Reactions)
Sizes 1 (100 Reactions)
Catalog IDs G471-R
Added to comparison view - remove
View Product
Maxima SYBR Green/Fluorescein qPCR Master Mix (2X) Thermo Scientific
Fluorescent Dye Included Templates Plasmid DNA, Genomic DNA, cDNA, Viral DNA Amplicon Size 70-150 bp
From $ 168.00 (200 Reactions)
Sizes 3 (200 - 4000 Reactions)
Catalog IDs K0241, K0242, K0243
Added to comparison view - remove
View Product
QuantiFast SYBR Green PCR Kit (2000) QIAGEN
Fluorescent Dye / Templates / Amplicon Size /
From (on request)
Sizes 1 (1 Product)
Catalog IDs 204056
Added to comparison view - remove
View Product
QuantiFast SYBR Green PCR Kit (4000) QIAGEN
Fluorescent Dye / Templates / Amplicon Size /
From (on request)
Sizes 1 (1 Product)
Catalog IDs 204057
Added to comparison view - remove
View Product
QuantiFast Pathogen PCR +IC Kit QIAGEN
Fluorescent Dye Not included Templates Plasmid DNA, Viral DNA Amplicon Size 60-150 bp
From $ 239.00 (100 Reactions)
Sizes 2 (100 - 400 Reactions)
Catalog IDs 211352, 211354
Added to comparison view - remove
View Product
TaqMan® PreAmp Master Mix Applied Biosystems
Fluorescent Dye Not included Templates cDNA Amplicon Size /
From $ 932.00 (40 Reactions)
Sizes 2 (40 - 200 Reactions)
Catalog IDs 4391128, 4488593
Added to comparison view - remove
View Product
Fast SYBR® Green Master Mix Applied Biosystems
Fluorescent Dye Included Templates Genomic DNA, cDNA Amplicon Size 50-150 bp
From $ 100.00 (100 Reactions)
Sizes 6 (100 - 5000 Reactions)
Catalog IDs 4385610, 4385612, 4385616, ...
Added to comparison view - remove
View Product
RT² SYBR Green Fluor FAST Mastermix QIAGEN
Fluorescent Dye Included Templates cDNA Amplicon Size /
From $ 196.00 (250 Reactions)
Sizes 5 (250 - 3000 Reactions)
Catalog IDs 330610, 330611, 330612, ...
Added to comparison view - remove
View Product
RT² SYBR Green Fluor qPCR Mastermix QIAGEN
Fluorescent Dye Included Templates cDNA Amplicon Size /
From $ 196.00 (200 Reactions)
Sizes 6 (200 - 2400 Reactions)
Catalog IDs 330510, 330514, 330511, ...
Added to comparison view - remove
View Product
EXPRESS qPCR Supermix, universal Invitrogen
Fluorescent Dye Not included Templates Plasmid DNA, Genomic DNA, cDNA Amplicon Size /
From $ 544.00 (500 Reactions)
Sizes 2 (500 - 2500 Reactions)
Catalog IDs 11785-200, 11785-01K
Added to comparison view - remove
View Product

Expand your understanding in our Knowledge Section. Perfect your experiments with our Troubleshooting Guides. Join in the discussion within our Community. Learn about ZAGENO at How It Works.

How does Real-Time PCR work?

Here at ZAGENO, you can find kits for different templates, such as:

Real-time PCR (qPCR) kits will provide polymerases, oligonucleotides, and buffers for your experiments. Before comparing kits from different suppliers on our product page, though, there are a few factors to consider:

  • You should keep in mind the size of your amplicon. While in general qPCR amplifies short fragments of around 150 bases, there are polymerases with higher processivity. A side note: if you plan on performing Hot Start qPCR (prevents non-specific amplification at room temperature), look for kits including enzymes suitable for this application.
  • If you expect to amplify a template present in low quantities in your sample, consider kits that use SYTO 9 as an intercalating dye, which has higher sensitivity. For standard applications, SYBR Green I is still an excellent choice.
  • Alternatively, probe-based qPCR, use labeled fluorescent probes instead of intercalates. In doing so provides excellent sensitivity for sequence–specific detection, but need to be prepared specifically for your template beforehand.
  • If you are using RT-qPCR, make sure to pick a one- or two-step kit, according to your experimental setup.

ZAGENO's Real-Time PCR Troubleshoot will help guide you through any diffculties encountered.

Compare Real-Time PCR Kits!

With our compare function, you can avoid all the time and energy wasted sifting through multiple web pages from different suppliers. At ZAGENO you can clearly see kits side-by-side, with the relevant attributes for each kit neatly in line for easy selection of the best product for you.

Click on the comparison below for a clearer view!

For example, this comparison shows:

  • that the TAKYON™ ONE-STEP ROX PROBE 5X MASTERMIX DTTP kit from Eurogentec is the cheapest per reaction.
  • that the ITAQ™ UNIVERSAL SYBR® GREEN ONE-STEP KIT from Bio-Rad includes a fluorescent dye.
  • that the TAQMAN® FAST VIRUS 1-STEP MASTER MIX from Applied Biosystems can also use Viral DNA as a template.

This comparison clearly exemplifies how every kit has strengths and weaknesses. Depending on what features you require for your experiment, these details should enable you to make an informed decision on the right kit for you.

The ZAGENO comparison does not highlight one kit to be better than the other, as the kit of choice may vary between researchers - depending on each individual's preferred attributes. The best kit is the one that meets your needs - ZAGENO allows you to make an informed decision with minimum effort.

Check out our How It Works page for a guide to using the comparison function.

Video Credits

Video: sccwrp/YouTube