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Columns & Beads

Score 8.86

Omnifit Labware (Diba) Columns, Glass: BENCHMARK COLUMN 10MM/150MM 2xA

Kinesis

From
$ 579.80 (1 Column)
Sizes
1 (1 Column)
Catalog IDs
006BCC-10-15-AA
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ZFN & TALEN

Score 0.10

CompoZr® Knockout ZFN Kit- CHO SLC35A1 SKU : ZFNSLC35A1

Sigma-Aldrich

Antigen
/
/
Host
/
/
Applicable Processes
/
/
From
$ 12,742.00 (1 Kit)
Sizes
1 (1 Kit)
Catalog IDs
ZFNSLC35A1-1KT
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Columns & Beads

Score 9.85

Glass beads SKU : Z250473

Sigma-Aldrich

From
$ 56.00 (357 Units)
Sizes
1 (357 Units)
Catalog IDs
Z250473-1PAK
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Columns & Beads

Score 7.92

Steri Sterilizer replacement glass beads SKU : Z380075

Sigma-Aldrich

From
$ 37.40 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
Z380075-1PAK
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Sample Cleanup Kits

Score 5.24

Vivaspin 500 centrifugal concentrators SKU : Z614009

Sartorius

Input Type
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/
Targets
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/
Removal
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/
From
$ 133.70 (25 Units)
Sizes
1 (25 Units)
Catalog IDs
Z614009-25EA
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Columns & Beads

Score 9.70

ACQUITY UPLC Protein BEH C4 VanGuard Pre-column, 300Å, 1.7 µm, 2.1 mm X 5 mm

Waters

From
$ 688.00 (3 Items)
Sizes
1 (3 Items)
Catalog IDs
186004623
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Columns & Beads

Score 4.36

Column tube assembly

GE Healthcare Life Sciences

From
$ 1,259.59 (1 Unit)
Sizes
1 (1 Unit)
Catalog IDs
28966649
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Resins

Score 8.61

Sephadex G-100 Superfine

Sephadex

Binding Capacity
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/
Composition
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/
Applicable Processes
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/
From
$ 629.00 (100 g)
Sizes
1 (100 g)
Catalog IDs
17006101
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Resins

Score 7.81

Sephadex G-75 Superfine

Sephadex

Binding Capacity
/
/
Composition
/
/
Applicable Processes
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/
From
$ 476.00 (100 g)
Sizes
1 (100 g)
Catalog IDs
17005101
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sgRNA, shRNA, siRNA & miRNA

Score 8.38

MISSION® siRNA SKU : SIHK1021

Sigma-Aldrich

From
$ 25.75 (0.25 nmol)
Sizes
1 (0.25 nmol)
Catalog IDs
SIHK1021-0.25NMOL
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sgRNA, shRNA, siRNA & miRNA

Score 8.37

MISSION® siRNA SKU : SIHK1026

Sigma-Aldrich

From
$ 25.75 (0.25 nmol)
Sizes
1 (0.25 nmol)
Catalog IDs
SIHK1026-0.25NMOL
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sgRNA, shRNA, siRNA & miRNA

Score 8.38

MISSION® siRNA SKU : SIHK1029

Sigma-Aldrich

From
$ 25.75 (0.25 nmol)
Sizes
1 (0.25 nmol)
Catalog IDs
SIHK1029-0.25NMOL
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Set-up of specialized techniques requires a certain input material or system; this is where sample preparation and gene editing are required, either to create a pure sample containing the target molecule or a genetic model with a particular behavior.

Preparation

Sample preparation is necessary for the majority of life science research experiments; enabling compatibility and accuracy while preventing interference from contaminants for successful analysis.

Initial steps include DNA or RNA Extraction (which can include the use of Columns & Beads), Protein Purification and Immunoprecipitation for isolation of the target molecule.

Sample cleanup and enrichment kits go one step further to increase concentration and remove any remaining impurities which may prevent proper analysis.

Sample preservation allows the prepared target molecule to be stabilized and stored until required; preventing degradation in the process.

Gene Editing

Genetic manipulation has recently been revolutionized by the discovery of CRISPR, allowing greater precision in gene editing than ever before.

Of course existing gene transfer techniques such as transfection, transformation, and transduction, are used to good effect. While Gene modification utilizes mutagenesis and bisulfite conversion to modify existing DNA, rather than introducing a new fragment. Gene silencing is slightly different in this respect, in that it negatively influences the expression of existing DNA via the use of short segments of DNA (shRNA, siRNA, and miRNA).

Gene editing controls offer a standardized model for comparison against the effects produced by the introduced genetic alteration. They, therefore, provide a test of reliability and accurate evaluation.

In vitro transcription produces a required RNA sample from existing DNA, useful for gene expression studies.