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Preparation & Gene Editing on ZAGENO

XK 50/30 Column GE Healthcare
Columns or Beads / Targets / Size Range /
From $ 1,125.00 (1 column)
Sizes 1 (1 column)
Catalog IDs 28988953
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CHOZN® DHFR-/- ZFN-Modified CHO Cell Line Sigma-Aldrich
Reagent Type ZFN & talen Applicable Processes / Quantity /
From $ 12,834.00 (1 VL)
Sizes 1 (1 VL)
Catalog IDs CHODHFR-1VL
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CompoZr® Knockout ZFN Kit- CHO DHFR Sigma-Aldrich
Reagent Type ZFN & talen Applicable Processes / Quantity /
From $ 12,834.00 (50 µg)
Sizes 1 (50 µg)
Catalog IDs ZFNDHFR-50UG
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Positive Control siRNA - Human GAPDH, 5 nmoles, Bio-RP Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 5 nmoles
From $ 85.10 (5 nmol)
Sizes 1 (5 nmol)
Catalog IDs SP-1001
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Positive Control siRNA - GFP, 20 nmoles, Bio-RP Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 20 nmoles
From $ 212.10 (20 nmol)
Sizes 1 (20 nmol)
Catalog IDs SP-2003
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Positive Control siRNA - GFP, 5 nmoles, HPLC Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 5 nmoles
From $ 151.20 (5 nmol)
Sizes 1 (5 nmol)
Catalog IDs SP-2011
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Positive Control siRNA - Luciferase, 20 nmoles, HPLC Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 20 nmoles
From $ 311.90 (20 nmol)
Sizes 1 (20 nmol)
Catalog IDs SP-3013
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Positive Control siRNA - Mouse Lamin A/C, 20 nmoles, Bio-RP Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 20 nmoles
From $ 212.10 (20 nmol)
Sizes 1 (20 nmol)
Catalog IDs SP-4003
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Negative Control siRNA, 20 nmoles, HPLC Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 20 nmoles
From $ 311.90 (20 nmol)
Sizes 1 (20 nmol)
Catalog IDs SN-1013
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Control siRNA Set - GAPDH, Positive 5 nmoles + Negative 2 nmoles, Bio-RP Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 5 nmoles (PC) + 2 nmoles (NC)
From $ 156.50 (1 Set)
Sizes 1 (1 Set)
Catalog IDs SS-1001
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Control siRNA Set - GAPDH, Positive 5 nmoles + Negative 2 nmoles, HPLC Bioneer
Reagent Type Small RNAs Applicable Processes / Quantity 5 nmoles (PC) + 2 nmoles (NC)
From $ 188.00 (1 Set)
Sizes 1 (1 Set)
Catalog IDs SS-1011
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Glass beads, acid-washed 425-600 µm (30-40 U.S. sieve) MilliporeSigma
Columns or Beads / Targets / Size Range /
From $ 124.66 (100 g)
Sizes 1 (100 g)
Catalog IDs G8772-100G
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Set-up of specialized techniques requires a certain input material or system; this is where sample preparation and gene editing are required, either to create a pure sample containing the target molecule or a genetic model with a particular behavior.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Preparation

Sample preparation is necessary for the majority of life science research experiments; enabling compatibility and accuracy while preventing interference from contaminants for successful analysis.

Initial steps include DNA or RNA Extraction (which can include the use of Columns & Beads), Protein Purification and Immunoprecipitation for isolation of the target molecule.

Sample cleanup and enrichment kits go one step further to increase concentration and remove any remaining impurities which may prevent proper analysis.

Sample preservation allows the prepared target molecule to be stabilized and stored until required; preventing degradation in the process.

Gene Editing

Genetic manipulation has recently been revolutionized by the discovery of CRISPR, allowing greater precision in gene editing than ever before.

Of course existing gene transfer techniques such as transfection, transformation, and transduction, are used to good effect. While Gene modification utilizes mutagenesis and bisulfite conversion to modify existing DNA, rather than introducing a new fragment. Gene silencing is slightly different in this respect, in that it negatively influences the expression of existing DNA via the use of short segments of DNA (shRNA, siRNA, and miRNA).

Gene editing controls offer a standardized model for comparison against the effects produced by the introduced genetic alteration. They, therefore, provide a test of reliability and accurate evaluation.

In vitro transcription produces a required RNA sample from existing DNA, useful for gene expression studies.