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Preparation & Gene Editing on ZAGENO

Aurum Affi-Gel Blue Mini Kit Bio-Rad
Input Type Other sources Targets Proteins Time to Sample 30 min
From $ 82.00 (2 Reactions)
Sizes 1 (2 Reactions)
Catalog IDs 7326712
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ZR Soil Microbe DNA MicroPrep™ Zymo Research
Extraction of Bacterial DNA, Genomic DNA, Mitochondrial DNA, Viral DNA Sample Type Cells, Environmental, Soil Time to Sample 15 min
From $ 216.00 (50 Preparations)
Sizes 1 (50 Preparations)
Catalog IDs D6003
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CHROMA SPIN™+TE-10 Columns Clontech
Columns or Beads Columns Targets Plasmid DNA, Total RNA Size Range > 10 bp
From $ 437.00 (50 Columns)
Sizes 1 (50 Columns)
Catalog IDs 636066
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ZR Genomic DNA™-Tissue MicroPrep Zymo Research
Extraction of Genomic DNA, Viral DNA, Bacterial DNA, Mitochondrial DNA Sample Type Blood, Cells, FFPE tissue, Plasma & serum, Tissue Time to Sample 10 min
From $ 119.00 (50 Preparations)
Sizes 2 (50 - 200 Preparations)
Catalog IDs D3040, D3041
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CHROMA SPIN™-30+DEPC-H2O Columns Clontech
Columns or Beads Columns Targets Genomic DNA, Total RNA Size Range 30 bp
From $ 467.00 (50 Columns)
Sizes 1 (50 Columns)
Catalog IDs 636087
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CHROMA SPIN™+STE-30 Columns Clontech
Columns or Beads Columns Targets Genomic DNA, Total RNA Size Range 30 bp
From $ 437.00 (50 Columns)
Sizes 1 (50 Columns)
Catalog IDs 636058
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CHROMA SPIN™+TE-200 Columns Clontech
Columns or Beads Columns Targets Genomic DNA, Total RNA Size Range /
From $ 437.00 (50 Columns)
Sizes 1 (50 Columns)
Catalog IDs 636082
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CalPhos™ Mammalian Transfection Kit Clontech
Type of Transfer Transfection Transfer of Plasmid DNA Cell Type Cell line
From $ 229.00 (700 Reactions)
Sizes 1 (700 Reactions)
Catalog IDs 631312
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MirTrap System Clontech
RNAi Type miRNA Targets mRNA Organism Animals
From $ 1,611.00 (15 Assays)
Sizes 1 (15 Assays)
Catalog IDs 632016
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Transformer™ Site-Directed Mutagenesis Kit Clontech
Organism Bacteria Efficiency >70–90% Time to Sample /
From $ 473.00 (30 Reactions)
Sizes 1 (30 Reactions)
Catalog IDs 630702
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Diversify™ PCR Random Mutagenesis Kit Clontech
Organism / Efficiency / Time to Sample >40 min
From $ 640.00 (30 Reactions)
Sizes 1 (30 Reactions)
Catalog IDs 630703
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CHROMA SPIN™-200+DEPC-H2O Columns Clontech
Columns or Beads Columns Targets Genomic DNA, Total RNA Size Range > 200 bp
From $ 260.00 (20 Columns)
Sizes 1 (20 Columns)
Catalog IDs 636096
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Set-up of specialized techniques requires a certain input material or system; this is where sample preparation and gene editing are required, either to create a pure sample containing the target molecule or a genetic model with a particular behavior.

Problems in the lab? Find the solutions in our Knowledge and Troubleshooting sections. Alternatively, our Community of experienced researchers can help! Still confused? See How ZAGENO Works.

Preparation

Sample preparation is necessary for the majority of life science research experiments; enabling compatibility and accuracy while preventing interference from contaminants for successful analysis.

Initial steps include DNA or RNA Extraction (which can include the use of Columns & Beads), Protein Purification and Immunoprecipitation for isolation of the target molecule.

Sample cleanup and enrichment kits go one step further to increase concentration and remove any remaining impurities which may prevent proper analysis.

Sample preservation allows the prepared target molecule to be stabilized and stored until required; preventing degradation in the process.

Gene Editing

Genetic manipulation has recently been revolutionized by the discovery of CRISPR, allowing greater precision in gene editing than ever before.

Of course existing gene transfer techniques such as transfection, transformation, and transduction, are used to good effect. While Gene modification utilizes mutagenesis and bisulfite conversion to modify existing DNA, rather than introducing a new fragment. Gene silencing is slightly different in this respect, in that it negatively influences the expression of existing DNA via the use of short segments of DNA (shRNA, siRNA, and miRNA).

Gene editing controls offer a standardized model for comparison against the effects produced by the introduced genetic alteration. They, therefore, provide a test of reliability and accurate evaluation.

In vitro transcription produces a required RNA sample from existing DNA, useful for gene expression studies.