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High Pure RNA Isolation Kit
Targets
Total RNA
Total RNA
Sample Type
Blood, Cells, Culture
Blood, Cells, Culture
Time to Sample
25 min
25 min
Scientific Score
9.94
Brand
Roche Life Science
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 259.00
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RNA/DNA/Protein Purification 96-Well Kit
Targets
Genomic DNA, Proteins, Total RNA, miRNA
Genomic DNA, Proteins, Total RNA, miRNA
Sample Type
Blood, Cells, Culture, Tissue
Blood, Cells, Culture, Tissue
Time to Sample
45 min
45 min
Scientific Score
8.07
Brand
Norgen Biotek
Sizes
1 (96 Reactions)
Available From
1 Supplier
Price
$ 703.00
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Plasma/Serum RNA Purification Mini Kit
Targets
Circulating RNA, Exosomal RNA, miRNA
Circulating RNA, Exosomal RNA, miRNA
Sample Type
Plasma & serum
Plasma & serum
Time to Sample
15-20 min
15-20 min
Scientific Score
7.22
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 345.00
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Sputum DNA Isolation Kit
Targets
Bacterial DNA, Genomic DNA
Bacterial DNA, Genomic DNA
Sample Type
Sputum
Sputum
Time to Sample
< 60 min
< 60 min
Scientific Score
7.24
Brand
Norgen Biotek
Sizes
1 (25 Reactions)
Available From
1 Supplier
Price
$ 142.00
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CRISPR-Lenti Human Positive Control DNA
Effect on Gene of Interest
Knock-out
Knock-out
Break Type
/
Target Organism
Human
Human
Scientific Score
10.00
Brand
Sigma-Aldrich
Sizes
1 (1 µg)
Available From
2 Suppliers
Price Range
$ 195.00 - $ 237.80
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FFPE RNA/DNA Purification Plus Kit
Targets
Genomic DNA, Total RNA, miRNA
Genomic DNA, Total RNA, miRNA
Sample Type
FFPE tissue
FFPE tissue
Time to Sample
70-160 min
70-160 min
Scientific Score
7.55
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 427.00
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GenElute™ mRNA Miniprep Kit
Starting Material
RNA
RNA
Final Product
mRNA
mRNA
Organism
Animals, Human
Animals, Human
Scientific Score
9.64
Brand
Sigma-Aldrich
Sizes
1 (10 Reactions)
Available From
2 Suppliers
Price Range
$ 149.15 - $ 159.00
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Food Oil DNA Isolation Kit
Targets
Genomic DNA
Genomic DNA
Sample Type
Food
Food
Time to Sample
25 min
25 min
Scientific Score
6.28
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 205.00
Catalog IDs
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GenElute™ RNA/DNA/Protein Purification Plus Kit
Targets
Genomic DNA, Proteins, Total RNA
Genomic DNA, Proteins, Total RNA
Sample Type
Blood, Cells, Culture, Tissue
Blood, Cells, Culture, Tissue
Time to Sample
< 30 min
< 30 min
Scientific Score
8.70
Brand
Sigma-Aldrich
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 615.00
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NucleoSpin® 8
Targets
Genomic DNA
Genomic DNA
Sample Type
Cells, Culture, Tissue
Cells, Culture, Tissue
Time to Sample
20 min
20 min
Scientific Score
9.27
Brand
Macherey-Nagel
Sizes
2 (96 - 480 Reactions)
Available From
1 Supplier
Price Range
$ 308.00 - $ 1,325.00
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High Pure PCR Cleanup Micro Kit
Input Type
Gel, PCR reaction
Gel, PCR reaction
Targets
Genomic DNA, cDNA
Genomic DNA, cDNA
Removal
Dimers, Salts, Small molecules, Unincorporated nucleotides, Unincorporated primers
Dimers, Salts, Small molecules, Unincorporated nucleotides, Unincorporated primers
Scientific Score
8.84
Brand
Roche Life Science
Sizes
2 (50 - 200 Reactions)
Available From
1 Supplier
Price Range
$ 120.00 - $ 412.00
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Plasma/Serum Exosome and Free-Circulating RNA Isolation Mini Kit
Targets
Circulating RNA, Exosomal RNA, miRNA
Circulating RNA, Exosomal RNA, miRNA
Sample Type
Plasma & serum
Plasma & serum
Time to Sample
35-40 min
35-40 min
Scientific Score
6.17
Brand
Norgen Biotek
Sizes
1 (50 Reactions)
Available From
1 Supplier
Price
$ 1,494.00
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Set-up of specialized techniques requires a certain input material or system; this is where sample preparation and gene editing are required, either to create a pure sample containing the target molecule or a genetic model with a particular behavior.

Preparation

Sample preparation is necessary for the majority of life science research experiments; enabling compatibility and accuracy while preventing interference from contaminants for successful analysis.

Initial steps include DNA or RNA Extraction (which can include the use of Columns & Beads), Protein Purification and Immunoprecipitation for isolation of the target molecule.

Sample cleanup and enrichment kits go one step further to increase concentration and remove any remaining impurities which may prevent proper analysis.

Sample preservation allows the prepared target molecule to be stabilized and stored until required; preventing degradation in the process.

Gene Editing

Genetic manipulation has recently been revolutionized by the discovery of CRISPR, allowing greater precision in gene editing than ever before.

Of course existing gene transfer techniques such as transfection, transformation, and transduction, are used to good effect. While Gene modification utilizes mutagenesis and bisulfite conversion to modify existing DNA, rather than introducing a new fragment. Gene silencing is slightly different in this respect, in that it negatively influences the expression of existing DNA via the use of short segments of DNA (shRNA, siRNA, and miRNA).

Gene editing controls offer a standardized model for comparison against the effects produced by the introduced genetic alteration. They, therefore, provide a test of reliability and accurate evaluation.

In vitro transcription produces a required RNA sample from existing DNA, useful for gene expression studies.